Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17490 and RR42_RS17495 are separated by 99 nucleotides RR42_RS17495 and RR42_RS17500 are separated by 135 nucleotides RR42_RS17500 and RR42_RS17505 overlap by 4 nucleotides RR42_RS17505 and RR42_RS17510 are separated by 294 nucleotides
RR42_RS17490: RR42_RS17490 - NADP transhydrogenase subunit alpha, at 3,785,644 to 3,786,765
_RS17490
RR42_RS17495: RR42_RS17495 - hypothetical protein, at 3,786,865 to 3,787,047
_RS17495
RR42_RS17500: RR42_RS17500 - NAD(P) transhydrogenase, at 3,787,183 to 3,787,515
_RS17500
RR42_RS17505: RR42_RS17505 - NAD synthetase, at 3,787,512 to 3,788,972
_RS17505
RR42_RS17510: RR42_RS17510 - chemotaxis protein CheY, at 3,789,267 to 3,790,313
_RS17510
Position (kb)
3787
3788
3789 Strain fitness (log2 ratio)
-2
-1
0
1 at 3786.630 kb on - strand, within RR42_RS17490 at 3786.632 kb on - strand, within RR42_RS17490 at 3786.632 kb on - strand, within RR42_RS17490 at 3786.857 kb on - strand at 3786.857 kb on - strand at 3786.857 kb on - strand at 3786.889 kb on + strand, within RR42_RS17495 at 3786.889 kb on + strand, within RR42_RS17495 at 3786.889 kb on + strand, within RR42_RS17495 at 3786.889 kb on + strand, within RR42_RS17495 at 3786.890 kb on - strand, within RR42_RS17495 at 3787.022 kb on + strand, within RR42_RS17495 at 3787.051 kb on + strand at 3787.052 kb on - strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.055 kb on + strand at 3787.056 kb on - strand at 3787.056 kb on - strand at 3787.056 kb on - strand at 3787.056 kb on - strand at 3787.237 kb on + strand, within RR42_RS17500 at 3787.539 kb on + strand at 3787.776 kb on + strand, within RR42_RS17505 at 3787.777 kb on - strand, within RR42_RS17505 at 3787.777 kb on - strand, within RR42_RS17505 at 3788.022 kb on + strand, within RR42_RS17505 at 3788.023 kb on - strand, within RR42_RS17505 at 3788.346 kb on + strand, within RR42_RS17505 at 3788.346 kb on + strand, within RR42_RS17505 at 3788.346 kb on + strand, within RR42_RS17505 at 3788.347 kb on - strand, within RR42_RS17505 at 3788.347 kb on - strand, within RR42_RS17505 at 3788.347 kb on - strand, within RR42_RS17505 at 3788.347 kb on - strand, within RR42_RS17505 at 3788.604 kb on + strand, within RR42_RS17505 at 3788.605 kb on - strand, within RR42_RS17505 at 3788.605 kb on - strand, within RR42_RS17505 at 3788.605 kb on - strand, within RR42_RS17505 at 3788.676 kb on + strand, within RR42_RS17505 at 3788.823 kb on + strand, within RR42_RS17505 at 3788.823 kb on + strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.824 kb on - strand, within RR42_RS17505 at 3788.898 kb on + strand at 3788.899 kb on - strand at 3788.899 kb on - strand at 3788.971 kb on - strand at 3788.971 kb on - strand at 3788.971 kb on - strand at 3788.971 kb on - strand at 3788.971 kb on - strand at 3788.971 kb on - strand at 3789.091 kb on - strand at 3789.091 kb on - strand at 3789.091 kb on - strand at 3789.091 kb on - strand at 3789.091 kb on - strand at 3789.091 kb on - strand at 3789.092 kb on + strand at 3789.092 kb on + strand at 3789.092 kb on + strand at 3789.092 kb on + strand at 3789.093 kb on - strand at 3789.093 kb on - strand at 3789.093 kb on - strand at 3789.150 kb on - strand at 3789.341 kb on + strand at 3789.341 kb on + strand at 3789.442 kb on - strand, within RR42_RS17510 at 3789.442 kb on - strand, within RR42_RS17510 at 3789.561 kb on + strand, within RR42_RS17510 at 3789.561 kb on + strand, within RR42_RS17510 at 3789.735 kb on + strand, within