Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11535 and RR42_RS11540 are separated by 222 nucleotides RR42_RS11540 and RR42_RS11545 are separated by 194 nucleotides RR42_RS11545 and RR42_RS11550 are separated by 89 nucleotides
RR42_RS11535: RR42_RS11535 - hypothetical protein, at 2,465,946 to 2,467,307
_RS11535
RR42_RS11540: RR42_RS11540 - TonB-dependent receptor, at 2,467,530 to 2,469,917
_RS11540
RR42_RS11545: RR42_RS11545 - 4-oxalocrotonate tautomerase, at 2,470,112 to 2,470,348
_RS11545
RR42_RS11550: RR42_RS11550 - 3-oxoacyl-ACP reductase, at 2,470,438 to 2,471,172
_RS11550
Position (kb)
2467
2468
2469
2470 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2466.715 kb on + strand, within RR42_RS11535 at 2466.715 kb on + strand, within RR42_RS11535 at 2466.715 kb on + strand, within RR42_RS11535 at 2466.716 kb on - strand, within RR42_RS11535 at 2466.716 kb on - strand, within RR42_RS11535 at 2466.716 kb on - strand, within RR42_RS11535 at 2466.730 kb on + strand, within RR42_RS11535 at 2466.818 kb on - strand, within RR42_RS11535 at 2466.818 kb on - strand, within RR42_RS11535 at 2466.904 kb on + strand, within RR42_RS11535 at 2467.004 kb on + strand, within RR42_RS11535 at 2467.311 kb on + strand at 2467.312 kb on - strand at 2467.312 kb on - strand at 2467.344 kb on - strand at 2467.387 kb on - strand at 2467.673 kb on - strand at 2467.722 kb on + strand at 2467.723 kb on - strand at 2467.723 kb on - strand at 2467.857 kb on + strand, within RR42_RS11540 at 2467.907 kb on + strand, within RR42_RS11540 at 2467.908 kb on - strand, within RR42_RS11540 at 2467.912 kb on - strand, within RR42_RS11540 at 2468.106 kb on + strand, within RR42_RS11540 at 2468.106 kb on + strand, within RR42_RS11540 at 2468.106 kb on + strand, within RR42_RS11540 at 2468.400 kb on + strand, within RR42_RS11540 at 2468.401 kb on - strand, within RR42_RS11540 at 2468.401 kb on - strand, within RR42_RS11540 at 2468.402 kb on + strand, within RR42_RS11540 at 2468.402 kb on + strand, within RR42_RS11540 at 2468.402 kb on + strand, within RR42_RS11540 at 2468.402 kb on + strand, within RR42_RS11540 at 2468.403 kb on - strand, within RR42_RS11540 at 2468.403 kb on - strand, within RR42_RS11540 at 2468.407 kb on - strand, within RR42_RS11540 at 2468.497 kb on - strand, within RR42_RS11540 at 2468.497 kb on - strand, within RR42_RS11540 at 2468.547 kb on + strand, within RR42_RS11540 at 2468.548 kb on - strand, within RR42_RS11540 at 2468.619 kb on + strand, within RR42_RS11540 at 2468.781 kb on + strand, within RR42_RS11540 at 2468.782 kb on - strand, within RR42_RS11540 at 2468.782 kb on - strand, within RR42_RS11540 at 2468.782 kb on - strand, within RR42_RS11540 at 2468.782 kb on - strand, within RR42_RS11540 at 2468.802 kb on - strand, within RR42_RS11540 at 2468.889 kb on + strand, within RR42_RS11540 at 2468.890 kb on - strand, within RR42_RS11540 at 2468.969 kb on + strand, within RR42_RS11540 at 2468.969 kb on + strand, within RR42_RS11540 at 2468.970 kb on - strand, within RR42_RS11540 at 2468.970 kb on - strand, within RR42_RS11540 at 2468.970 kb on - strand, within RR42_RS11540 at 2469.088 kb on - strand, within RR42_RS11540 at 2469.183 kb on + strand, within RR42_RS11540 at 2469.210 kb on + strand, within RR42_RS11540 at 2469.210 kb on + strand, within RR42_RS11540 at 2469.210 kb on + strand, within RR42_RS11540 at 2469.211 kb on - strand, within RR42_RS11540 at 2469.211 kb on - strand, within RR42_RS11540 at 2469.268 kb on - strand, within