Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14490 and RR42_RS14495 are separated by 552 nucleotides RR42_RS14495 and RR42_RS14500 are separated by 172 nucleotides
RR42_RS14490: RR42_RS14490 - aconitate hydratase, at 3,116,272 to 3,118,977
_RS14490
RR42_RS14495: RR42_RS14495 - hypothetical protein, at 3,119,530 to 3,120,732
_RS14495
RR42_RS14500: RR42_RS14500 - SAM-dependent methyltransferase, at 3,120,905 to 3,121,588
_RS14500
Position (kb)
3119
3120
3121 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3118.546 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.564 kb on + strand, within RR42_RS14490 at 3118.565 kb on - strand, within RR42_RS14490 at 3118.565 kb on - strand, within RR42_RS14490 at 3118.565 kb on - strand, within RR42_RS14490 at 3118.565 kb on - strand, within RR42_RS14490 at 3118.565 kb on - strand, within RR42_RS14490 at 3118.610 kb on - strand, within RR42_RS14490 at 3118.921 kb on + strand at 3118.921 kb on + strand at 3118.924 kb on + strand at 3118.925 kb on - strand at 3119.143 kb on - strand at 3119.299 kb on + strand at 3119.299 kb on + strand at 3119.299 kb on + strand at 3119.299 kb on + strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.300 kb on - strand at 3119.301 kb on + strand at 3119.301 kb on + strand at 3119.301 kb on + strand at 3119.301 kb on + strand at 3119.301 kb on + strand at 3119.301 kb on + strand at 3119.302 kb on - strand at 3119.302 kb on - strand at 3119.302 kb on - strand at 3119.302 kb on - strand at 3119.302 kb on - strand at 3119.302 kb on - strand at 3119.445 kb on + strand at 3119.446 kb on - strand at 3119.491 kb on + strand at 3119.491 kb on + strand at 3119.491 kb on + strand at 3119.497 kb on - strand at 3119.530 kb on - strand at 3119.816 kb on - strand, within RR42_RS14495 at 3119.816 kb on - strand, within RR42_RS14495 at 3119.965 kb on + strand, within RR42_RS14495 at 3119.971 kb on + strand, within RR42_RS14495 at 3119.971 kb on + strand, within RR42_RS14495 at 3119.972 kb on - strand, within RR42_RS14495 at 3119.972 kb on - strand, within RR42_RS14495 at 3120.022 kb on + strand, within RR42_RS14495 at 3120.022 kb on + strand, within RR42_RS14495 at 3120.023 kb on - strand, within RR42_RS14495 at 3120.359 kb on - strand, within RR42_RS14495 at 3120.359 kb on - strand, within RR42_RS14495 at 3120.439 kb on + strand, within RR42_RS14495 at 3120.440 kb on - strand, within RR42_RS14495 at 3120.447 kb on - strand, within RR42_RS14495 at 3120.482 kb on - strand, within RR42_RS14495 at 3120.502 kb on + strand, within RR42_RS14495 at 3120.731 kb on - strand at 3120.731 kb on - strand at 3120.960 kb on + strand at 3120.960 kb on + strand at 3121.058 kb on - strand, within RR42_RS14500 at 3121.058 kb on - strand, within RR42_RS14500 at 3121.058 kb on - strand, within RR42_RS14500 at 3121.059 kb on + strand, within RR42_RS14500 at 3121.059 kb on + strand, within RR42_RS14500 at 3121.060 kb on - strand, within RR42_RS14500 at 3121.060 kb on - strand, within RR42_RS14500 at 3121.090 kb on - strand, within RR42_RS14500 at 3121.176 kb on + strand, within RR42_RS14500 at 3121.185 kb on + strand, within RR42_RS14500 at 3121.185 kb on + strand, within RR42_RS14500 at 3121.186 kb on - strand, within RR42_RS14500 at 3121.591 kb on + strand at 3121.676 kb on + strand at 3121.677 kb on - strand at 3121.677 kb on - strand at 3121.682 kb on + strand at 3121.682 kb on + strand at 3121.683 kb on - strand at 3121.683 kb on - strand at 3121.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,118,546 + RR42_RS14490 0.84 -0.2 3,118,564 + RR42_RS14490 0.85 +2.0 3,118,564 + RR42_RS14490 0.85 +1.2 3,118,564 + RR42_RS14490 0.85 -1.0 3,118,564 + RR42_RS14490 0.85 +0.4 3,118,564 + RR42_RS14490 0.85 +0.2 3,118,564 + RR42_RS14490 0.85 -0.1 3,118,565 - RR42_RS14490 0.85 +0.4 3,118,565 - RR42_RS14490 0.85 -0.1 3,118,565 - RR42_RS14490 0.85 -2.3 3,118,565 - RR42_RS14490 0.85 -0.6 3,118,565 - RR42_RS14490 0.85 -1.1 3,118,610 - RR42_RS14490 0.86 +0.5 3,118,921 + +0.0 3,118,921 + +1.1 3,118,924 + -2.0 3,118,925 - -0.4 3,119,143 - +1.0 3,119,299 + -0.4 3,119,299 + -0.2 3,119,299 + -0.4 3,119,299 + +1.0 3,119,300 - +0.6 3,119,300 - -0.7 3,119,300 - -3.5 3,119,300 - -0.8 3,119,300 - -0.2 3,119,300 - -1.1 3,119,300 - -1.3 3,119,300 - -1.1 3,119,301 + -1.6 3,119,301 + +1.4 3,119,301 + +0.4 3,119,301 + -0.5 3,119,301 + -0.2 3,119,301 + +0.4 3,119,302 - -0.2 3,119,302 - +1.1 3,119,302 - -0.4 3,119,302 - +1.4 3,119,302 - -0.4 3,119,302 - -0.3 3,119,445 + +0.4 3,119,446 - -1.5 3,119,491 + -0.2 3,119,491 + +0.6 3,119,491 + +0.0 3,119,497 - -1.7 3,119,530 - -3.0 3,119,816 - RR42_RS14495 0.24 -1.6 3,119,816 - RR42_RS14495 0.24 -1.5 3,119,965 + RR42_RS14495 0.36 -4.0 3,119,971 + RR42_RS14495 0.37 -1.3 3,119,971 + RR42_RS14495 0.37 -1.9 3,119,972 - RR42_RS14495 0.37 -1.6 3,119,972 - RR42_RS14495 0.37 -1.1 3,120,022 + RR42_RS14495 0.41 -0.6 3,120,022 + RR42_RS14495 0.41 -0.9 3,120,023 - RR42_RS14495 0.41 +1.6 3,120,359 - RR42_RS14495 0.69 -1.4 3,120,359 - RR42_RS14495 0.69 -1.0 3,120,439 + RR42_RS14495 0.76 -3.3 3,120,440 - RR42_RS14495 0.76 -2.0 3,120,447 - RR42_RS14495 0.76 -2.3 3,120,482 - RR42_RS14495 0.79 -2.4 3,120,502 + RR42_RS14495 0.81 -1.8 3,120,731 - -1.8 3,120,731 - +0.0 3,120,960 + +3.3 3,120,960 + +1.0 3,121,058 - RR42_RS14500 0.22 +0.0 3,121,058 - RR42_RS14500 0.22 +1.0 3,121,058 - RR42_RS14500 0.22 -0.1 3,121,059 + RR42_RS14500 0.23 +1.3 3,121,059 + RR42_RS14500 0.23 -1.2 3,121,060 - RR42_RS14500 0.23 -0.1 3,121,060 - RR42_RS14500 0.23 +0.4 3,121,090 - RR42_RS14500 0.27 +0.8 3,121,176 + RR42_RS14500 0.40 +0.6 3,121,185 + RR42_RS14500 0.41 -1.0 3,121,185 + RR42_RS14500 0.41 +2.0 3,121,186 - RR42_RS14500 0.41 -1.1 3,121,591 + +3.6 3,121,676 + -1.5 3,121,677 - +0.2 3,121,677 - -2.3 3,121,682 + -2.2 3,121,682 + -0.4 3,121,683 - +0.3 3,121,683 - +0.4 3,121,683 - +0.8
Or see this region's nucleotide sequence