Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18975 and RR42_RS18980 are separated by 228 nucleotides RR42_RS18980 and RR42_RS18985 are separated by 93 nucleotides RR42_RS18985 and RR42_RS18990 are separated by 133 nucleotides
RR42_RS18975: RR42_RS18975 - ABC transporter substrate-binding protein, at 4,106,189 to 4,107,292
_RS18975
RR42_RS18980: RR42_RS18980 - GCN5 family acetyltransferase, at 4,107,521 to 4,108,057
_RS18980
RR42_RS18985: RR42_RS18985 - glutamate synthase, at 4,108,151 to 4,109,614
_RS18985
RR42_RS18990: RR42_RS18990 - glutamate synthase, at 4,109,748 to 4,114,529
_RS18990
Position (kb)
4108
4109
4110 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4107.253 kb on + strand at 4107.254 kb on - strand at 4107.254 kb on - strand at 4107.254 kb on - strand at 4107.254 kb on - strand at 4107.254 kb on - strand at 4107.348 kb on + strand at 4107.348 kb on + strand at 4107.377 kb on + strand at 4107.377 kb on + strand at 4107.377 kb on + strand at 4107.377 kb on + strand at 4107.377 kb on + strand at 4107.394 kb on + strand at 4107.395 kb on - strand at 4107.395 kb on - strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.450 kb on + strand at 4107.451 kb on - strand at 4107.451 kb on - strand at 4107.451 kb on - strand at 4107.507 kb on + strand at 4107.507 kb on + strand at 4107.507 kb on + strand at 4107.507 kb on + strand at 4107.507 kb on + strand at 4107.508 kb on - strand at 4107.508 kb on - strand at 4107.508 kb on - strand at 4107.509 kb on + strand at 4107.530 kb on + strand at 4107.530 kb on + strand at 4107.530 kb on + strand at 4107.530 kb on + strand at 4107.531 kb on - strand at 4107.531 kb on - strand at 4107.531 kb on - strand at 4107.531 kb on - strand at 4107.531 kb on - strand at 4107.698 kb on + strand, within RR42_RS18980 at 4107.698 kb on + strand, within RR42_RS18980 at 4107.698 kb on + strand, within RR42_RS18980 at 4107.699 kb on - strand, within RR42_RS18980 at 4107.699 kb on - strand, within RR42_RS18980 at 4107.699 kb on - strand, within RR42_RS18980 at 4107.699 kb on - strand, within RR42_RS18980 at 4107.814 kb on + strand, within RR42_RS18980 at 4107.959 kb on + strand, within RR42_RS18980 at 4108.011 kb on - strand at 4108.011 kb on - strand at 4108.011 kb on - strand at 4108.011 kb on - strand at 4108.039 kb on + strand at 4108.068 kb on - strand at 4108.069 kb on + strand at 4108.293 kb on + strand at 4108.294 kb on - strand at 4108.294 kb on - strand at 4108.594 kb on - strand, within RR42_RS18985 at 4108.684 kb on + strand, within RR42_RS18985 at 4108.685 kb on - strand, within RR42_RS18985 at 4108.688 kb on + strand, within RR42_RS18985 at 4108.688 kb on + strand, within RR42_RS18985 at 4108.688 kb on + strand, within RR42_RS18985 at 4108.688 kb on + strand, within RR42_RS18985 at 4108.689 kb on - strand, within RR42_RS18985 at 4108.689 kb on - strand, within RR42_RS18985 at 4108.932 kb on + strand, within RR42_RS18985 at 4108.932 kb on + strand, within RR42_RS18985 at 4108.932 kb on + strand, within RR42_RS18985 at 4108.932 kb on + strand, within RR42_RS18985 at 4108.933 kb on - strand, within RR42_RS18985 at 4108.933 kb on - strand, within RR42_RS18985 at 4108.933 kb on - strand, within RR42_RS18985 at 4109.064 kb on + strand, within RR42_RS18985 at 4109.065 kb on - strand, within RR42_RS18985 at 4109.400 kb on + strand, within RR42_RS18985 at 4109.401 kb on - strand, within RR42_RS18985 at 4109.401 kb on - strand, within RR42_RS18985 at 4109.536 kb on - strand at 4109.536 kb on - strand at 4109.536 kb on - strand at 4109.566 kb on - strand at 4109.566 kb on - strand at 4109.676 kb on + strand at 4109.676 kb on + strand at 4109.800 kb on + strand at 4109.800 kb on + strand at 4109.800 kb on + strand at 4109.800 kb on + strand at 4109.801 kb on - strand at 4109.801 kb on - strand at 4109.801 kb on - strand at 4109.801 kb on - strand at 4109.825 kb on - strand at 4109.825 kb on - strand at 4110.086 kb on - strand at 4110.086 kb on - strand at 4110.086 kb on - strand at 4110.086 kb on - strand at 4110.086 