Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18500 and RR42_RS18505 are separated by 38 nucleotides RR42_RS18505 and RR42_RS18510 are separated by 179 nucleotides RR42_RS18510 and RR42_RS18515 are separated by 102 nucleotides
RR42_RS18500: RR42_RS18500 - aldehyde dehydrogenase, at 4,003,206 to 4,004,684
_RS18500
RR42_RS18505: RR42_RS18505 - saccharopine dehydrogenase, at 4,004,723 to 4,005,928
_RS18505
RR42_RS18510: RR42_RS18510 - AsnC family transcriptional regulator, at 4,006,108 to 4,006,557
_RS18510
RR42_RS18515: RR42_RS18515 - dihydrodipicolinate reductase, at 4,006,660 to 4,007,469
_RS18515
Position (kb)
4004
4005
4006 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4003.797 kb on - strand, within RR42_RS18500 at 4003.797 kb on - strand, within RR42_RS18500 at 4004.284 kb on + strand, within RR42_RS18500 at 4004.285 kb on - strand, within RR42_RS18500 at 4004.336 kb on + strand, within RR42_RS18500 at 4004.337 kb on - strand, within RR42_RS18500 at 4004.407 kb on - strand, within RR42_RS18500 at 4004.565 kb on - strand at 4004.565 kb on - strand at 4004.565 kb on - strand at 4004.583 kb on + strand at 4004.757 kb on + strand at 4004.757 kb on + strand at 4004.758 kb on - strand at 4004.758 kb on - strand at 4005.153 kb on + strand, within RR42_RS18505 at 4005.153 kb on + strand, within RR42_RS18505 at 4005.215 kb on - strand, within RR42_RS18505 at 4005.217 kb on + strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.229 kb on - strand, within RR42_RS18505 at 4005.321 kb on + strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.360 kb on + strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.475 kb on + strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.967 kb on + strand at 4005.968 kb on - strand at 4005.968 kb on - strand at 4005.976 kb on + strand at 4005.976 kb on + strand at 4005.976 kb on + strand at 4005.977 kb on - strand at 4005.977 kb on - strand at 4005.977 kb on - strand at 4006.041 kb on + strand at 4006.042 kb on - strand at 4006.353 kb on - strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.418 kb on - strand, within RR42_RS18510 at 4006.418 kb on - strand, within RR42_RS18510 at 4006.581 kb on + strand at 4006.582 kb on - strand at 4006.582 kb on - strand at 4006.612 kb on + strand at 4006.612 kb on + strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.617 kb on - strand at 4006.617 kb on - strand at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.885 kb on + strand, within RR42_RS18515 at 4006.886 kb on - strand, within RR42_RS18515
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 4,003,797 - RR42_RS18500 0.40 -1.0 4,003,797 - RR42_RS18500 0.40 -0.3 4,004,284 + RR42_RS18500 0.73 -0.7 4,004,285 - RR42_RS18500 0.73 +0.9 4,004,336 + RR42_RS18500 0.76 +0.1 4,004,337 - RR42_RS18500 0.76 +0.3 4,004,407 - RR42_RS18500 0.81 +0.4 4,004,565 - -1.0 4,004,565 - -1.6 4,004,565 - -0.7 4,004,583 + +0.1 4,004,757 + +1.5 4,004,757 + -0.3 4,004,758 - -1.8 4,004,758 - -1.2 4,005,153 + RR42_RS18505 0.36 -0.6 4,005,153 + RR42_RS18505 0.36 -0.3 4,005,215 - RR42_RS18505 0.41 +1.3 4,005,217 + RR42_RS18505 0.41 +1.2 4,005,218 - RR42_RS18505 0.41 +1.0 4,005,218 - RR42_RS18505 0.41 -0.8 4,005,218 - RR42_RS18505 0.41 -0.2 4,005,229 - RR42_RS18505 0.42 +0.5 4,005,321 + RR42_RS18505 0.50 -0.2 4,005,322 - RR42_RS18505 0.50 -0.5 4,005,322 - RR42_RS18505 0.50 -0.5 4,005,322 - RR42_RS18505 0.50 +0.8 4,005,360 + RR42_RS18505 0.53 -1.9 4,005,361 - RR42_RS18505 0.53 -0.3 4,005,361 - RR42_RS18505 0.53 +0.7 4,005,361 - RR42_RS18505 0.53 -0.6 4,005,475 + RR42_RS18505 0.62 -0.7 4,005,580 - RR42_RS18505 0.71 -0.5 4,005,580 - RR42_RS18505 0.71 -0.4 4,005,580 - RR42_RS18505 0.71 +0.3 4,005,967 + -0.6 4,005,968 - -0.1 4,005,968 - +1.0 4,005,976 + -0.7 4,005,976 + +0.1 4,005,976 + -0.4 4,005,977 - +0.4 4,005,977 - -0.2 4,005,977 - +0.3 4,006,041 + +0.5 4,006,042 - +0.0 4,006,353 - RR42_RS18510 0.54 +1.8 4,006,417 + RR42_RS18510 0.69 -0.9 4,006,417 + RR42_RS18510 0.69 -0.3 4,006,417 + RR42_RS18510 0.69 +1.2 4,006,418 - RR42_RS18510 0.69 -1.8 4,006,418 - RR42_RS18510 0.69 +1.0 4,006,581 + -0.3 4,006,582 - +0.4 4,006,582 - -0.9 4,006,612 + -0.0 4,006,612 + -0.3 4,006,613 - -0.2 4,006,613 - -0.5 4,006,613 - -1.9 4,006,613 - +0.9 4,006,613 - +0.7 4,006,613 - +1.3 4,006,616 + +0.6 4,006,616 + +1.7 4,006,616 + -0.6 4,006,616 + +1.8 4,006,616 + +0.1 4,006,616 + +0.0 4,006,616 + +0.4 4,006,616 + +0.4 4,006,616 + -0.0 4,006,616 + -1.7 4,006,617 - +0.7 4,006,617 - +0.1 4,006,876 + RR42_RS18515 0.27 +1.2 4,006,876 + RR42_RS18515 0.27 -0.6 4,006,876 + RR42_RS18515 0.27 +0.4 4,006,876 + RR42_RS18515 0.27 +0.6 4,006,885 + RR42_RS18515 0.28 +1.5 4,006,886 - RR42_RS18515 0.28 +0.2
Or see this region's nucleotide sequence