Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16235 and RR42_RS16240 are separated by 44 nucleotides RR42_RS16240 and RR42_RS16245 are separated by 190 nucleotides
RR42_RS16235: RR42_RS16235 - permease, at 3,514,856 to 3,516,010
_RS16235
RR42_RS16240: RR42_RS16240 - cobalamin biosynthesis protein CbiX, at 3,516,055 to 3,516,459
_RS16240
RR42_RS16245: RR42_RS16245 - uroporphyrin-III methyltransferase, at 3,516,650 to 3,517,420
_RS16245
Position (kb)
3516
3517 Strain fitness (log2 ratio)
-2
-1
0
1 at 3516.009 kb on - strand at 3516.009 kb on - strand at 3516.060 kb on + strand at 3516.060 kb on + strand at 3516.061 kb on - strand at 3516.418 kb on + strand, within RR42_RS16240 at 3516.418 kb on + strand, within RR42_RS16240 at 3516.614 kb on + strand at 3516.615 kb on - strand at 3516.632 kb on + strand at 3516.633 kb on - strand at 3516.633 kb on - strand at 3516.672 kb on + strand at 3516.673 kb on - strand at 3516.673 kb on - strand at 3516.673 kb on - strand at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.933 kb on + strand, within RR42_RS16245 at 3516.933 kb on + strand, within RR42_RS16245 at 3516.944 kb on - strand, within RR42_RS16245 at 3517.096 kb on - strand, within RR42_RS16245 at 3517.370 kb on - strand at 3517.407 kb on + strand at 3517.456 kb on + strand at 3517.456 kb on + strand at 3517.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,516,009 - -0.1 3,516,009 - -1.5 3,516,060 + -0.3 3,516,060 + +1.0 3,516,061 - -0.8 3,516,418 + RR42_RS16240 0.90 -1.0 3,516,418 + RR42_RS16240 0.90 -2.7 3,516,614 + +0.2 3,516,615 - -0.3 3,516,632 + +0.3 3,516,633 - -1.9 3,516,633 - +1.3 3,516,672 + -0.8 3,516,673 - +0.7 3,516,673 - +0.9 3,516,673 - -1.7 3,516,930 + RR42_RS16245 0.36 +0.6 3,516,930 + RR42_RS16245 0.36 -1.4 3,516,930 + RR42_RS16245 0.36 -0.3 3,516,930 + RR42_RS16245 0.36 -1.7 3,516,931 - RR42_RS16245 0.36 +0.1 3,516,931 - RR42_RS16245 0.36 +0.7 3,516,931 - RR42_RS16245 0.36 +0.7 3,516,933 + RR42_RS16245 0.37 +0.9 3,516,933 + RR42_RS16245 0.37 -0.9 3,516,944 - RR42_RS16245 0.38 -1.3 3,517,096 - RR42_RS16245 0.58 +0.1 3,517,370 - +1.3 3,517,407 + +0.2 3,517,456 + +0.0 3,517,456 + +0.9 3,517,456 + +1.4
Or see this region's nucleotide sequence