RR42_RS11540 at 2469.268 kb on - strand, within RR42_RS11540 at 2469.328 kb on - strand, within RR42_RS11540 at 2469.414 kb on + strand, within RR42_RS11540 at 2469.415 kb on - strand, within RR42_RS11540 at 2469.415 kb on - strand, within RR42_RS11540 at 2469.420 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.435 kb on + strand, within RR42_RS11540 at 2469.658 kb on - strand, within RR42_RS11540 at 2469.735 kb on + strand at 2469.736 kb on - strand at 2469.777 kb on + strand at 2469.778 kb on - strand at 2469.778 kb on - strand at 2469.791 kb on + strand at 2469.791 kb on + strand at 2469.916 kb on - strand at 2469.988 kb on + strand at 2469.988 kb on + strand at 2469.988 kb on + strand at 2469.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,466,715 + RR42_RS11535 0.56 +0.9 2,466,715 + RR42_RS11535 0.56 +0.1 2,466,715 + RR42_RS11535 0.56 +0.5 2,466,716 - RR42_RS11535 0.57 +0.0 2,466,716 - RR42_RS11535 0.57 -0.8 2,466,716 - RR42_RS11535 0.57 -0.1 2,466,730 + RR42_RS11535 0.58 -0.4 2,466,818 - RR42_RS11535 0.64 -0.8 2,466,818 - RR42_RS11535 0.64 -0.1 2,466,904 + RR42_RS11535 0.70 -0.6 2,467,004 + RR42_RS11535 0.78 -0.9 2,467,311 + -1.2 2,467,312 - -0.0 2,467,312 - +0.6 2,467,344 - +0.2 2,467,387 - +0.3 2,467,673 - -0.1 2,467,722 + +1.1 2,467,723 - +0.1 2,467,723 - +0.3 2,467,857 + RR42_RS11540 0.14 +0.3 2,467,907 + RR42_RS11540 0.16 +2.9 2,467,908 - RR42_RS11540 0.16 -2.2 2,467,912 - RR42_RS11540 0.16 -0.0 2,468,106 + RR42_RS11540 0.24 -0.1 2,468,106 + RR42_RS11540 0.24 -0.7 2,468,106 + RR42_RS11540 0.24 +3.4 2,468,400 + RR42_RS11540 0.36 -0.3 2,468,401 - RR42_RS11540 0.36 -0.8 2,468,401 - RR42_RS11540 0.36 +0.6 2,468,402 + RR42_RS11540 0.37 +0.8 2,468,402 + RR42_RS11540 0.37 +0.9 2,468,402 + RR42_RS11540 0.37 -1.1 2,468,402 + RR42_RS11540 0.37 +0.1 2,468,403 - RR42_RS11540 0.37 +1.1 2,468,403 - RR42_RS11540 0.37 +0.4 2,468,407 - RR42_RS11540 0.37 -1.0 2,468,497 - RR42_RS11540 0.40 +0.5 2,468,497 - RR42_RS11540 0.40 +0.9 2,468,547 + RR42_RS11540 0.43 +0.2 2,468,548 - RR42_RS11540 0.43 +0.8 2,468,619 + RR42_RS11540 0.46 -1.1 2,468,781 + RR42_RS11540 0.52 +0.7 2,468,782 - RR42_RS11540 0.52 +0.4 2,468,782 - RR42_RS11540 0.52 +1.3 2,468,782 - RR42_RS11540 0.52 +0.1 2,468,782 - RR42_RS11540 0.52 -2.7 2,468,802 - RR42_RS11540 0.53 -0.3 2,468,889 + RR42_RS11540 0.57 +0.2 2,468,890 - RR42_RS11540 0.57 +0.9 2,468,969 + RR42_RS11540 0.60 -1.1 2,468,969 + RR42_RS11540 0.60 +0.1 2,468,970 - RR42_RS11540 0.60 +0.8 2,468,970 - RR42_RS11540 0.60 +0.3 2,468,970 - RR42_RS11540 0.60 -0.7 2,469,088 - RR42_RS11540 0.65 -0.9 2,469,183 + RR42_RS11540 0.69 +0.5 2,469,210 + RR42_RS11540 0.70 +0.1 2,469,210 + RR42_RS11540 0.70 -1.1 2,469,210 + RR42_RS11540 0.70 +0.1 2,469,211 - RR42_RS11540 0.70 +0.2 2,469,211 - RR42_RS11540 0.70 +0.1 2,469,268 - RR42_RS11540 0.73 -2.7 2,469,268 - RR42_RS11540 0.73 -0.2 2,469,328 - RR42_RS11540 0.75 +0.3 2,469,414 + RR42_RS11540 0.79 +0.8 2,469,415 - RR42_RS11540 0.79 -1.0 2,469,415 - RR42_RS11540 0.79 -0.4 2,469,420 + RR42_RS11540 0.79 +0.4 2,469,435 + RR42_RS11540 0.80 -0.6 2,469,435 + RR42_RS11540 0.80 -0.3 2,469,435 + RR42_RS11540 0.80 -0.9 2,469,435 + RR42_RS11540 0.80 +0.7 2,469,435 + RR42_RS11540 0.80 -0.3 2,469,435 + RR42_RS11540 0.80 +0.2 2,469,658 - RR42_RS11540 0.89 +0.5 2,469,735 + +0.4 2,469,736 - -0.7 2,469,777 + -0.1 2,469,778 - +0.8 2,469,778 - +0.2 2,469,791 + -1.2 2,469,791 + -0.9 2,469,916 - +1.7 2,469,988 + +0.4 2,469,988 + -0.2 2,469,988 + +0.8 2,469,989 - +0.8
Or see this region's nucleotide sequence