Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19005 and RR42_RS19010 overlap by 14 nucleotides RR42_RS19010 and RR42_RS19015 are separated by 246 nucleotides
RR42_RS19005: RR42_RS19005 - 3-dehydroquinate synthase, at 4,116,964 to 4,118,070
_RS19005
RR42_RS19010: RR42_RS19010 - shikimate kinase, at 4,118,057 to 4,118,671
_RS19010
RR42_RS19015: RR42_RS19015 - secretin, at 4,118,918 to 4,121,092
_RS19015
Position (kb)
4118
4119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4117.341 kb on + strand, within RR42_RS19005 at 4117.341 kb on + strand, within RR42_RS19005 at 4117.342 kb on - strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.719 kb on - strand, within RR42_RS19005 at 4117.926 kb on - strand, within RR42_RS19005 at 4118.033 kb on + strand at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.180 kb on - strand, within RR42_RS19010 at 4118.182 kb on + strand, within RR42_RS19010 at 4118.182 kb on + strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.263 kb on - strand, within RR42_RS19010 at 4118.263 kb on - strand, within RR42_RS19010 at 4118.365 kb on - strand, within RR42_RS19010 at 4118.531 kb on - strand, within RR42_RS19010 at 4118.580 kb on + strand, within RR42_RS19010 at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.857 kb on + strand at 4118.857 kb on + strand at 4118.894 kb on + strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.920 kb on - strand at 4119.076 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.078 kb on + strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.079 kb on - strand at 4119.306 kb on + strand, within RR42_RS19015 at 4119.307 kb on - strand, within RR42_RS19015 at 4119.343 kb on + strand, within RR42_RS19015 at 4119.343 kb on + strand, within RR42_RS19015 at 4119.343 kb on + strand, within RR42_RS19015 at 4119.343 kb on + strand, within RR42_RS19015 at 4119.344 kb on - strand, within RR42_RS19015 at 4119.344 kb on - strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.564 kb on + strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.565 kb on - strand, within RR42_RS19015 at 4119.637 kb on + strand, within RR42_RS19015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,117,341 + RR42_RS19005 0.34 +0.2 4,117,341 + RR42_RS19005 0.34 +0.3 4,117,342 - RR42_RS19005 0.34 -1.0 4,117,718 + RR42_RS19005 0.68 -0.7 4,117,718 + RR42_RS19005 0.68 +1.3 4,117,718 + RR42_RS19005 0.68 -1.0 4,117,719 - RR42_RS19005 0.68 +0.6 4,117,926 - RR42_RS19005 0.87 -0.1 4,118,033 + +1.3 4,118,179 + RR42_RS19010 0.20 -1.3 4,118,179 + RR42_RS19010 0.20 +0.3 4,118,179 + RR42_RS19010 0.20 -3.6 4,118,179 + RR42_RS19010 0.20 -2.9 4,118,179 + RR42_RS19010 0.20 -0.4 4,118,180 - RR42_RS19010 0.20 -1.3 4,118,182 + RR42_RS19010 0.20 -2.1 4,118,182 + RR42_RS19010 0.20 -0.8 4,118,183 - RR42_RS19010 0.20 +0.0 4,118,183 - RR42_RS19010 0.20 -0.8 4,118,183 - RR42_RS19010 0.20 -0.5 4,118,262 + RR42_RS19010 0.33 -2.9 4,118,262 + RR42_RS19010 0.33 +0.2 4,118,262 + RR42_RS19010 0.33 -1.4 4,118,263 - RR42_RS19010 0.33 -1.7 4,118,263 - RR42_RS19010 0.33 -1.0 4,118,365 - RR42_RS19010 0.50 +0.1 4,118,531 - RR42_RS19010 0.77 -1.6 4,118,580 + RR42_RS19010 0.85 -1.3 4,118,736 + -0.7 4,118,736 + -1.0 4,118,736 + -1.7 4,118,736 + -0.8 4,118,736 + -2.0 4,118,736 + -1.4 4,118,736 + -1.6 4,118,736 + -1.2 4,118,736 + -1.3 4,118,737 - +1.3 4,118,737 - +2.2 4,118,737 - -0.4 4,118,737 - +0.3 4,118,737 - +0.1 4,118,737 - -0.4 4,118,737 - -0.6 4,118,737 - +0.8 4,118,737 - +0.6 4,118,737 - +0.7 4,118,857 + +0.5 4,118,857 + +0.4 4,118,894 + +0.6 4,118,895 - -2.1 4,118,895 - -0.5 4,118,895 - +0.1 4,118,895 - -0.1 4,118,920 - -0.6 4,119,076 + +1.5 4,119,078 + +0.8 4,119,078 + +0.3 4,119,078 + +0.3 4,119,078 + -2.7 4,119,078 + +2.2 4,119,078 + +0.0 4,119,078 + -0.9 4,119,079 - +0.4 4,119,079 - +1.3 4,119,079 - -0.8 4,119,079 - +1.8 4,119,079 - +0.4 4,119,079 - -0.4 4,119,079 - +0.0 4,119,306 + RR42_RS19015 0.18 +0.2 4,119,307 - RR42_RS19015 0.18 -1.0 4,119,343 + RR42_RS19015 0.20 -0.5 4,119,343 + RR42_RS19015 0.20 +0.5 4,119,343 + RR42_RS19015 0.20 -1.0 4,119,343 + RR42_RS19015 0.20 +0.3 4,119,344 - RR42_RS19015 0.20 +1.5 4,119,344 - RR42_RS19015 0.20 +0.6 4,119,564 + RR42_RS19015 0.30 +2.6 4,119,564 + RR42_RS19015 0.30 -0.5 4,119,564 + RR42_RS19015 0.30 -0.1 4,119,564 + RR42_RS19015 0.30 +0.5 4,119,564 + RR42_RS19015 0.30 -2.4 4,119,564 + RR42_RS19015 0.30 -0.3 4,119,564 + RR42_RS19015 0.30 -1.3 4,119,565 - RR42_RS19015 0.30 -0.8 4,119,565 - RR42_RS19015 0.30 +1.0 4,119,565 - RR42_RS19015 0.30 +0.6 4,119,565 - RR42_RS19015 0.30 +1.0 4,119,565 - RR42_RS19015 0.30 -0.1 4,119,565 - RR42_RS19015 0.30 +0.1 4,119,637 + RR42_RS19015 0.33 -2.0
Or see this region's nucleotide sequence