Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20810 and RR42_RS20815 are separated by 255 nucleotides RR42_RS20815 and RR42_RS20820 are separated by 65 nucleotides RR42_RS20820 and RR42_RS20825 are separated by 196 nucleotides RR42_RS20825 and RR42_RS20830 are separated by 217 nucleotides
RR42_RS20810: RR42_RS20810 - hypothetical protein, at 4,517,025 to 4,517,795
_RS20810
RR42_RS20815: RR42_RS20815 - phenylacetic acid degradation protein PaaI, at 4,518,051 to 4,518,488
_RS20815
RR42_RS20820: RR42_RS20820 - glyoxalase, at 4,518,554 to 4,518,940
_RS20820
RR42_RS20825: RR42_RS20825 - addiction module antitoxin, at 4,519,137 to 4,519,373
_RS20825
RR42_RS20830: RR42_RS20830 - insertase, at 4,519,591 to 4,521,261
_RS20830
Position (kb) 4518
4519 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4517.617 kb on - strand, within RR42_RS20810 at 4517.814 kb on + strand at 4517.814 kb on + strand at 4517.814 kb on + strand at 4517.815 kb on - strand at 4517.818 kb on + strand at 4517.819 kb on - strand at 4517.819 kb on - strand at 4517.829 kb on - strand at 4517.829 kb on - strand at 4517.899 kb on - strand at 4517.909 kb on + strand at 4517.918 kb on + strand at 4518.130 kb on + strand, within RR42_RS20815 at 4518.130 kb on + strand, within RR42_RS20815 at 4518.130 kb on + strand, within RR42_RS20815 at 4518.370 kb on + strand, within RR42_RS20815 at 4518.371 kb on - strand, within RR42_RS20815 at 4518.608 kb on + strand, within RR42_RS20820 at 4518.609 kb on - strand, within RR42_RS20820 at 4518.609 kb on - strand, within RR42_RS20820 at 4518.609 kb on - strand, within RR42_RS20820 at 4518.797 kb on + strand, within RR42_RS20820 at 4518.797 kb on + strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.798 kb on - strand, within RR42_RS20820 at 4518.800 kb on + strand, within RR42_RS20820 at 4518.800 kb on + strand, within RR42_RS20820 at 4518.800 kb on + strand, within RR42_RS20820 at 4518.800 kb on + strand, within RR42_RS20820 at 4518.801 kb on - strand, within RR42_RS20820 at 4518.801 kb on - strand, within RR42_RS20820 at 4518.801 kb on - strand, within RR42_RS20820 at 4518.860 kb on + strand, within RR42_RS20820 at 4518.860 kb on + strand, within RR42_RS20820 at 4518.861 kb on - strand, within RR42_RS20820 at 4518.861 kb on - strand, within RR42_RS20820 at 4518.910 kb on + strand at 4518.910 kb on + strand at 4518.941 kb on + strand at 4518.989 kb on + strand at 4519.061 kb on + strand at 4519.061 kb on + strand at 4519.062 kb on - strand at 4519.098 kb on - strand at 4519.098 kb on - strand at 4519.098 kb on - strand at 4519.282 kb on + strand, within RR42_RS20825 at 4519.282 kb on + strand, within RR42_RS20825 at 4519.283 kb on - strand, within RR42_RS20825 at 4519.358 kb on + strand at 4519.358 kb on + strand at 4519.358 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,517,617 - RR42_RS20810 0.77 -0.1 4,517,814 + -0.1 4,517,814 + -1.7 4,517,814 + -1.8 4,517,815 - +1.4 4,517,818 + +0.2 4,517,819 - -3.1 4,517,819 - -0.5 4,517,829 - -0.1 4,517,829 - +0.4 4,517,899 - +0.5 4,517,909 + -1.1 4,517,918 + -0.3 4,518,130 + RR42_RS20815 0.18 -0.7 4,518,130 + RR42_RS20815 0.18 -0.2 4,518,130 + RR42_RS20815 0.18 -1.7 4,518,370 + RR42_RS20815 0.73 +0.1 4,518,371 - RR42_RS20815 0.73 +0.4 4,518,608 + RR42_RS20820 0.14 +0.4 4,518,609 - RR42_RS20820 0.14 -0.5 4,518,609 - RR42_RS20820 0.14 +1.0 4,518,609 - RR42_RS20820 0.14 -0.1 4,518,797 + RR42_RS20820 0.63 -0.9 4,518,797 + RR42_RS20820 0.63 +1.4 4,518,798 - RR42_RS20820 0.63 +0.4 4,518,798 - RR42_RS20820 0.63 +0.4 4,518,798 - RR42_RS20820 0.63 -1.4 4,518,798 - RR42_RS20820 0.63 -1.4 4,518,798 - RR42_RS20820 0.63 +0.8 4,518,798 - RR42_RS20820 0.63 +0.4 4,518,800 + RR42_RS20820 0.64 -1.1 4,518,800 + RR42_RS20820 0.64 +0.3 4,518,800 + RR42_RS20820 0.64 -1.6 4,518,800 + RR42_RS20820 0.64 -0.1 4,518,801 - RR42_RS20820 0.64 +1.2 4,518,801 - RR42_RS20820 0.64 -0.4 4,518,801 - RR42_RS20820 0.64 +0.6 4,518,860 + RR42_RS20820 0.79 +0.9 4,518,860 + RR42_RS20820 0.79 -1.3 4,518,861 - RR42_RS20820 0.79 -0.9 4,518,861 - RR42_RS20820 0.79 -0.8 4,518,910 + +1.7 4,518,910 + +1.6 4,518,941 + +1.6 4,518,989 + +0.2 4,519,061 + -0.6 4,519,061 + -1.0 4,519,062 - +0.1 4,519,098 - -1.2 4,519,098 - +0.8 4,519,098 - +0.2 4,519,282 + RR42_RS20825 0.61 -1.2 4,519,282 + RR42_RS20825 0.61 -0.5 4,519,283 - RR42_RS20825 0.62 -0.6 4,519,358 + -1.4 4,519,358 + +1.0 4,519,358 + +0.8
Or see this region's nucleotide sequence