RR42_RS17510 at 3789.758 kb on + strand, within RR42_RS17510 at 3789.758 kb on + strand, within RR42_RS17510 at 3789.759 kb on - strand, within RR42_RS17510 at 3789.759 kb on - strand, within RR42_RS17510 at 3789.759 kb on - strand, within RR42_RS17510 at 3789.804 kb on + strand, within RR42_RS17510 at 3789.928 kb on - strand, within RR42_RS17510 at 3789.928 kb on - strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.930 kb on + strand, within RR42_RS17510 at 3789.931 kb on - strand, within RR42_RS17510 at 3789.931 kb on - strand, within RR42_RS17510 at 3789.936 kb on + strand, within RR42_RS17510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,786,630 - RR42_RS17490 0.88 +0.2 3,786,632 - RR42_RS17490 0.88 +1.7 3,786,632 - RR42_RS17490 0.88 -0.1 3,786,857 - -0.3 3,786,857 - -2.6 3,786,857 - -0.5 3,786,889 + RR42_RS17495 0.13 +1.4 3,786,889 + RR42_RS17495 0.13 -0.6 3,786,889 + RR42_RS17495 0.13 +0.1 3,786,889 + RR42_RS17495 0.13 -0.8 3,786,890 - RR42_RS17495 0.14 -0.1 3,787,022 + RR42_RS17495 0.86 -1.4 3,787,051 + -0.1 3,787,052 - -0.0 3,787,055 + -1.6 3,787,055 + -0.1 3,787,055 + +0.1 3,787,055 + -2.4 3,787,055 + +0.2 3,787,055 + -0.4 3,787,055 + -0.6 3,787,055 + -1.0 3,787,055 + +0.2 3,787,055 + -0.2 3,787,055 + -1.4 3,787,055 + -0.3 3,787,056 - -0.4 3,787,056 - -1.1 3,787,056 - +0.4 3,787,056 - +0.0 3,787,237 + RR42_RS17500 0.16 +0.8 3,787,539 + -0.0 3,787,776 + RR42_RS17505 0.18 -0.2 3,787,777 - RR42_RS17505 0.18 -0.0 3,787,777 - RR42_RS17505 0.18 +0.8 3,788,022 + RR42_RS17505 0.35 +0.8 3,788,023 - RR42_RS17505 0.35 -0.8 3,788,346 + RR42_RS17505 0.57 -0.1 3,788,346 + RR42_RS17505 0.57 -0.4 3,788,346 + RR42_RS17505 0.57 +0.1 3,788,347 - RR42_RS17505 0.57 +0.6 3,788,347 - RR42_RS17505 0.57 -0.2 3,788,347 - RR42_RS17505 0.57 -0.6 3,788,347 - RR42_RS17505 0.57 +1.3 3,788,604 + RR42_RS17505 0.75 +0.6 3,788,605 - RR42_RS17505 0.75 -0.4 3,788,605 - RR42_RS17505 0.75 -2.2 3,788,605 - RR42_RS17505 0.75 -1.1 3,788,676 + RR42_RS17505 0.80 -0.9 3,788,823 + RR42_RS17505 0.90 +0.2 3,788,823 + RR42_RS17505 0.90 -0.4 3,788,824 - RR42_RS17505 0.90 -0.3 3,788,824 - RR42_RS17505 0.90 -0.7 3,788,824 - RR42_RS17505 0.90 -1.0 3,788,824 - RR42_RS17505 0.90 +0.2 3,788,824 - RR42_RS17505 0.90 -2.5 3,788,824 - RR42_RS17505 0.90 -1.6 3,788,898 + +0.2 3,788,899 - -0.9 3,788,899 - -0.1 3,788,971 - -1.5 3,788,971 - -0.1 3,788,971 - -0.3 3,788,971 - +0.5 3,788,971 - -0.0 3,788,971 - -0.4 3,789,091 - -0.2 3,789,091 - +0.3 3,789,091 - -0.6 3,789,091 - +0.1 3,789,091 - +0.1 3,789,091 - +0.2 3,789,092 + -0.0 3,789,092 + -0.1 3,789,092 + -0.2 3,789,092 + -0.3 3,789,093 - +0.4 3,789,093 - -0.3 3,789,093 - +1.0 3,789,150 - +0.1 3,789,341 + +0.8 3,789,341 + +0.8 3,789,442 - RR42_RS17510 0.17 +0.2 3,789,442 - RR42_RS17510 0.17 -0.5 3,789,561 + RR42_RS17510 0.28 +1.1 3,789,561 + RR42_RS17510 0.28 +0.1 3,789,735 + RR42_RS17510 0.45 +0.6 3,789,758 + RR42_RS17510 0.47 +0.9 3,789,758 + RR42_RS17510 0.47 +0.7 3,789,759 - RR42_RS17510 0.47 +0.5 3,789,759 - RR42_RS17510 0.47 +0.5 3,789,759 - RR42_RS17510 0.47 -1.6 3,789,804 + RR42_RS17510 0.51 +0.7 3,789,928 - RR42_RS17510 0.63 -0.7 3,789,928 - RR42_RS17510 0.63 +1.0 3,789,930 + RR42_RS17510 0.63 +0.3 3,789,930 + RR42_RS17510 0.63 +0.8 3,789,930 + RR42_RS17510 0.63 +0.9 3,789,930 + RR42_RS17510 0.63 +0.9 3,789,930 + RR42_RS17510 0.63 +1.4 3,789,930 + RR42_RS17510 0.63 -0.4 3,789,931 - RR42_RS17510 0.63 -1.0 3,789,931 - RR42_RS17510 0.63 +0.5 3,789,936 + RR42_RS17510 0.64 +0.2
Or see this region's nucleotide sequence