Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20120 and RR42_RS20125 are separated by 145 nucleotides RR42_RS20125 and RR42_RS20130 are separated by 203 nucleotides
RR42_RS20120: RR42_RS20120 - amino acid ABC transporter substrate-binding protein, at 4,347,995 to 4,349,146
_RS20120
RR42_RS20125: RR42_RS20125 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 4,349,292 to 4,353,293
_RS20125
RR42_RS20130: RR42_RS20130 - primosome assembly protein PriA, at 4,353,497 to 4,355,800
_RS20130
Position (kb)
4349
4350
4351
4352
4353
4354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4348.606 kb on - strand, within RR42_RS20120 at 4348.737 kb on + strand, within RR42_RS20120 at 4348.737 kb on + strand, within RR42_RS20120 at 4348.738 kb on - strand, within RR42_RS20120 at 4348.738 kb on - strand, within RR42_RS20120 at 4348.785 kb on + strand, within RR42_RS20120 at 4348.785 kb on + strand, within RR42_RS20120 at 4348.786 kb on - strand, within RR42_RS20120 at 4348.917 kb on - strand, within RR42_RS20120 at 4348.917 kb on - strand, within RR42_RS20120 at 4349.008 kb on - strand, within RR42_RS20120 at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.161 kb on - strand at 4349.161 kb on - strand at 4349.161 kb on - strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.171 kb on + strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.173 kb on + strand at 4349.174 kb on - strand at 4349.236 kb on - strand at 4349.236 kb on - strand at 4349.382 kb on - strand at 4349.451 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.625 kb on + strand at 4349.701 kb on + strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.959 kb on + strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4350.041 kb on + strand, within RR42_RS20125 at 4350.042 kb on - strand, within RR42_RS20125 at 4350.042 kb on - strand, within RR42_RS20125 at 4350.056 kb on - strand, within RR42_RS20125 at 4350.356 kb on + strand, within RR42_RS20125 at 4351.012 kb on + strand, within RR42_RS20125 at 4351.015 kb on + strand, within RR42_RS20125 at 4351.015 kb on + strand, within RR42_RS20125 at 4351.447 kb on + strand, within RR42_RS20125 at 4351.448 kb on - strand, within RR42_RS20125 at 4351.448 kb on - strand, within RR42_RS20125 at 4351.628 kb on - strand, within RR42_RS20125 at 4351.663 kb on + strand, within RR42_RS20125 at 4351.759 kb on + strand, within RR42_RS20125 at 4351.760 kb on - strand, within RR42_RS20125 at 4351.771 kb on + strand, within RR42_RS20125 at 4351.771 kb on + strand, within RR42_RS20125 at 4351.772 kb on - strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.774 kb on + strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.775 kb on - strand, within RR42_RS20125 at 4351.795 kb on + strand, within RR42_RS20125 at 4351.795 kb on + strand, within RR42_RS20125 at 4351.795 kb on + strand, within RR42_RS20125 at 4351.795 kb on + strand, within RR42_RS20125 at 4351.796 kb on - strand, within RR42_RS20125 at 4351.796 kb on - strand, within RR42_RS20125 at 4351.796 kb on - strand, within