Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15845 and RR42_RS15850 are separated by 5 nucleotides RR42_RS15850 and RR42_RS15855 are separated by 64 nucleotides
RR42_RS15845: RR42_RS15845 - biotin transporter BioY, at 3,425,722 to 3,426,558
_RS15845
RR42_RS15850: RR42_RS15850 - glycerol acyltransferase, at 3,426,564 to 3,427,406
_RS15850
RR42_RS15855: RR42_RS15855 - dihydroorotase, at 3,427,471 to 3,428,745
_RS15855
Position (kb)
3426
3427
3428 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3425.574 kb on - strand at 3425.579 kb on - strand at 3425.579 kb on - strand at 3425.601 kb on - strand at 3425.624 kb on - strand at 3425.634 kb on - strand at 3425.634 kb on - strand at 3425.634 kb on - strand at 3425.647 kb on + strand at 3425.661 kb on + strand at 3425.661 kb on + strand at 3425.661 kb on + strand at 3425.661 kb on + strand at 3425.661 kb on + strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.662 kb on - strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.707 kb on + strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.708 kb on - strand at 3425.792 kb on + strand at 3425.792 kb on + strand at 3425.793 kb on - strand at 3425.829 kb on - strand, within RR42_RS15845 at 3426.291 kb on - strand, within RR42_RS15845 at 3426.447 kb on + strand, within RR42_RS15845 at 3426.448 kb on - strand, within RR42_RS15845 at 3426.765 kb on + strand, within RR42_RS15850 at 3426.765 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.833 kb on + strand, within RR42_RS15850 at 3426.834 kb on - strand, within RR42_RS15850 at 3426.834 kb on - strand, within RR42_RS15850 at 3426.834 kb on - strand, within RR42_RS15850 at 3426.834 kb on - strand, within RR42_RS15850 at 3426.834 kb on - strand, within RR42_RS15850 at 3426.835 kb on + strand, within RR42_RS15850 at 3426.835 kb on + strand, within RR42_RS15850 at 3426.835 kb on + strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.836 kb on - strand, within RR42_RS15850 at 3426.877 kb on + strand, within RR42_RS15850 at 3426.877 kb on + strand, within RR42_RS15850 at 3426.877 kb on + strand, within RR42_RS15850 at 3426.877 kb on + strand, within RR42_RS15850 at 3427.022 kb on + strand, within RR42_RS15850 at 3427.023 kb on - strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.171 kb on + strand, within RR42_RS15850 at 3427.172 kb on - strand, within RR42_RS15850 at 3427.172 kb on - strand, within RR42_RS15850 at 3427.172 kb on - strand, within RR42_RS15850 at 3427.172 kb on - strand, within RR42_RS15850 at 3427.302 kb on - strand, within RR42_RS15850 at 3427.427 kb on + strand at 3427.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,425,574 - -2.0 3,425,579 - -1.7 3,425,579 - -1.7 3,425,601 - +0.5 3,425,624 - -2.3 3,425,634 - -0.8 3,425,634 - -1.8 3,425,634 - +1.1 3,425,647 + +1.5 3,425,661 + +1.6 3,425,661 + +1.8 3,425,661 + +1.5 3,425,661 + +1.2 3,425,661 + +0.8 3,425,662 - -2.0 3,425,662 - +0.5 3,425,662 - -1.5 3,425,662 - -1.7 3,425,662 - -1.7 3,425,662 - -2.2 3,425,662 - -1.0 3,425,707 + +1.7 3,425,707 + +1.5 3,425,707 + +3.2 3,425,707 + +1.7 3,425,707 + -0.2 3,425,707 + +1.8 3,425,707 + +0.1 3,425,707 + +2.0 3,425,707 + +1.4 3,425,708 - -2.0 3,425,708 - -2.1 3,425,708 - -1.5 3,425,708 - -2.1 3,425,708 - -1.8 3,425,708 - -1.1 3,425,708 - -0.8 3,425,792 + +0.1 3,425,792 + +1.4 3,425,793 - -0.9 3,425,829 - RR42_RS15845 0.13 -1.1 3,426,291 - RR42_RS15845 0.68 -1.7 3,426,447 + RR42_RS15845 0.87 +2.1 3,426,448 - RR42_RS15845 0.87 +0.9 3,426,765 + RR42_RS15850 0.24 +1.8 3,426,765 + RR42_RS15850 0.24 +2.4 3,426,833 + RR42_RS15850 0.32 +1.5 3,426,833 + RR42_RS15850 0.32 +2.7 3,426,833 + RR42_RS15850 0.32 +1.7 3,426,833 + RR42_RS15850 0.32 +2.2 3,426,833 + RR42_RS15850 0.32 +1.7 3,426,833 + RR42_RS15850 0.32 +2.3 3,426,834 - RR42_RS15850 0.32 +0.7 3,426,834 - RR42_RS15850 0.32 +1.4 3,426,834 - RR42_RS15850 0.32 +2.2 3,426,834 - RR42_RS15850 0.32 +1.9 3,426,834 - RR42_RS15850 0.32 +2.8 3,426,835 + RR42_RS15850 0.32 +1.8 3,426,835 + RR42_RS15850 0.32 +0.4 3,426,835 + RR42_RS15850 0.32 +1.7 3,426,836 - RR42_RS15850 0.32 +1.1 3,426,836 - RR42_RS15850 0.32 +1.8 3,426,836 - RR42_RS15850 0.32 +1.9 3,426,836 - RR42_RS15850 0.32 +1.1 3,426,836 - RR42_RS15850 0.32 +1.4 3,426,836 - RR42_RS15850 0.32 +1.7 3,426,836 - RR42_RS15850 0.32 +2.7 3,426,836 - RR42_RS15850 0.32 +2.0 3,426,877 + RR42_RS15850 0.37 +2.1 3,426,877 + RR42_RS15850 0.37 +2.6 3,426,877 + RR42_RS15850 0.37 +1.4 3,426,877 + RR42_RS15850 0.37 +2.2 3,427,022 + RR42_RS15850 0.54 +3.1 3,427,023 - RR42_RS15850 0.54 +1.8 3,427,171 + RR42_RS15850 0.72 +1.9 3,427,171 + RR42_RS15850 0.72 +2.0 3,427,171 + RR42_RS15850 0.72 +2.5 3,427,171 + RR42_RS15850 0.72 +1.8 3,427,171 + RR42_RS15850 0.72 +3.2 3,427,171 + RR42_RS15850 0.72 +2.4 3,427,171 + RR42_RS15850 0.72 +1.6 3,427,172 - RR42_RS15850 0.72 +2.0 3,427,172 - RR42_RS15850 0.72 +1.2 3,427,172 - RR42_RS15850 0.72 +0.8 3,427,172 - RR42_RS15850 0.72 +0.3 3,427,302 - RR42_RS15850 0.88 +2.5 3,427,427 + +0.8 3,427,484 + +2.5
Or see this region's nucleotide sequence