Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15440 and RR42_RS15445 are separated by 65 nucleotides RR42_RS15445 and RR42_RS15450 are separated by 153 nucleotides RR42_RS15450 and RR42_RS15455 are separated by 18 nucleotides RR42_RS15455 and RR42_RS15460 are separated by 26 nucleotides
RR42_RS15440: RR42_RS15440 - transporter, at 3,336,696 to 3,337,811
_RS15440
RR42_RS15445: RR42_RS15445 - 7-cyano-7-deazaguanine synthase, at 3,337,877 to 3,338,560
_RS15445
RR42_RS15450: RR42_RS15450 - Tol system periplasmic component YbgF, at 3,338,714 to 3,339,472
_RS15450
RR42_RS15455: RR42_RS15455 - membrane protein, at 3,339,491 to 3,339,997
_RS15455
RR42_RS15460: RR42_RS15460 - translocation protein TolB, at 3,340,024 to 3,341,352
_RS15460
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3337.773 kb on + strand at 3337.774 kb on - strand at 3337.774 kb on - strand at 3337.774 kb on - strand at 3337.797 kb on - strand at 3337.894 kb on - strand at 3337.991 kb on - strand, within RR42_RS15445 at 3337.991 kb on - strand, within RR42_RS15445 at 3337.991 kb on - strand, within RR42_RS15445 at 3337.992 kb on + strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3338.013 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.152 kb on - strand, within RR42_RS15445 at 3338.152 kb on - strand, within RR42_RS15445 at 3338.181 kb on + strand, within RR42_RS15445 at 3338.264 kb on - strand, within RR42_RS15445 at 3338.281 kb on - strand, within RR42_RS15445 at 3338.284 kb on + strand, within RR42_RS15445 at 3338.634 kb on + strand at 3338.635 kb on - strand at 3338.659 kb on - strand at 3338.659 kb on - strand at 3338.663 kb on + strand at 3338.664 kb on - strand at 3338.664 kb on - strand at 3338.673 kb on + strand at 3338.673 kb on + strand at 3338.674 kb on - strand at 3338.674 kb on - strand at 3338.715 kb on + strand at 3338.715 kb on + strand at 3338.716 kb on - strand at 3338.769 kb on + strand at 3338.769 kb on + strand at 3338.778 kb on - strand at 3338.778 kb on - strand at 3338.880 kb on + strand, within RR42_RS15450 at 3338.881 kb on - strand, within RR42_RS15450 at 3338.922 kb on + strand, within RR42_RS15450 at 3338.976 kb on + strand, within RR42_RS15450 at 3338.977 kb on - strand, within RR42_RS15450 at 3339.069 kb on + strand, within RR42_RS15450 at 3339.070 kb on - strand, within RR42_RS15450 at 3339.166 kb on - strand, within RR42_RS15450 at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.492 kb on + strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.663 kb on + strand, within RR42_RS15455 at 3339.762 kb on + strand, within RR42_RS15455 at 3339.780 kb on + strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.798 kb on + strand, within RR42_RS15455 at 3339.799 kb on - strand, within RR42_RS15455 at 3339.892 kb on + strand, within RR42_RS15455 at 3339.997 kb on + strand at 3340.011 kb on - strand at 3340.026 kb on - strand at 3340.026 kb on - strand at 3340.319 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.419 kb on + strand, within RR42_RS15460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,337,773 + +0.4 3,337,774 - +0.5 3,337,774 - +0.9 3,337,774 - -0.5 3,337,797 - -0.6 3,337,894 - +0.5 3,337,991 - RR42_RS15445 0.17 -1.6 3,337,991 - RR42_RS15445 0.17 -0.1 3,337,991 - RR42_RS15445 0.17 -1.0 3,337,992 + RR42_RS15445 0.17 -1.9 3,337,993 - RR42_RS15445 0.17 +2.4 3,337,993 - RR42_RS15445 0.17 -3.6 3,337,993 - RR42_RS15445 0.17 -0.5 3,338,013 + RR42_RS15445 0.20 +0.7 3,338,151 + RR42_RS15445 0.40 -0.5 3,338,151 + RR42_RS15445 0.40 -1.2 3,338,151 + RR42_RS15445 0.40 -1.3 3,338,152 - RR42_RS15445 0.40 -0.7 3,338,152 - RR42_RS15445 0.40 -1.2 3,338,181 + RR42_RS15445 0.44 -1.6 3,338,264 - RR42_RS15445 0.57 -1.7 3,338,281 - RR42_RS15445 0.59 -2.6 3,338,284 + RR42_RS15445 0.60 -2.1 3,338,634 + -0.4 3,338,635 - +0.7 3,338,659 - -0.7 3,338,659 - +1.1 3,338,663 + +0.4 3,338,664 - +0.4 3,338,664 - +1.8 3,338,673 + -1.5 3,338,673 + +0.4 3,338,674 - +2.7 3,338,674 - -0.4 3,338,715 + -0.5 3,338,715 + -0.3 3,338,716 - +0.4 3,338,769 + +1.0 3,338,769 + +0.6 3,338,778 - -0.5 3,338,778 - +0.4 3,338,880 + RR42_RS15450 0.22 -0.2 3,338,881 - RR42_RS15450 0.22 -1.0 3,338,922 + RR42_RS15450 0.27 +0.1 3,338,976 + RR42_RS15450 0.35 +1.3 3,338,977 - RR42_RS15450 0.35 +2.1 3,339,069 + RR42_RS15450 0.47 +0.7 3,339,070 - RR42_RS15450 0.47 +0.9 3,339,166 - RR42_RS15450 0.60 +0.7 3,339,439 + -0.3 3,339,439 + -4.1 3,339,439 + -2.0 3,339,492 + -1.7 3,339,493 - +0.0 3,339,493 - -2.0 3,339,493 - -0.5 3,339,495 + -1.4 3,339,495 + -2.1 3,339,495 + +0.4 3,339,496 - -0.9 3,339,496 - -0.8 3,339,496 - +0.4 3,339,496 - -1.4 3,339,663 + RR42_RS15455 0.34 -1.2 3,339,762 + RR42_RS15455 0.53 -3.6 3,339,780 + RR42_RS15455 0.57 -0.8 3,339,781 - RR42_RS15455 0.57 -1.0 3,339,781 - RR42_RS15455 0.57 -1.7 3,339,781 - RR42_RS15455 0.57 -2.3 3,339,781 - RR42_RS15455 0.57 +2.2 3,339,781 - RR42_RS15455 0.57 -2.2 3,339,798 + RR42_RS15455 0.61 -0.7 3,339,799 - RR42_RS15455 0.61 -2.2 3,339,892 + RR42_RS15455 0.79 -0.5 3,339,997 + -1.9 3,340,011 - +0.4 3,340,026 - +0.6 3,340,026 - +0.1 3,340,319 + RR42_RS15460 0.22 -0.9 3,340,418 + RR42_RS15460 0.30 -0.9 3,340,418 + RR42_RS15460 0.30 -3.3 3,340,418 + RR42_RS15460 0.30 -0.9 3,340,419 + RR42_RS15460 0.30 -1.7
Or see this region's nucleotide sequence