Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13970 and RR42_RS13975 are separated by 398 nucleotides RR42_RS13975 and RR42_RS13980 are separated by 437 nucleotides RR42_RS13980 and RR42_RS13985 are separated by 104 nucleotides RR42_RS13985 and RR42_RS13990 are separated by 79 nucleotides
RR42_RS13970: RR42_RS13970 - hypothetical protein, at 3,009,036 to 3,010,262
_RS13970
RR42_RS13975: RR42_RS13975 - chemotaxis protein, at 3,010,661 to 3,012,205
_RS13975
RR42_RS13980: RR42_RS13980 - tRNA-Cys, at 3,012,643 to 3,012,716
_RS13980
RR42_RS13985: RR42_RS13985 - tRNA-Gly, at 3,012,821 to 3,012,896
_RS13985
RR42_RS13990: RR42_RS13990 - tRNA-Gly, at 3,012,976 to 3,013,051
_RS13990
Position (kb)
3010
3011
3012
3013 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3009.662 kb on + strand, within RR42_RS13970 at 3009.870 kb on + strand, within RR42_RS13970 at 3009.982 kb on - strand, within RR42_RS13970 at 3009.982 kb on - strand, within RR42_RS13970 at 3010.114 kb on + strand, within RR42_RS13970 at 3010.125 kb on - strand, within RR42_RS13970 at 3010.156 kb on + strand at 3010.156 kb on + strand at 3010.156 kb on + strand at 3010.156 kb on + strand at 3010.157 kb on - strand at 3010.157 kb on - strand at 3010.203 kb on + strand at 3010.204 kb on - strand at 3010.205 kb on + strand at 3010.206 kb on - strand at 3010.206 kb on - strand at 3010.206 kb on - strand at 3010.250 kb on + strand at 3010.399 kb on + strand at 3010.399 kb on + strand at 3010.435 kb on + strand at 3010.435 kb on + strand at 3010.436 kb on - strand at 3010.507 kb on + strand at 3010.508 kb on - strand at 3010.508 kb on - strand at 3010.508 kb on - strand at 3010.546 kb on + strand at 3010.547 kb on - strand at 3010.647 kb on - strand at 3011.095 kb on + strand, within RR42_RS13975 at 3011.131 kb on + strand, within RR42_RS13975 at 3011.132 kb on - strand, within RR42_RS13975 at 3011.435 kb on + strand, within RR42_RS13975 at 3011.435 kb on + strand, within RR42_RS13975 at 3011.455 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.535 kb on + strand, within RR42_RS13975 at 3011.536 kb on - strand, within RR42_RS13975 at 3011.536 kb on - strand, within RR42_RS13975 at 3011.536 kb on - strand, within RR42_RS13975 at 3011.536 kb on - strand, within RR42_RS13975 at 3011.536 kb on - strand, within RR42_RS13975 at 3011.629 kb on + strand, within RR42_RS13975 at 3011.629 kb on + strand, within RR42_RS13975 at 3011.655 kb on - strand, within RR42_RS13975 at 3011.662 kb on - strand, within RR42_RS13975 at 3011.662 kb on - strand, within RR42_RS13975 at 3011.772 kb on - strand, within RR42_RS13975 at 3011.772 kb on - strand, within RR42_RS13975 at 3011.917 kb on - strand, within RR42_RS13975 at 3011.954 kb on - strand, within RR42_RS13975 at 3012.006 kb on + strand, within RR42_RS13975 at 3012.007 kb on - strand, within RR42_RS13975 at 3012.007 kb on - strand, within RR42_RS13975 at 3012.012 kb on + strand, within