Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16425 and RR42_RS16430 are separated by 98 nucleotides RR42_RS16430 and RR42_RS16435 are separated by 477 nucleotides RR42_RS16435 and RR42_RS16440 are separated by 52 nucleotides
RR42_RS16425: RR42_RS16425 - SAM-dependent methyltransferase, at 3,555,735 to 3,556,598
_RS16425
RR42_RS16430: RR42_RS16430 - transposase, at 3,556,697 to 3,557,038
_RS16430
RR42_RS16435: RR42_RS16435 - hypothetical protein, at 3,557,516 to 3,558,520
_RS16435
RR42_RS16440: RR42_RS16440 - dihydropyrimidinase, at 3,558,573 to 3,560,009
_RS16440
Position (kb)
3557
3558
3559 Strain fitness (log2 ratio)
-1
0
1 at 3556.606 kb on + strand at 3556.737 kb on + strand, within RR42_RS16430 at 3556.926 kb on + strand, within RR42_RS16430 at 3556.927 kb on - strand, within RR42_RS16430 at 3556.927 kb on - strand, within RR42_RS16430 at 3556.927 kb on - strand, within RR42_RS16430 at 3556.927 kb on - strand, within RR42_RS16430 at 3557.081 kb on + strand at 3557.082 kb on - strand at 3557.142 kb on - strand at 3557.254 kb on - strand at 3557.268 kb on + strand at 3557.359 kb on + strand at 3557.369 kb on + strand at 3557.370 kb on - strand at 3557.712 kb on + strand, within RR42_RS16435 at 3557.716 kb on + strand, within RR42_RS16435 at 3557.716 kb on + strand, within RR42_RS16435 at 3557.727 kb on + strand, within RR42_RS16435 at 3557.935 kb on + strand, within RR42_RS16435 at 3557.935 kb on + strand, within RR42_RS16435 at 3558.002 kb on + strand, within RR42_RS16435 at 3558.058 kb on - strand, within RR42_RS16435 at 3558.073 kb on - strand, within RR42_RS16435 at 3558.142 kb on - strand, within RR42_RS16435 at 3558.239 kb on + strand, within RR42_RS16435 at 3558.620 kb on + strand at 3558.620 kb on + strand at 3558.723 kb on + strand, within RR42_RS16440 at 3558.723 kb on + strand, within RR42_RS16440 at 3558.785 kb on + strand, within RR42_RS16440 at 3558.785 kb on + strand, within RR42_RS16440 at 3558.785 kb on + strand, within RR42_RS16440 at 3558.786 kb on - strand, within RR42_RS16440 at 3558.786 kb on - strand, within RR42_RS16440 at 3559.105 kb on - strand, within RR42_RS16440 at 3559.150 kb on + strand, within RR42_RS16440 at 3559.150 kb on + strand, within RR42_RS16440 at 3559.150 kb on + strand, within RR42_RS16440 at 3559.284 kb on + strand, within RR42_RS16440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,556,606 + +0.7 3,556,737 + RR42_RS16430 0.12 +0.2 3,556,926 + RR42_RS16430 0.67 +1.1 3,556,927 - RR42_RS16430 0.67 -0.9 3,556,927 - RR42_RS16430 0.67 -0.9 3,556,927 - RR42_RS16430 0.67 +0.2 3,556,927 - RR42_RS16430 0.67 -1.2 3,557,081 + -1.1 3,557,082 - +0.4 3,557,142 - -0.5 3,557,254 - +0.0 3,557,268 + -0.4 3,557,359 + +0.8 3,557,369 + +0.9 3,557,370 - +0.0 3,557,712 + RR42_RS16435 0.20 +0.2 3,557,716 + RR42_RS16435 0.20 +1.3 3,557,716 + RR42_RS16435 0.20 -1.4 3,557,727 + RR42_RS16435 0.21 -0.3 3,557,935 + RR42_RS16435 0.42 +0.4 3,557,935 + RR42_RS16435 0.42 -1.3 3,558,002 + RR42_RS16435 0.48 +0.4 3,558,058 - RR42_RS16435 0.54 +0.3 3,558,073 - RR42_RS16435 0.55 +0.7 3,558,142 - RR42_RS16435 0.62 -0.4 3,558,239 + RR42_RS16435 0.72 -1.2 3,558,620 + +0.6 3,558,620 + +1.6 3,558,723 + RR42_RS16440 0.10 +0.5 3,558,723 + RR42_RS16440 0.10 -0.2 3,558,785 + RR42_RS16440 0.15 -0.4 3,558,785 + RR42_RS16440 0.15 -1.2 3,558,785 + RR42_RS16440 0.15 +0.4 3,558,786 - RR42_RS16440 0.15 -0.0 3,558,786 - RR42_RS16440 0.15 -0.5 3,559,105 - RR42_RS16440 0.37 -0.3 3,559,150 + RR42_RS16440 0.40 -1.5 3,559,150 + RR42_RS16440 0.40 +0.7 3,559,150 + RR42_RS16440 0.40 +0.3 3,559,284 + RR42_RS16440 0.49 +0.1
Or see this region's nucleotide sequence