kb on - strand at 4110.091 kb on + strand at 4110.091 kb on + strand at 4110.091 kb on + strand at 4110.091 kb on + strand at 4110.092 kb on - strand at 4110.092 kb on - strand at 4110.092 kb on - strand at 4110.092 kb on - strand at 4110.092 kb on - strand at 4110.167 kb on + strand at 4110.167 kb on + strand at 4110.167 kb on + strand at 4110.169 kb on + strand at 4110.169 kb on + strand at 4110.169 kb on + strand at 4110.170 kb on - strand at 4110.170 kb on - strand at 4110.284 kb on - strand, within RR42_RS18990 at 4110.385 kb on + strand, within RR42_RS18990 at 4110.386 kb on - strand, within RR42_RS18990 at 4110.386 kb on - strand, within RR42_RS18990 at 4110.386 kb on - strand, within RR42_RS18990 at 4110.402 kb on + strand, within RR42_RS18990 at 4110.403 kb on - strand, within RR42_RS18990 at 4110.403 kb on - strand, within RR42_RS18990 at 4110.508 kb on + strand, within RR42_RS18990 at 4110.564 kb on - strand, within RR42_RS18990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,107,253 + -1.7 4,107,254 - +0.1 4,107,254 - -0.8 4,107,254 - -0.7 4,107,254 - -1.8 4,107,254 - +0.3 4,107,348 + -0.1 4,107,348 + +0.8 4,107,377 + +0.1 4,107,377 + +0.2 4,107,377 + +1.3 4,107,377 + +0.2 4,107,377 + -1.3 4,107,394 + +1.1 4,107,395 - +0.4 4,107,395 - +1.3 4,107,450 + +0.6 4,107,450 + -0.3 4,107,450 + -0.5 4,107,450 + +0.0 4,107,450 + +1.0 4,107,450 + +2.1 4,107,450 + +1.8 4,107,450 + +0.1 4,107,451 - -0.8 4,107,451 - +2.9 4,107,451 - +0.8 4,107,507 + +0.4 4,107,507 + +0.7 4,107,507 + -0.0 4,107,507 + -0.3 4,107,507 + -1.6 4,107,508 - +0.5 4,107,508 - -0.2 4,107,508 - +0.7 4,107,509 + -1.0 4,107,530 + +1.3 4,107,530 + +0.2 4,107,530 + +0.0 4,107,530 + -0.4 4,107,531 - +0.6 4,107,531 - +0.2 4,107,531 - -0.6 4,107,531 - +1.0 4,107,531 - +0.1 4,107,698 + RR42_RS18980 0.33 +1.4 4,107,698 + RR42_RS18980 0.33 -0.4 4,107,698 + RR42_RS18980 0.33 -0.5 4,107,699 - RR42_RS18980 0.33 -0.7 4,107,699 - RR42_RS18980 0.33 -0.1 4,107,699 - RR42_RS18980 0.33 +0.5 4,107,699 - RR42_RS18980 0.33 +0.3 4,107,814 + RR42_RS18980 0.55 -1.0 4,107,959 + RR42_RS18980 0.82 -0.3 4,108,011 - -0.6 4,108,011 - -1.5 4,108,011 - -0.1 4,108,011 - +0.1 4,108,039 + +1.1 4,108,068 - +0.0 4,108,069 + +0.0 4,108,293 + +0.9 4,108,294 - -2.8 4,108,294 - -2.9 4,108,594 - RR42_RS18985 0.30 -1.3 4,108,684 + RR42_RS18985 0.36 -2.4 4,108,685 - RR42_RS18985 0.36 -1.1 4,108,688 + RR42_RS18985 0.37 -0.6 4,108,688 + RR42_RS18985 0.37 -1.4 4,108,688 + RR42_RS18985 0.37 -2.8 4,108,688 + RR42_RS18985 0.37 -3.2 4,108,689 - RR42_RS18985 0.37 -0.5 4,108,689 - RR42_RS18985 0.37 -0.5 4,108,932 + RR42_RS18985 0.53 -1.0 4,108,932 + RR42_RS18985 0.53 +1.3 4,108,932 + RR42_RS18985 0.53 -1.4 4,108,932 + RR42_RS18985 0.53 -4.2 4,108,933 - RR42_RS18985 0.53 -2.8 4,108,933 - RR42_RS18985 0.53 -0.5 4,108,933 - RR42_RS18985 0.53 -3.0 4,109,064 + RR42_RS18985 0.62 -1.7 4,109,065 - RR42_RS18985 0.62 -0.9 4,109,400 + RR42_RS18985 0.85 -0.7 4,109,401 - RR42_RS18985 0.85 -1.6 4,109,401 - RR42_RS18985 0.85 -2.5 4,109,536 - -2.0 4,109,536 - -3.1 4,109,536 - -2.3 4,109,566 - -2.7 4,109,566 - -1.3 4,109,676 + -1.1 4,109,676 + -1.4 4,109,800 + -4.5 4,109,800 + -1.6 4,109,800 + -3.0 4,109,800 + -2.6 4,109,801 - +0.5 4,109,801 - +1.2 4,109,801 - +0.6 4,109,801 - -0.7 4,109,825 - -1.0 4,109,825 - +0.7 4,110,086 - +0.8 4,110,086 - -1.0 4,110,086 - -1.0 4,110,086 - -0.8 4,110,086 - -0.9 4,110,091 + -0.7 4,110,091 + -0.3 4,110,091 + -0.1 4,110,091 + -0.2 4,110,092 - -0.9 4,110,092 - -1.1 4,110,092 - +3.6 4,110,092 - -0.8 4,110,092 - -1.1 4,110,167 + -0.8 4,110,167 + -1.8 4,110,167 + +0.7 4,110,169 + -1.4 4,110,169 + -0.9 4,110,169 + -2.0 4,110,170 - -0.4 4,110,170 - -1.3 4,110,284 - RR42_RS18990 0.11 +1.3 4,110,385 + RR42_RS18990 0.13 -1.2 4,110,386 - RR42_RS18990 0.13 +0.7 4,110,386 - RR42_RS18990 0.13 -1.8 4,110,386 - RR42_RS18990 0.13 -0.6 4,110,402 + RR42_RS18990 0.14 -1.1 4,110,403 - RR42_RS18990 0.14 -1.5 4,110,403 - RR42_RS18990 0.14 +1.3 4,110,508 + RR42_RS18990 0.16 -0.3 4,110,564 - RR42_RS18990 0.17 +2.1
Or see this region's nucleotide sequence