Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18830 and RR42_RS18835 are separated by 60 nucleotides RR42_RS18835 and RR42_RS18840 are separated by 19 nucleotides RR42_RS18840 and RR42_RS18845 are separated by 492 nucleotides
RR42_RS18830: RR42_RS18830 - large-conductance mechanosensitive channel, at 4,083,080 to 4,083,511
_RS18830
RR42_RS18835: RR42_RS18835 - Nif3-like dinuclear metal center hexameric protein, at 4,083,572 to 4,084,318
_RS18835
RR42_RS18840: RR42_RS18840 - 2-alkenal reductase, at 4,084,338 to 4,085,531
_RS18840
RR42_RS18845: RR42_RS18845 - membrane protein, at 4,086,024 to 4,087,124
_RS18845
Position (kb)
4084
4085
4086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.349 kb on + strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.350 kb on - strand, within RR42_RS18830 at 4083.590 kb on - strand at 4083.602 kb on - strand at 4083.602 kb on - strand at 4083.747 kb on + strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.748 kb on - strand, within RR42_RS18835 at 4083.783 kb on + strand, within RR42_RS18835 at 4084.032 kb on + strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.033 kb on - strand, within RR42_RS18835 at 4084.214 kb on + strand, within RR42_RS18835 at 4084.215 kb on - strand, within RR42_RS18835 at 4084.215 kb on - strand, within RR42_RS18835 at 4084.254 kb on + strand at 4084.254 kb on + strand at 4084.254 kb on + strand at 4084.255 kb on - strand at 4084.255 kb on - strand at 4084.263 kb on + strand at 4084.264 kb on - strand at 4084.294 kb on - strand at 4084.294 kb on - strand at 4084.294 kb on - strand at 4084.319 kb on - strand at 4084.319 kb on - strand at 4084.337 kb on + strand at 4084.337 kb on + strand at 4084.524 kb on + strand, within RR42_RS18840 at 4084.713 kb on + strand, within RR42_RS18840 at 4084.720 kb on + strand, within RR42_RS18840 at 4084.877 kb on + strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840 at 4085.101 kb on - strand, within RR42_RS18840 at 4085.370 kb on - strand, within RR42_RS18840 at 4085.465 kb on - strand at 4085.465 kb on - strand at 4085.465 kb on - strand at 4085.465 kb on - strand at 4085.465 kb on - strand at 4085.562 kb on + strand at 4085.563 kb on - strand at 4085.563 kb on - strand at 4085.570 kb on + strand at 4085.570 kb on + strand at 4085.570 kb on + strand at 4085.570 kb on + strand at 4085.571 kb on - strand at 4085.571 kb on - strand at 4085.571 kb on - strand at 4085.571 kb on - strand at 4085.634 kb on - strand at 4085.686 kb on + strand at 4085.754 kb on + strand at 4085.755 kb on - strand at 4085.853 kb on + strand at 4085.853 kb on + strand at 4085.853 kb on + strand at 4085.853 kb on + strand at 4085.853 kb on + strand at 4085.854 kb on - strand at 4085.854 kb on - strand at 4085.854 kb on - strand at 4085.883 kb on - strand at 4085.932 kb on + strand at 4085.998 kb on + strand at 4085.998 kb on + strand at 4085.998 kb on + strand at 4086.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,083,349 + RR42_RS18830 0.62 -0.7 4,083,349 + RR42_RS18830 0.62 +1.2 4,083,349 + RR42_RS18830 0.62 -0.6 4,083,350 - RR42_RS18830 0.62 +1.2 4,083,350 - RR42_RS18830 0.62 +0.6 4,083,350 - RR42_RS18830 0.62 +1.0 4,083,590 - +0.5 4,083,602 - +1.5 4,083,602 - -3.1 4,083,747 + RR42_RS18835 0.23 +0.0 4,083,748 - RR42_RS18835 0.24 -1.0 4,083,748 - RR42_RS18835 0.24 +0.3 4,083,748 - RR42_RS18835 0.24 -0.1 4,083,748 - RR42_RS18835 0.24 -0.1 4,083,748 - RR42_RS18835 0.24 +1.1 4,083,783 + RR42_RS18835 0.28 +0.0 4,084,032 + RR42_RS18835 0.62 +0.9 4,084,033 - RR42_RS18835 0.62 -0.0 4,084,033 - RR42_RS18835 0.62 -0.2 4,084,033 - RR42_RS18835 0.62 +0.3 4,084,214 + RR42_RS18835 0.86 +0.5 4,084,215 - RR42_RS18835 0.86 +0.8 4,084,215 - RR42_RS18835 0.86 -0.1 4,084,254 + -0.1 4,084,254 + -0.4 4,084,254 + -0.7 4,084,255 - -1.3 4,084,255 - -1.1 4,084,263 + -0.3 4,084,264 - +0.6 4,084,294 - -0.5 4,084,294 - -0.0 4,084,294 - +1.2 4,084,319 - -0.1 4,084,319 - +1.9 4,084,337 + -0.0 4,084,337 + +0.7 4,084,524 + RR42_RS18840 0.16 +0.0 4,084,713 + RR42_RS18840 0.31 +0.1 4,084,720 + RR42_RS18840 0.32 +0.1 4,084,877 + RR42_RS18840 0.45 -0.5 4,085,101 - RR42_RS18840 0.64 -0.0 4,085,101 - RR42_RS18840 0.64 +0.7 4,085,101 - RR42_RS18840 0.64 +1.2 4,085,370 - RR42_RS18840 0.86 -0.7 4,085,465 - +0.0 4,085,465 - +0.4 4,085,465 - -0.1 4,085,465 - -2.8 4,085,465 - -0.4 4,085,562 + +0.5 4,085,563 - +0.8 4,085,563 - -0.8 4,085,570 + +0.7 4,085,570 + +0.5 4,085,570 + -0.1 4,085,570 + -1.0 4,085,571 - -0.8 4,085,571 - -0.4 4,085,571 - -0.7 4,085,571 - +3.3 4,085,634 - -0.9 4,085,686 + -0.1 4,085,754 + +0.7 4,085,755 - +0.3 4,085,853 + -0.7 4,085,853 + +1.1 4,085,853 + -0.1 4,085,853 + -0.3 4,085,853 + +1.3 4,085,854 - +2.3 4,085,854 - -0.7 4,085,854 - -1.3 4,085,883 - +0.3 4,085,932 + +0.6 4,085,998 + -2.7 4,085,998 + -2.5 4,085,998 + -2.9 4,086,026 - -1.3
Or see this region's nucleotide sequence