RR42_RS20125 at 4351.796 kb on - strand, within RR42_RS20125 at 4351.840 kb on + strand, within RR42_RS20125 at 4351.840 kb on + strand, within RR42_RS20125 at 4351.881 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.885 kb on + strand, within RR42_RS20125 at 4351.886 kb on - strand, within RR42_RS20125 at 4351.898 kb on + strand, within RR42_RS20125 at 4351.899 kb on - strand, within RR42_RS20125 at 4351.900 kb on + strand, within RR42_RS20125 at 4351.900 kb on + strand, within RR42_RS20125 at 4351.900 kb on + strand, within RR42_RS20125 at 4351.901 kb on - strand, within RR42_RS20125 at 4351.901 kb on - strand, within RR42_RS20125 at 4351.901 kb on - strand, within RR42_RS20125 at 4351.916 kb on - strand, within RR42_RS20125 at 4351.924 kb on + strand, within RR42_RS20125 at 4352.036 kb on - strand, within RR42_RS20125 at 4352.036 kb on - strand, within RR42_RS20125 at 4352.177 kb on + strand, within RR42_RS20125 at 4352.178 kb on - strand, within RR42_RS20125 at 4352.194 kb on + strand, within RR42_RS20125 at 4352.194 kb on + strand, within RR42_RS20125 at 4352.195 kb on - strand, within RR42_RS20125 at 4352.233 kb on + strand, within RR42_RS20125 at 4352.234 kb on - strand, within RR42_RS20125 at 4352.234 kb on - strand, within RR42_RS20125 at 4352.234 kb on - strand, within RR42_RS20125 at 4352.234 kb on - strand, within RR42_RS20125 at 4352.270 kb on + strand, within RR42_RS20125 at 4352.270 kb on + strand, within RR42_RS20125 at 4352.271 kb on - strand, within RR42_RS20125 at 4352.271 kb on - strand, within RR42_RS20125 at 4352.273 kb on - strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.320 kb on + strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.321 kb on - strand, within RR42_RS20125 at 4352.371 kb on + strand, within RR42_RS20125 at 4352.371 kb on + strand, within RR42_RS20125 at 4352.372 kb on - strand, within RR42_RS20125 at 4352.383 kb on + strand, within RR42_RS20125 at 4352.383 kb on + strand, within RR42_RS20125 at 4352.383 kb on + strand, within RR42_RS20125 at 4352.383 kb on + strand, within RR42_RS20125 at 4352.383 kb on + strand, within RR42_RS20125 at 4352.431 kb on + strand, within RR42_RS20125 at 4352.431 kb on + strand, within RR42_RS20125 at 4352.431 kb on + strand, within RR42_RS20125 at 4352.431 kb on + strand, within RR42_RS20125 at 4352.458 kb on + strand, within RR42_RS20125 at 4352.458 kb on + strand, within RR42_RS20125 at 4352.458 kb on + strand, within RR42_RS20125 at 4352.459 kb on - strand, within RR42_RS20125 at 4352.618 kb on + strand, within RR42_RS20125 at 4352.618 kb on + strand, within RR42_RS20125 at 4352.619 kb on - strand, within RR42_RS20125 at 4352.619 kb on - strand, within RR42_RS20125 at 4353.057 kb on + strand at 4353.175 kb on + strand at 4353.175 kb on + strand at 4353.176 kb on - strand at 4353.310 kb on - strand at 4353.310 kb on - strand at 4353.310 kb on - strand at 4353.365 kb on + strand at 4353.365 kb on + strand at 4353.366 kb on - strand at 4353.392 kb on + strand at 4353.396 kb on + strand at 4353.