Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15590 and RR42_RS15595 are separated by 82 nucleotides RR42_RS15595 and RR42_RS15600 are separated by 76 nucleotides
RR42_RS15590: RR42_RS15590 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,366,920 to 3,368,449
_RS15590
RR42_RS15595: RR42_RS15595 - acetoacetyl-CoA synthetase, at 3,368,532 to 3,370,568
_RS15595
RR42_RS15600: RR42_RS15600 - glutathione S-transferase, at 3,370,645 to 3,371,301
_RS15600
Position (kb)
3368
3369
3370
3371 Strain fitness (log2 ratio)
-2
-1
0
1 at 3368.464 kb on + strand at 3368.464 kb on + strand at 3368.465 kb on - strand at 3368.715 kb on + strand at 3368.808 kb on + strand, within RR42_RS15595 at 3368.808 kb on + strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.992 kb on - strand, within RR42_RS15595 at 3369.473 kb on + strand, within RR42_RS15595 at 3369.504 kb on + strand, within RR42_RS15595 at 3369.504 kb on + strand, within RR42_RS15595 at 3369.505 kb on - strand, within RR42_RS15595 at 3369.577 kb on - strand, within RR42_RS15595 at 3369.719 kb on - strand, within RR42_RS15595 at 3369.771 kb on + strand, within RR42_RS15595 at 3369.771 kb on + strand, within RR42_RS15595 at 3369.772 kb on - strand, within RR42_RS15595 at 3369.879 kb on + strand, within RR42_RS15595 at 3370.122 kb on + strand, within RR42_RS15595 at 3370.123 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.257 kb on + strand, within RR42_RS15595 at 3370.257 kb on + strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.259 kb on + strand, within RR42_RS15595 at 3370.260 kb on - strand, within RR42_RS15595 at 3370.260 kb on - strand, within RR42_RS15595 at 3370.509 kb on + strand at 3370.510 kb on - strand at 3370.742 kb on + strand, within RR42_RS15600 at 3370.775 kb on + strand, within RR42_RS15600 at 3370.775 kb on + strand, within RR42_RS15600 at 3370.776 kb on - strand, within RR42_RS15600 at 3370.776 kb on - strand, within RR42_RS15600 at 3370.806 kb on - strand, within RR42_RS15600 at 3370.806 kb on - strand, within RR42_RS15600 at 3370.845 kb on - strand, within RR42_RS15600 at 3371.079 kb on - strand, within RR42_RS15600 at 3371.272 kb on - strand at 3371.435 kb on + strand at 3371.436 kb on - strand at 3371.449 kb on - strand at 3371.451 kb on + strand at 3371.451 kb on + strand at 3371.451 kb on + strand at 3371.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,368,464 + +1.3 3,368,464 + +0.5 3,368,465 - +0.8 3,368,715 + +0.1 3,368,808 + RR42_RS15595 0.14 -2.2 3,368,808 + RR42_RS15595 0.14 -1.4 3,368,809 - RR42_RS15595 0.14 -0.3 3,368,809 - RR42_RS15595 0.14 -0.6 3,368,809 - RR42_RS15595 0.14 -2.5 3,368,992 - RR42_RS15595 0.23 -1.9 3,369,473 + RR42_RS15595 0.46 -0.8 3,369,504 + RR42_RS15595 0.48 +0.8 3,369,504 + RR42_RS15595 0.48 +1.7 3,369,505 - RR42_RS15595 0.48 +1.1 3,369,577 - RR42_RS15595 0.51 -1.6 3,369,719 - RR42_RS15595 0.58 -0.2 3,369,771 + RR42_RS15595 0.61 +0.6 3,369,771 + RR42_RS15595 0.61 -0.9 3,369,772 - RR42_RS15595 0.61 +0.3 3,369,879 + RR42_RS15595 0.66 -1.1 3,370,122 + RR42_RS15595 0.78 +0.1 3,370,123 - RR42_RS15595 0.78 -1.3 3,370,240 - RR42_RS15595 0.84 -0.2 3,370,240 - RR42_RS15595 0.84 -1.5 3,370,240 - RR42_RS15595 0.84 -1.2 3,370,257 + RR42_RS15595 0.85 +0.4 3,370,257 + RR42_RS15595 0.85 +0.4 3,370,258 - RR42_RS15595 0.85 +1.7 3,370,258 - RR42_RS15595 0.85 -0.1 3,370,258 - RR42_RS15595 0.85 +0.1 3,370,258 - RR42_RS15595 0.85 -1.2 3,370,259 + RR42_RS15595 0.85 +0.4 3,370,260 - RR42_RS15595 0.85 -0.5 3,370,260 - RR42_RS15595 0.85 -0.1 3,370,509 + +1.0 3,370,510 - +0.0 3,370,742 + RR42_RS15600 0.15 +0.4 3,370,775 + RR42_RS15600 0.20 +0.2 3,370,775 + RR42_RS15600 0.20 -0.3 3,370,776 - RR42_RS15600 0.20 +1.1 3,370,776 - RR42_RS15600 0.20 -1.6 3,370,806 - RR42_RS15600 0.25 +0.1 3,370,806 - RR42_RS15600 0.25 -1.0 3,370,845 - RR42_RS15600 0.30 -1.1 3,371,079 - RR42_RS15600 0.66 +0.0 3,371,272 - +0.9 3,371,435 + +1.0 3,371,436 - -0.7 3,371,449 - +0.9 3,371,451 + -0.9 3,371,451 + +0.6 3,371,451 + -0.5 3,371,497 + +0.8
Or see this region's nucleotide sequence