Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11750 and RR42_RS11755 are separated by 123 nucleotides RR42_RS11755 and RR42_RS11760 are separated by 118 nucleotides RR42_RS11760 and RR42_RS11765 are separated by 29 nucleotides
RR42_RS11750: RR42_RS11750 - inosine-5-monophosphate dehydrogenase, at 2,513,373 to 2,514,836
_RS11750
RR42_RS11755: RR42_RS11755 - multidrug DMT transporter permease, at 2,514,960 to 2,515,928
_RS11755
RR42_RS11760: RR42_RS11760 - hypothetical protein, at 2,516,047 to 2,516,628
_RS11760
RR42_RS11765: RR42_RS11765 - protein RnfH, at 2,516,658 to 2,517,005
_RS11765
Position (kb)
2514
2515
2516 Strain fitness (log2 ratio)
-2
-1
0
1 at 2514.868 kb on - strand at 2514.921 kb on + strand at 2514.948 kb on - strand at 2514.948 kb on - strand at 2514.966 kb on - strand at 2514.973 kb on + strand at 2514.974 kb on - strand at 2515.030 kb on + strand at 2515.030 kb on + strand at 2515.030 kb on + strand at 2515.031 kb on - strand at 2515.031 kb on - strand at 2515.031 kb on - strand at 2515.031 kb on - strand at 2515.031 kb on - strand at 2515.031 kb on - strand at 2515.194 kb on + strand, within RR42_RS11755 at 2515.195 kb on - strand, within RR42_RS11755 at 2515.197 kb on + strand, within RR42_RS11755 at 2515.197 kb on + strand, within RR42_RS11755 at 2515.197 kb on + strand, within RR42_RS11755 at 2515.197 kb on + strand, within RR42_RS11755 at 2515.197 kb on + strand, within RR42_RS11755 at 2515.198 kb on - strand, within RR42_RS11755 at 2515.198 kb on - strand, within RR42_RS11755 at 2515.198 kb on - strand, within RR42_RS11755 at 2515.198 kb on - strand, within RR42_RS11755 at 2515.198 kb on - strand, within RR42_RS11755 at 2515.370 kb on + strand, within RR42_RS11755 at 2515.370 kb on + strand, within RR42_RS11755 at 2515.370 kb on + strand, within RR42_RS11755 at 2515.371 kb on - strand, within RR42_RS11755 at 2515.494 kb on + strand, within RR42_RS11755 at 2515.495 kb on - strand, within RR42_RS11755 at 2515.515 kb on + strand, within RR42_RS11755 at 2515.515 kb on + strand, within RR42_RS11755 at 2515.515 kb on + strand, within RR42_RS11755 at 2515.563 kb on + strand, within RR42_RS11755 at 2515.563 kb on + strand, within RR42_RS11755 at 2515.563 kb on + strand, within RR42_RS11755 at 2515.563 kb on + strand, within RR42_RS11755 at 2515.740 kb on - strand, within RR42_RS11755 at 2515.786 kb on + strand, within RR42_RS11755 at 2515.786 kb on + strand, within RR42_RS11755 at 2515.787 kb on - strand, within RR42_RS11755 at 2515.927 kb on - strand at 2516.041 kb on + strand at 2516.042 kb on - strand at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.494 kb on + strand, within RR42_RS11760 at 2516.494 kb on + strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.761 kb on + strand, within RR42_RS11765
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,514,868 - -0.1 2,514,921 + -0.4 2,514,948 - -0.4 2,514,948 - -2.0 2,514,966 - -1.5 2,514,973 + +1.5 2,514,974 - +0.3 2,515,030 + -1.2 2,515,030 + -0.8 2,515,030 + -0.3 2,515,031 - -0.8 2,515,031 - -0.9 2,515,031 - -2.4 2,515,031 - -1.6 2,515,031 - -1.5 2,515,031 - -0.2 2,515,194 + RR42_RS11755 0.24 -1.1 2,515,195 - RR42_RS11755 0.24 -2.4 2,515,197 + RR42_RS11755 0.24 -1.3 2,515,197 + RR42_RS11755 0.24 -0.6 2,515,197 + RR42_RS11755 0.24 +0.8 2,515,197 + RR42_RS11755 0.24 +0.2 2,515,197 + RR42_RS11755 0.24 -1.2 2,515,198 - RR42_RS11755 0.25 -1.4 2,515,198 - RR42_RS11755 0.25 -0.3 2,515,198 - RR42_RS11755 0.25 -0.1 2,515,198 - RR42_RS11755 0.25 -0.6 2,515,198 - RR42_RS11755 0.25 +0.3 2,515,370 + RR42_RS11755 0.42 -2.0 2,515,370 + RR42_RS11755 0.42 -0.9 2,515,370 + RR42_RS11755 0.42 -1.4 2,515,371 - RR42_RS11755 0.42 +0.6 2,515,494 + RR42_RS11755 0.55 -0.8 2,515,495 - RR42_RS11755 0.55 -0.8 2,515,515 + RR42_RS11755 0.57 -0.0 2,515,515 + RR42_RS11755 0.57 -0.2 2,515,515 + RR42_RS11755 0.57 +0.7 2,515,563 + RR42_RS11755 0.62 +0.3 2,515,563 + RR42_RS11755 0.62 -0.3 2,515,563 + RR42_RS11755 0.62 -0.9 2,515,563 + RR42_RS11755 0.62 -0.1 2,515,740 - RR42_RS11755 0.80 +0.2 2,515,786 + RR42_RS11755 0.85 +0.6 2,515,786 + RR42_RS11755 0.85 +1.6 2,515,787 - RR42_RS11755 0.85 -1.2 2,515,927 - -0.4 2,516,041 + -1.6 2,516,042 - +0.2 2,516,171 - RR42_RS11760 0.21 +0.5 2,516,171 - RR42_RS11760 0.21 -0.6 2,516,171 - RR42_RS11760 0.21 -0.2 2,516,171 - RR42_RS11760 0.21 -0.4 2,516,494 + RR42_RS11760 0.77 -0.2 2,516,494 + RR42_RS11760 0.77 +0.3 2,516,495 - RR42_RS11760 0.77 -0.6 2,516,495 - RR42_RS11760 0.77 -0.1 2,516,495 - RR42_RS11760 0.77 -0.6 2,516,761 + RR42_RS11765 0.30 +0.6
Or see this region's nucleotide sequence