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,348,606 - RR42_RS20120 0.53 +0.2 4,348,737 + RR42_RS20120 0.64 +1.0 4,348,737 + RR42_RS20120 0.64 -1.2 4,348,738 - RR42_RS20120 0.64 -0.1 4,348,738 - RR42_RS20120 0.64 +0.4 4,348,785 + RR42_RS20120 0.69 -0.1 4,348,785 + RR42_RS20120 0.69 -0.2 4,348,786 - RR42_RS20120 0.69 -1.1 4,348,917 - RR42_RS20120 0.80 +0.4 4,348,917 - RR42_RS20120 0.80 +0.6 4,349,008 - RR42_RS20120 0.88 +1.4 4,349,160 + +0.2 4,349,160 + +0.7 4,349,160 + +1.1 4,349,160 + +1.3 4,349,161 - +0.1 4,349,161 - +0.4 4,349,161 - -1.0 4,349,164 + +0.5 4,349,164 + +0.3 4,349,164 + +0.3 4,349,164 + +0.7 4,349,165 - +0.8 4,349,165 - +1.4 4,349,165 - -0.7 4,349,165 - +0.1 4,349,165 - -0.2 4,349,165 - +0.9 4,349,165 - -0.6 4,349,165 - -0.2 4,349,171 + +0.9 4,349,172 - +1.4 4,349,172 - +0.6 4,349,172 - -0.5 4,349,172 - +0.2 4,349,172 - -2.8 4,349,172 - +2.2 4,349,173 + -0.5 4,349,174 - +0.6 4,349,236 - -0.2 4,349,236 - +0.8 4,349,382 - -1.9 4,349,451 + +0.9 4,349,547 + -0.3 4,349,547 + +0.3 4,349,547 + +1.4 4,349,547 + +1.1 4,349,548 - +0.6 4,349,548 - -0.8 4,349,548 - -1.0 4,349,548 - +0.2 4,349,625 + +0.7 4,349,701 + RR42_RS20125 0.10 -1.3 4,349,702 - RR42_RS20125 0.10 -0.2 4,349,702 - RR42_RS20125 0.10 +0.4 4,349,702 - RR42_RS20125 0.10 +0.2 4,349,702 - RR42_RS20125 0.10 -0.8 4,349,702 - RR42_RS20125 0.10 +1.8 4,349,959 + RR42_RS20125 0.17 +0.7 4,349,960 - RR42_RS20125 0.17 +0.8 4,349,960 - RR42_RS20125 0.17 -0.4 4,349,960 - RR42_RS20125 0.17 -0.2 4,349,960 - RR42_RS20125 0.17 +0.9 4,350,041 + RR42_RS20125 0.19 -0.1 4,350,042 - RR42_RS20125 0.19 -0.4 4,350,042 - RR42_RS20125 0.19 +0.5 4,350,056 - RR42_RS20125 0.19 +0.1 4,350,356 + RR42_RS20125 0.27 +0.8 4,351,012 + RR42_RS20125 0.43 +0.5 4,351,015 + RR42_RS20125 0.43 +0.2 4,351,015 + RR42_RS20125 0.43 +0.3 4,351,447 + RR42_RS20125 0.54 -0.7 4,351,448 - RR42_RS20125 0.54 -0.8 4,351,448 - RR42_RS20125 0.54 +0.6 4,351,628 - RR42_RS20125 0.58 +0.4 4,351,663 + RR42_RS20125 0.59 +2.6 4,351,759 + RR42_RS20125 0.62 -0.6 4,351,760 - RR42_RS20125 0.62 -1.4 4,351,771 + RR42_RS20125 0.62 +0.7 4,351,771 + RR42_RS20125 0.62 +0.0 4,351,772 - RR42_RS20125 0.62 +0.7 4,351,774 + RR42_RS20125 0.62 -1.1 4,351,774 + RR42_RS20125 0.62 +0.6 4,351,774 + RR42_RS20125 0.62 +0.5 4,351,774 + RR42_RS20125 0.62 +1.1 4,351,774 + RR42_RS20125 0.62 -1.2 4,351,774 + RR42_RS20125 0.62 -0.7 4,351,774 + RR42_RS20125 0.62 -0.2 4,351,774 + RR42_RS20125 0.62 +1.6 4,351,774 + RR42_RS20125 0.62 +1.4 4,351,774 + RR42_RS20125 0.62 +0.2 4,351,774 + RR42_RS20125 0.62 +0.8 4,351,774 + RR42_RS20125 0.62 -0.4 4,351,774 + RR42_RS20125 0.62 +0.4 4,351,774 + RR42_RS20125 0.62 +1.2 4,351,774 + RR42_RS20125 0.62 +1.7 4,351,775 - RR42_RS20125 0.62 +1.0 4,351,775 - RR42_RS20125 0.62 -0.2 4,351,775 - RR42_RS20125 0.62 -0.5 4,351,775 - RR42_RS20125 0.62 +0.1 4,351,775 - RR42_RS20125 0.62 +1.6 4,351,775 - RR42_RS20125 0.62 +0.6 4,351,775 - RR42_RS20125 0.62 +1.1 4,351,795 + RR42_RS20125 0.63 +1.3 4,351,795 + RR42_RS20125 0.63 -0.4 4,351,795 + RR42_RS20125 0.63 +0.6 4,351,795 + RR42_RS20125 0.63 +0.0 4,351,796 - RR42_RS20125 0.63 +0.0 4,351,796 - RR42_RS20125 0.63 +0.4 4,351,796 - RR42_RS20125 0.63 +0.8 4,351,796 - RR42_RS20125 0.63 +0.4 4,351,840 + RR42_RS20125 0.64 +0.1 4,351,840 + RR42_RS20125 0.64 -0.1 4,351,881 + RR42_RS20125 0.65 +2.0 4,351,885 + RR42_RS20125 0.65 +1.2 4,351,885 + RR42_RS20125 0.65 +3.3 4,351,885 + RR42_RS20125 0.65 +1.0 4,351,885 + RR42_RS20125 0.65 +1.7 4,351,885 + RR42_RS20125 0.65 +0.7 4,351,885 + RR42_RS20125 0.65 -1.8 4,351,885 + RR42_RS20125 0.65 +1.1 4,351,886 - RR42_RS20125 0.65 -0.0 4,351,898 + RR42_RS20125 0.65 +1.5 4,351,899 - RR42_RS20125 0.65 +1.0 4,351,900 + RR42_RS20125 0.65 +1.8 4,351,900 + RR42_RS20125 0.65 +0.8 4,351,900 + RR42_RS20125 0.65 +0.8 4,351,901 - RR42_RS20125 0.65 -0.6 4,351,901 - RR42_RS20125 0.65 -1.8 4,351,901 - RR42_RS20125 0.65 +0.4 4,351,916 - RR42_RS20125 0.66 +0.7 4,351,924 + RR42_RS20125 0.66 -0.5 4,352,036 - RR42_RS20125 0.69 +0.6 4,352,036 - RR42_RS20125 0.69 -0.2 4,352,177 + RR42_RS20125 0.72 +1.6 4,352,178 - RR42_RS20125 0.72 +2.4 4,352,194 + RR42_RS20125 0.73 +0.1 4,352,194 + RR42_RS20125 0.73 +1.2 4,352,195 - RR42_RS20125 0.73 -1.8 4,352,233 + RR42_RS20125 0.73 -2.4 4,352,234 - RR42_RS20125 0.74 -1.2 4,352,234 - RR42_RS20125 0.74 +0.3 4,352,234 - RR42_RS20125 0.74 +0.4 4,352,234 - RR42_RS20125 0.74 -3.3 4,352,270 + RR42_RS20125 0.74 +0.3 4,352,270 + RR42_RS20125 0.74 +1.5 4,352,271 - RR42_RS20125 0.74 -0.4 4,352,271 - RR42_RS20125 0.74 -0.4 4,352,273 - RR42_RS20125 0.74 -0.5 4,352,320 + RR42_RS20125 0.76 -0.6 4,352,320 + RR42_RS20125 0.76 +1.2 4,352,320 + RR42_RS20125 0.76 +0.1 4,352,320 + RR42_RS20125 0.76 +1.0 4,352,320 + RR42_RS20125 0.76 +0.4 4,352,320 + RR42_RS20125 0.76 +0.1 4,352,320 + RR42_RS20125 0.76 +0.1 4,352,320 + RR42_RS20125 0.76 -0.7 4,352,320 + RR42_RS20125 0.76 +1.0 4,352,320 + RR42_RS20125 0.76 +0.5 4,352,321 - RR42_RS20125 0.76 +0.6 4,352,321 - RR42_RS20125 0.76 -3.1 4,352,321 - RR42_RS20125 0.76 -0.5 4,352,321 - RR42_RS20125 0.76 +0.7 4,352,321 - RR42_RS20125 0.76 -0.2 4,352,321 - RR42_RS20125 0.76 -1.4 4,352,321 - RR42_RS20125 0.76 +0.2 4,352,321 - RR42_RS20125 0.76 -0.8 4,352,371 + RR42_RS20125 0.77 -0.7 4,352,371 + RR42_RS20125 0.77 +2.4 4,352,372 - RR42_RS20125 0.77 +0.7 4,352,383 + RR42_RS20125 0.77 +0.2 4,352,383 + RR42_RS20125 0.77 -0.0 4,352,383 + RR42_RS20125 0.77 -0.4 4,352,383 + RR42_RS20125 0.77 +0.5 4,352,383 + RR42_RS20125 0.77 +1.1 4,352,431 + RR42_RS20125 0.78 -0.1 4,352,431 + RR42_RS20125 0.78 -0.7 4,352,431 + RR42_RS20125 0.78 +0.4 4,352,431 + RR42_RS20125 0.78 -0.7 4,352,458 + RR42_RS20125 0.79 +1.4 4,352,458 + RR42_RS20125 0.79 -0.8 4,352,458 + RR42_RS20125 0.79 +1.4 4,352,459 - RR42_RS20125 0.79 -0.5 4,352,618 + RR42_RS20125 0.83 +0.9 4,352,618 + RR42_RS20125 0.83 -1.6 4,352,619 - RR42_RS20125 0.83 +0.6 4,352,619 - RR42_RS20125 0.83 +0.6 4,353,057 + +0.1 4,353,175 + +0.0 4,353,175 + -1.1 4,353,176 - +0.6 4,353,310 - -1.9 4,353,310 - -0.9 4,353,310 - +1.1 4,353,365 + -0.4 4,353,365 + +0.2 4,353,366 - -3.5 4,353,392 + +0.2 4,353,396 + -1.6 4,353,397 - -0.2
Or see this region's nucleotide sequence