RR42_RS13975 at 3012.012 kb on + strand, within RR42_RS13975 at 3012.012 kb on + strand, within RR42_RS13975 at 3012.013 kb on - strand, within RR42_RS13975 at 3012.031 kb on + strand, within RR42_RS13975 at 3012.031 kb on + strand, within RR42_RS13975 at 3012.031 kb on + strand, within RR42_RS13975 at 3012.093 kb on + strand at 3012.094 kb on - strand at 3012.098 kb on - strand at 3012.131 kb on - strand at 3012.183 kb on - strand at 3012.183 kb on - strand at 3012.249 kb on + strand at 3012.250 kb on - strand at 3012.250 kb on - strand at 3012.616 kb on - strand at 3012.641 kb on - strand at 3012.641 kb on - strand at 3012.641 kb on - strand at 3013.097 kb on - strand at 3013.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,009,662 + RR42_RS13970 0.51 +0.7 3,009,870 + RR42_RS13970 0.68 -1.4 3,009,982 - RR42_RS13970 0.77 -0.4 3,009,982 - RR42_RS13970 0.77 +0.6 3,010,114 + RR42_RS13970 0.88 +1.5 3,010,125 - RR42_RS13970 0.89 -0.2 3,010,156 + -0.3 3,010,156 + +1.1 3,010,156 + +0.8 3,010,156 + +0.3 3,010,157 - +0.1 3,010,157 - +0.3 3,010,203 + -0.6 3,010,204 - -2.5 3,010,205 + -1.2 3,010,206 - -2.6 3,010,206 - -1.0 3,010,206 - +0.1 3,010,250 + -0.7 3,010,399 + -0.5 3,010,399 + +0.8 3,010,435 + -0.0 3,010,435 + +1.3 3,010,436 - +0.2 3,010,507 + -0.1 3,010,508 - +1.1 3,010,508 - +3.5 3,010,508 - -0.3 3,010,546 + +1.0 3,010,547 - -2.0 3,010,647 - +0.5 3,011,095 + RR42_RS13975 0.28 -0.7 3,011,131 + RR42_RS13975 0.30 -0.0 3,011,132 - RR42_RS13975 0.30 -0.1 3,011,435 + RR42_RS13975 0.50 -0.9 3,011,435 + RR42_RS13975 0.50 +0.5 3,011,455 + RR42_RS13975 0.51 -1.6 3,011,535 + RR42_RS13975 0.57 -0.2 3,011,535 + RR42_RS13975 0.57 +0.5 3,011,535 + RR42_RS13975 0.57 -0.8 3,011,535 + RR42_RS13975 0.57 +0.1 3,011,535 + RR42_RS13975 0.57 -0.6 3,011,535 + RR42_RS13975 0.57 -1.9 3,011,536 - RR42_RS13975 0.57 -2.0 3,011,536 - RR42_RS13975 0.57 -1.0 3,011,536 - RR42_RS13975 0.57 +0.6 3,011,536 - RR42_RS13975 0.57 -1.9 3,011,536 - RR42_RS13975 0.57 +0.3 3,011,629 + RR42_RS13975 0.63 -0.1 3,011,629 + RR42_RS13975 0.63 -2.2 3,011,655 - RR42_RS13975 0.64 -0.4 3,011,662 - RR42_RS13975 0.65 -1.8 3,011,662 - RR42_RS13975 0.65 -0.0 3,011,772 - RR42_RS13975 0.72 +0.7 3,011,772 - RR42_RS13975 0.72 +1.6 3,011,917 - RR42_RS13975 0.81 -0.2 3,011,954 - RR42_RS13975 0.84 +0.0 3,012,006 + RR42_RS13975 0.87 +0.3 3,012,007 - RR42_RS13975 0.87 -0.9 3,012,007 - RR42_RS13975 0.87 +0.2 3,012,012 + RR42_RS13975 0.87 +0.5 3,012,012 + RR42_RS13975 0.87 -2.0 3,012,012 + RR42_RS13975 0.87 +0.8 3,012,013 - RR42_RS13975 0.88 -0.8 3,012,031 + RR42_RS13975 0.89 -0.3 3,012,031 + RR42_RS13975 0.89 -0.3 3,012,031 + RR42_RS13975 0.89 -2.0 3,012,093 + -0.4 3,012,094 - +0.3 3,012,098 - -0.2 3,012,131 - -1.0 3,012,183 - -0.0 3,012,183 - -1.9 3,012,249 + -0.8 3,012,250 - +0.3 3,012,250 - -0.5 3,012,616 - -0.2 3,012,641 - -2.3 3,012,641 - -1.5 3,012,641 - -0.6 3,013,097 - -0.9 3,013,203 - -1.0
Or see this region's nucleotide sequence