Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10345 and RR42_RS10350 are separated by 180 nucleotides RR42_RS10350 and RR42_RS10355 are separated by 171 nucleotides RR42_RS10355 and RR42_RS10360 are separated by 112 nucleotides
RR42_RS10345: RR42_RS10345 - amino acid dehydrogenase, at 2,213,748 to 2,214,818
_RS10345
RR42_RS10350: RR42_RS10350 - indolepyruvate ferredoxin oxidoreductase, at 2,214,999 to 2,218,484
_RS10350
RR42_RS10355: RR42_RS10355 - AsnC family transcriptional regulator, at 2,218,656 to 2,219,126
_RS10355
RR42_RS10360: RR42_RS10360 - porin, at 2,219,239 to 2,220,210
_RS10360
Position (kb)
2214
2215
2216
2217
2218
2219 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2214.011 kb on + strand, within RR42_RS10345 at 2214.123 kb on + strand, within RR42_RS10345 at 2214.282 kb on + strand, within RR42_RS10345 at 2214.283 kb on - strand, within RR42_RS10345 at 2214.466 kb on + strand, within RR42_RS10345 at 2214.467 kb on - strand, within RR42_RS10345 at 2214.467 kb on - strand, within RR42_RS10345 at 2214.619 kb on - strand, within RR42_RS10345 at 2214.797 kb on + strand at 2214.797 kb on + strand at 2214.797 kb on + strand at 2214.798 kb on - strand at 2214.801 kb on + strand at 2214.848 kb on - strand at 2214.942 kb on + strand at 2214.942 kb on + strand at 2214.943 kb on - strand at 2214.943 kb on - strand at 2214.943 kb on - strand at 2214.946 kb on + strand at 2214.946 kb on + strand at 2214.946 kb on + strand at 2214.947 kb on - strand at 2214.950 kb on + strand at 2215.004 kb on - strand at 2215.004 kb on - strand at 2215.125 kb on - strand at 2215.426 kb on + strand, within RR42_RS10350 at 2215.427 kb on - strand, within RR42_RS10350 at 2215.427 kb on - strand, within RR42_RS10350 at 2215.439 kb on - strand, within RR42_RS10350 at 2215.555 kb on + strand, within RR42_RS10350 at 2215.556 kb on - strand, within RR42_RS10350 at 2215.812 kb on - strand, within RR42_RS10350 at 2215.983 kb on + strand, within RR42_RS10350 at 2216.267 kb on + strand, within RR42_RS10350 at 2216.268 kb on - strand, within RR42_RS10350 at 2216.415 kb on + strand, within RR42_RS10350 at 2216.505 kb on - strand, within RR42_RS10350 at 2216.572 kb on - strand, within RR42_RS10350 at 2216.744 kb on - strand, within RR42_RS10350 at 2216.875 kb on - strand, within RR42_RS10350 at 2216.875 kb on - strand, within RR42_RS10350 at 2216.875 kb on - strand, within RR42_RS10350 at 2216.884 kb on + strand, within RR42_RS10350 at 2216.884 kb on + strand, within RR42_RS10350 at 2216.885 kb on - strand, within RR42_RS10350 at 2217.101 kb on - strand, within RR42_RS10350 at 2217.101 kb on - strand, within RR42_RS10350 at 2217.101 kb on - strand, within RR42_RS10350 at 2217.128 kb on + strand, within RR42_RS10350 at 2217.128 kb on + strand, within RR42_RS10350 at 2217.243 kb on - strand, within RR42_RS10350 at 2217.275 kb on - strand, within RR42_RS10350 at 2217.530 kb on - strand, within RR42_RS10350 at 2217.659 kb on + strand, within RR42_RS10350 at 2218.472 kb on - strand at 2218.610 kb on + strand at 2218.611 kb on - strand at 2218.810 kb on - strand, within RR42_RS10355 at 2218.947 kb on + strand, within RR42_RS10355 at 2218.971 kb on + strand, within RR42_RS10355 at 2218.971 kb on + strand, within RR42_RS10355 at 2218.971 kb on + strand, within RR42_RS10355 at 2219.091 kb on + strand at 2219.190 kb on + strand at 2219.250 kb on + strand at 2219.311 kb on - strand at 2219.316 kb on + strand at 2219.319 kb on + strand at 2219.319 kb on + strand at 2219.373 kb on + strand, within RR42_RS10360 at 2219.374 kb on - strand, within RR42_RS10360 at 2219.374 kb on - strand, within RR42_RS10360 at 2219.375 kb on + strand, within RR42_RS10360 at 2219.376 kb on - strand, within RR42_RS10360 at 2219.376 kb on - strand, within RR42_RS10360 at 2219.376 kb on - strand, within RR42_RS10360 at 2219.377 kb on + strand, within RR42_RS10360 at 2219.377 kb on + strand, within RR42_RS10360 at 2219.377 kb on + strand, within RR42_RS10360 at 2219.378 kb on - strand, within RR42_RS10360 at 2219.378 kb on - strand, within RR42_RS10360 at 2219.378 kb on - strand, within RR42_RS10360 at 2219.386 kb on - strand, within RR42_RS10360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,214,011 + RR42_RS10345 0.25 +0.2 2,214,123 + RR42_RS10345 0.35 +0.1 2,214,282 + RR42_RS10345 0.50 -1.6 2,214,283 - RR42_RS10345 0.50 +0.7 2,214,466 + RR42_RS10345 0.67 +0.4 2,214,467 - RR42_RS10345 0.67 +0.2 2,214,467 - RR42_RS10345 0.67 -1.6 2,214,619 - RR42_RS10345 0.81 -0.3 2,214,797 + -0.0 2,214,797 + +1.3 2,214,797 + +0.3 2,214,798 - -0.8 2,214,801 + -1.8 2,214,848 - -0.7 2,214,942 + -0.5 2,214,942 + +0.8 2,214,943 - +0.1 2,214,943 - +0.2 2,214,943 - -0.5 2,214,946 + -1.1 2,214,946 + -1.6 2,214,946 + -2.3 2,214,947 - +0.6 2,214,950 + +0.8 2,215,004 - +1.1 2,215,004 - -1.2 2,215,125 - +0.8 2,215,426 + RR42_RS10350 0.12 +0.7 2,215,427 - RR42_RS10350 0.12 -0.1 2,215,427 - RR42_RS10350 0.12 +1.7 2,215,439 - RR42_RS10350 0.13 -0.2 2,215,555 + RR42_RS10350 0.16 -0.3 2,215,556 - RR42_RS10350 0.16 -0.3 2,215,812 - RR42_RS10350 0.23 +0.8 2,215,983 + RR42_RS10350 0.28 +1.3 2,216,267 + RR42_RS10350 0.36 -0.9 2,216,268 - RR42_RS10350 0.36 +0.7 2,216,415 + RR42_RS10350 0.41 -0.5 2,216,505 - RR42_RS10350 0.43 -0.5 2,216,572 - RR42_RS10350 0.45 -1.1 2,216,744 - RR42_RS10350 0.50 +0.5 2,216,875 - RR42_RS10350 0.54 +0.0 2,216,875 - RR42_RS10350 0.54 -2.3 2,216,875 - RR42_RS10350 0.54 +0.6 2,216,884 + RR42_RS10350 0.54 -0.5 2,216,884 + RR42_RS10350 0.54 -0.8 2,216,885 - RR42_RS10350 0.54 -0.1 2,217,101 - RR42_RS10350 0.60 -0.7 2,217,101 - RR42_RS10350 0.60 -2.1 2,217,101 - RR42_RS10350 0.60 +0.6 2,217,128 + RR42_RS10350 0.61 +0.1 2,217,128 + RR42_RS10350 0.61 -0.6 2,217,243 - RR42_RS10350 0.64 -0.6 2,217,275 - RR42_RS10350 0.65 -1.8 2,217,530 - RR42_RS10350 0.73 +0.7 2,217,659 + RR42_RS10350 0.76 -1.3 2,218,472 - -0.3 2,218,610 + +1.3 2,218,611 - +1.0 2,218,810 - RR42_RS10355 0.33 +1.2 2,218,947 + RR42_RS10355 0.62 -1.1 2,218,971 + RR42_RS10355 0.67 +0.3 2,218,971 + RR42_RS10355 0.67 +0.4 2,218,971 + RR42_RS10355 0.67 +0.3 2,219,091 + -1.2 2,219,190 + +0.5 2,219,250 + +0.6 2,219,311 - -1.6 2,219,316 + -0.7 2,219,319 + +0.8 2,219,319 + -3.1 2,219,373 + RR42_RS10360 0.14 -0.9 2,219,374 - RR42_RS10360 0.14 -0.3 2,219,374 - RR42_RS10360 0.14 -0.5 2,219,375 + RR42_RS10360 0.14 +1.2 2,219,376 - RR42_RS10360 0.14 -0.8 2,219,376 - RR42_RS10360 0.14 +0.8 2,219,376 - RR42_RS10360 0.14 +0.7 2,219,377 + RR42_RS10360 0.14 +0.9 2,219,377 + RR42_RS10360 0.14 +0.1 2,219,377 + RR42_RS10360 0.14 -0.2 2,219,378 - RR42_RS10360 0.14 -0.4 2,219,378 - RR42_RS10360 0.14 +0.4 2,219,378 - RR42_RS10360 0.14 +1.4 2,219,386 - RR42_RS10360 0.15 -0.9
Or see this region's nucleotide sequence