Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20450 and RR42_RS20455 are separated by 232 nucleotides RR42_RS20455 and RR42_RS20460 are separated by 185 nucleotides RR42_RS20460 and RR42_RS20465 are separated by 93 nucleotides
RR42_RS20450: RR42_RS20450 - 16S rRNA methyltransferase, at 4,430,174 to 4,431,526
_RS20450
RR42_RS20455: RR42_RS20455 - protease HtpX, at 4,431,759 to 4,432,619
_RS20455
RR42_RS20460: RR42_RS20460 - lysine transporter LysE, at 4,432,805 to 4,433,446
_RS20460
RR42_RS20465: RR42_RS20465 - methionyl-tRNA formyltransferase, at 4,433,540 to 4,434,553
_RS20465
Position (kb)
4431
4432
4433 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4430.926 kb on - strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.217 kb on - strand, within RR42_RS20450 at 4431.548 kb on + strand at 4431.548 kb on + strand at 4431.549 kb on - strand at 4431.658 kb on - strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.665 kb on - strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.770 kb on - strand at 4431.770 kb on - strand at 4431.935 kb on - strand, within RR42_RS20455 at 4432.354 kb on + strand, within RR42_RS20455 at 4432.408 kb on + strand, within RR42_RS20455 at 4432.450 kb on + strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.678 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.704 kb on + strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.705 kb on - strand at 4432.708 kb on + strand at 4432.708 kb on + strand at 4432.709 kb on - strand at 4432.711 kb on + strand at 4432.806 kb on + strand at 4432.807 kb on - strand at 4433.199 kb on + strand, within RR42_RS20460 at 4433.227 kb on + strand, within RR42_RS20460 at 4433.228 kb on - strand, within RR42_RS20460 at 4433.365 kb on + strand, within RR42_RS20460 at 4433.366 kb on - strand, within RR42_RS20460 at 4433.366 kb on - strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.367 kb on + strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.368 kb on - strand, within RR42_RS20460 at 4433.434 kb on + strand at 4433.434 kb on + strand at 4433.435 kb on - strand at 4433.435 kb on - strand at 4433.469 kb on + strand at 4433.470 kb on - strand at 4433.541 kb on + strand at 4433.541 kb on + strand at 4433.541 kb on + strand at 4433.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,430,926 - RR42_RS20450 0.56 -2.5 4,431,216 + RR42_RS20450 0.77 -3.5 4,431,216 + RR42_RS20450 0.77 +1.0 4,431,216 + RR42_RS20450 0.77 -2.5 4,431,217 - RR42_RS20450 0.77 -4.3 4,431,548 + -3.0 4,431,548 + -2.4 4,431,549 - -0.8 4,431,658 - -0.2 4,431,664 + -1.0 4,431,664 + +0.9 4,431,664 + -0.3 4,431,664 + -0.9 4,431,664 + +0.4 4,431,664 + +0.4 4,431,665 - -0.9 4,431,760 + -0.5 4,431,760 + -0.3 4,431,760 + -1.3 4,431,760 + -0.7 4,431,760 + +1.4 4,431,760 + -1.3 4,431,760 + -0.6 4,431,760 + +0.2 4,431,761 - -0.9 4,431,761 - +0.5 4,431,761 - -0.2 4,431,761 - -0.3 4,431,761 - -1.2 4,431,761 - -0.4 4,431,761 - -2.1 4,431,770 - -0.9 4,431,770 - -0.5 4,431,935 - RR42_RS20455 0.20 +0.8 4,432,354 + RR42_RS20455 0.69 -0.5 4,432,408 + RR42_RS20455 0.75 +0.4 4,432,450 + RR42_RS20455 0.80 +0.1 4,432,451 - RR42_RS20455 0.80 +0.4 4,432,451 - RR42_RS20455 0.80 +1.1 4,432,451 - RR42_RS20455 0.80 +0.3 4,432,451 - RR42_RS20455 0.80 +0.5 4,432,451 - RR42_RS20455 0.80 -0.1 4,432,483 + RR42_RS20455 0.84 -3.1 4,432,483 + RR42_RS20455 0.84 -0.7 4,432,483 + RR42_RS20455 0.84 +0.6 4,432,484 - RR42_RS20455 0.84 +0.5 4,432,484 - RR42_RS20455 0.84 -1.2 4,432,484 - RR42_RS20455 0.84 +1.8 4,432,678 + +0.2 4,432,704 + -0.5 4,432,704 + +0.8 4,432,704 + -0.2 4,432,704 + -1.7 4,432,705 - -1.1 4,432,705 - -0.6 4,432,705 - +0.9 4,432,705 - +0.5 4,432,708 + -0.6 4,432,708 + -0.0 4,432,709 - +0.2 4,432,711 + -0.4 4,432,806 + +0.1 4,432,807 - +0.3 4,433,199 + RR42_RS20460 0.61 +0.3 4,433,227 + RR42_RS20460 0.66 -0.4 4,433,228 - RR42_RS20460 0.66 +1.2 4,433,365 + RR42_RS20460 0.87 -0.6 4,433,366 - RR42_RS20460 0.87 -1.0 4,433,366 - RR42_RS20460 0.87 +0.6 4,433,367 + RR42_RS20460 0.88 +0.5 4,433,367 + RR42_RS20460 0.88 -0.0 4,433,367 + RR42_RS20460 0.88 -0.4 4,433,367 + RR42_RS20460 0.88 -0.9 4,433,368 - RR42_RS20460 0.88 -0.4 4,433,368 - RR42_RS20460 0.88 -0.6 4,433,368 - RR42_RS20460 0.88 +0.2 4,433,368 - RR42_RS20460 0.88 +0.4 4,433,368 - RR42_RS20460 0.88 -0.2 4,433,434 + -0.6 4,433,434 + -0.3 4,433,435 - +1.2 4,433,435 - +1.1 4,433,469 + -1.6 4,433,470 - -1.4 4,433,541 + -0.4 4,433,541 + -0.3 4,433,541 + -0.2 4,433,542 - -0.2
Or see this region's nucleotide sequence