Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21130 and RR42_RS21135 are separated by 43 nucleotides RR42_RS21135 and RR42_RS21140 are separated by 307 nucleotides
RR42_RS21130: RR42_RS21130 - ABC transporter permease, at 65,756 to 66,892
_RS21130
RR42_RS21135: RR42_RS21135 - gluconate 5-dehydrogenase, at 66,936 to 67,703
_RS21135
RR42_RS21140: RR42_RS21140 - LysR family transcriptional regulator, at 68,011 to 68,904
_RS21140
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 65.962 kb on - strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 66.076 kb on - strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.199 kb on - strand, within RR42_RS21130 at 66.209 kb on + strand, within RR42_RS21130 at 66.279 kb on - strand, within RR42_RS21130 at 66.545 kb on - strand, within RR42_RS21130 at 66.635 kb on + strand, within RR42_RS21130 at 66.788 kb on + strand at 66.890 kb on + strand at 66.925 kb on - strand at 66.926 kb on + strand at 66.941 kb on + strand at 66.941 kb on + strand at 66.942 kb on - strand at 66.942 kb on - strand at 66.942 kb on - strand at 67.241 kb on + strand, within RR42_RS21135 at 67.242 kb on - strand, within RR42_RS21135 at 67.242 kb on - strand, within RR42_RS21135 at 67.411 kb on - strand, within RR42_RS21135 at 67.418 kb on + strand, within RR42_RS21135 at 67.506 kb on + strand, within RR42_RS21135 at 67.507 kb on - strand, within RR42_RS21135 at 67.650 kb on + strand at 67.650 kb on + strand at 67.655 kb on + strand at 67.656 kb on - strand at 67.656 kb on - strand at 67.748 kb on - strand at 67.819 kb on + strand at 67.868 kb on - strand at 67.878 kb on - strand at 68.325 kb on + strand, within RR42_RS21140 at 68.326 kb on - strand, within RR42_RS21140 at 68.417 kb on - strand, within RR42_RS21140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 65,962 - RR42_RS21130 0.18 +1.2 65,990 + RR42_RS21130 0.21 -1.8 65,990 + RR42_RS21130 0.21 +1.0 65,991 - RR42_RS21130 0.21 -0.4 65,991 - RR42_RS21130 0.21 +0.2 66,076 - RR42_RS21130 0.28 +0.8 66,198 + RR42_RS21130 0.39 -2.3 66,198 + RR42_RS21130 0.39 -0.3 66,198 + RR42_RS21130 0.39 -0.5 66,199 - RR42_RS21130 0.39 -0.1 66,209 + RR42_RS21130 0.40 -1.1 66,279 - RR42_RS21130 0.46 -0.8 66,545 - RR42_RS21130 0.69 +0.7 66,635 + RR42_RS21130 0.77 +0.1 66,788 + -0.3 66,890 + -0.5 66,925 - +0.1 66,926 + -0.7 66,941 + +0.9 66,941 + +2.3 66,942 - -0.5 66,942 - +0.3 66,942 - -0.5 67,241 + RR42_RS21135 0.40 +0.0 67,242 - RR42_RS21135 0.40 -0.3 67,242 - RR42_RS21135 0.40 -0.9 67,411 - RR42_RS21135 0.62 -0.4 67,418 + RR42_RS21135 0.63 +0.5 67,506 + RR42_RS21135 0.74 +0.4 67,507 - RR42_RS21135 0.74 -0.5 67,650 + -1.3 67,650 + +0.6 67,655 + +0.9 67,656 - -0.1 67,656 - +0.4 67,748 - +0.4 67,819 + +0.1 67,868 - -0.2 67,878 - -0.6 68,325 + RR42_RS21140 0.35 +1.2 68,326 - RR42_RS21140 0.35 -0.6 68,417 - RR42_RS21140 0.45 +0.3
Or see this region's nucleotide sequence