Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13925 and RR42_RS13930 are separated by 29 nucleotides RR42_RS13930 and RR42_RS13935 are separated by 216 nucleotides RR42_RS13935 and RR42_RS13940 are separated by 246 nucleotides RR42_RS13940 and RR42_RS13945 are separated by 197 nucleotides
RR42_RS13925: RR42_RS13925 - biopolymer transporter, at 3,003,083 to 3,003,820
_RS13925
RR42_RS13930: RR42_RS13930 - biopolymer transporter ExbD, at 3,003,850 to 3,004,263
_RS13930
RR42_RS13935: RR42_RS13935 - hemin transporter, at 3,004,480 to 3,004,812
_RS13935
RR42_RS13940: RR42_RS13940 - hypothetical protein, at 3,005,059 to 3,005,466
_RS13940
RR42_RS13945: RR42_RS13945 - hypothetical protein, at 3,005,664 to 3,005,921
_RS13945
Position (kb)
3004
3005 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3003.818 kb on + strand at 3004.262 kb on - strand at 3004.281 kb on + strand at 3004.281 kb on + strand at 3004.282 kb on - strand at 3004.282 kb on - strand at 3004.282 kb on - strand at 3004.364 kb on - strand at 3004.631 kb on + strand, within RR42_RS13935 at 3004.631 kb on + strand, within RR42_RS13935 at 3004.631 kb on + strand, within RR42_RS13935 at 3004.632 kb on - strand, within RR42_RS13935 at 3004.632 kb on - strand, within RR42_RS13935 at 3004.786 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.878 kb on + strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.879 kb on - strand at 3004.912 kb on - strand at 3004.989 kb on - strand at 3005.058 kb on - strand at 3005.058 kb on - strand at 3005.058 kb on - strand at 3005.058 kb on - strand at 3005.080 kb on - strand at 3005.144 kb on - strand, within RR42_RS13940 at 3005.144 kb on - strand, within RR42_RS13940 at 3005.144 kb on - strand, within RR42_RS13940 at 3005.277 kb on + strand, within RR42_RS13940 at 3005.277 kb on + strand, within RR42_RS13940 at 3005.277 kb on + strand, within RR42_RS13940 at 3005.277 kb on + strand, within RR42_RS13940 at 3005.278 kb on - strand, within RR42_RS13940 at 3005.278 kb on - strand, within RR42_RS13940 at 3005.300 kb on + strand, within RR42_RS13940 at 3005.300 kb on + strand, within RR42_RS13940 at 3005.301 kb on - strand, within RR42_RS13940 at 3005.466 kb on + strand at 3005.466 kb on + strand at 3005.466 kb on + strand at 3005.466 kb on + strand at 3005.467 kb on - strand at 3005.467 kb on - strand at 3005.505 kb on - strand at 3005.505 kb on - strand at 3005.505 kb on - strand at 3005.505 kb on - strand at 3005.749 kb on + strand, within RR42_RS13945 at 3005.750 kb on - strand, within RR42_RS13945 at 3005.786 kb on - strand, within RR42_RS13945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,003,818 + -0.4 3,004,262 - -0.2 3,004,281 + +1.0 3,004,281 + -0.3 3,004,282 - -0.7 3,004,282 - +0.2 3,004,282 - -0.1 3,004,364 - -0.0 3,004,631 + RR42_RS13935 0.45 -2.4 3,004,631 + RR42_RS13935 0.45 -2.5 3,004,631 + RR42_RS13935 0.45 -0.8 3,004,632 - RR42_RS13935 0.46 -2.4 3,004,632 - RR42_RS13935 0.46 -1.4 3,004,786 + -2.3 3,004,878 + -0.2 3,004,878 + +0.1 3,004,878 + +0.4 3,004,878 + +0.0 3,004,878 + +0.6 3,004,878 + -1.7 3,004,878 + -1.6 3,004,878 + +0.4 3,004,878 + +0.2 3,004,878 + -0.4 3,004,878 + -2.0 3,004,878 + +0.4 3,004,878 + +0.2 3,004,878 + +0.2 3,004,878 + +0.8 3,004,879 - -0.9 3,004,879 - +0.7 3,004,879 - -0.3 3,004,879 - -1.7 3,004,879 - +0.3 3,004,879 - -0.8 3,004,879 - -0.2 3,004,879 - +0.4 3,004,912 - +0.2 3,004,989 - +1.4 3,005,058 - -0.5 3,005,058 - -3.7 3,005,058 - +0.4 3,005,058 - +1.0 3,005,080 - -1.0 3,005,144 - RR42_RS13940 0.21 -0.0 3,005,144 - RR42_RS13940 0.21 +0.8 3,005,144 - RR42_RS13940 0.21 -0.2 3,005,277 + RR42_RS13940 0.53 +0.3 3,005,277 + RR42_RS13940 0.53 -1.2 3,005,277 + RR42_RS13940 0.53 -2.5 3,005,277 + RR42_RS13940 0.53 -0.6 3,005,278 - RR42_RS13940 0.54 -0.3 3,005,278 - RR42_RS13940 0.54 -0.2 3,005,300 + RR42_RS13940 0.59 -0.5 3,005,300 + RR42_RS13940 0.59 -0.6 3,005,301 - RR42_RS13940 0.59 +0.5 3,005,466 + -0.1 3,005,466 + -0.8 3,005,466 + -1.9 3,005,466 + +0.6 3,005,467 - -0.2 3,005,467 - -0.8 3,005,505 - -0.5 3,005,505 - -0.2 3,005,505 - -0.4 3,005,505 - -1.1 3,005,749 + RR42_RS13945 0.33 +0.3 3,005,750 - RR42_RS13945 0.33 -0.2 3,005,786 - RR42_RS13945 0.47 -2.6
Or see this region's nucleotide sequence