Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12515 and RR42_RS12520 are separated by 56 nucleotides RR42_RS12520 and RR42_RS12525 are separated by 1 nucleotides RR42_RS12525 and RR42_RS12530 are separated by 14 nucleotides RR42_RS12530 and RR42_RS12535 are separated by 207 nucleotides
RR42_RS12515: RR42_RS12515 - camphor resistance protein CrcB, at 2,691,125 to 2,691,505
_RS12515
RR42_RS12520: RR42_RS12520 - molybdenum cofactor biosynthesis protein MoaE, at 2,691,562 to 2,692,032
_RS12520
RR42_RS12525: RR42_RS12525 - molybdenum cofactor biosynthesis protein MoaD, at 2,692,034 to 2,692,291
_RS12525
RR42_RS12530: RR42_RS12530 - molybdenum cofactor biosynthesis protein MoaA, at 2,692,306 to 2,693,547
_RS12530
RR42_RS12535: RR42_RS12535 - threonine synthase, at 2,693,755 to 2,695,203
_RS12535
Position (kb)
2692
2693
2694 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2691.689 kb on + strand, within RR42_RS12520 at 2691.690 kb on - strand, within RR42_RS12520 at 2691.690 kb on - strand, within RR42_RS12520 at 2691.875 kb on + strand, within RR42_RS12520 at 2691.875 kb on + strand, within RR42_RS12520 at 2691.979 kb on + strand, within RR42_RS12520 at 2691.979 kb on + strand, within RR42_RS12520 at 2691.980 kb on - strand, within RR42_RS12520 at 2692.019 kb on - strand at 2692.091 kb on - strand, within RR42_RS12525 at 2692.307 kb on + strand at 2692.308 kb on - strand at 2692.344 kb on + strand at 2692.344 kb on + strand at 2692.344 kb on + strand at 2692.599 kb on + strand, within RR42_RS12530 at 2692.600 kb on - strand, within RR42_RS12530 at 2692.600 kb on - strand, within RR42_RS12530 at 2692.752 kb on + strand, within RR42_RS12530 at 2692.752 kb on + strand, within RR42_RS12530 at 2692.752 kb on + strand, within RR42_RS12530 at 2692.753 kb on - strand, within RR42_RS12530 at 2692.753 kb on - strand, within RR42_RS12530 at 2692.753 kb on - strand, within RR42_RS12530 at 2692.753 kb on - strand, within RR42_RS12530 at 2692.928 kb on + strand, within RR42_RS12530 at 2692.928 kb on + strand, within RR42_RS12530 at 2692.929 kb on - strand, within RR42_RS12530 at 2693.574 kb on + strand at 2693.583 kb on + strand at 2693.583 kb on + strand at 2693.583 kb on + strand at 2693.584 kb on - strand at 2693.584 kb on - strand at 2693.584 kb on - strand at 2693.584 kb on - strand at 2693.584 kb on - strand at 2693.706 kb on + strand at 2693.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,691,689 + RR42_RS12520 0.27 +1.4 2,691,690 - RR42_RS12520 0.27 +1.1 2,691,690 - RR42_RS12520 0.27 +2.1 2,691,875 + RR42_RS12520 0.66 +0.9 2,691,875 + RR42_RS12520 0.66 +1.4 2,691,979 + RR42_RS12520 0.89 +0.9 2,691,979 + RR42_RS12520 0.89 -0.2 2,691,980 - RR42_RS12520 0.89 +1.5 2,692,019 - +0.9 2,692,091 - RR42_RS12525 0.22 +0.7 2,692,307 + +0.9 2,692,308 - -0.4 2,692,344 + +0.3 2,692,344 + +1.0 2,692,344 + +0.3 2,692,599 + RR42_RS12530 0.24 +0.9 2,692,600 - RR42_RS12530 0.24 +1.2 2,692,600 - RR42_RS12530 0.24 +1.9 2,692,752 + RR42_RS12530 0.36 +0.3 2,692,752 + RR42_RS12530 0.36 +1.1 2,692,752 + RR42_RS12530 0.36 +2.2 2,692,753 - RR42_RS12530 0.36 +1.3 2,692,753 - RR42_RS12530 0.36 -0.6 2,692,753 - RR42_RS12530 0.36 +0.7 2,692,753 - RR42_RS12530 0.36 +1.5 2,692,928 + RR42_RS12530 0.50 +0.8 2,692,928 + RR42_RS12530 0.50 +0.6 2,692,929 - RR42_RS12530 0.50 -0.4 2,693,574 + +0.7 2,693,583 + +1.7 2,693,583 + +0.9 2,693,583 + -1.9 2,693,584 - +0.4 2,693,584 - +0.7 2,693,584 - +0.9 2,693,584 - +0.6 2,693,584 - -0.2 2,693,706 + -0.1 2,693,706 + -0.1
Or see this region's nucleotide sequence