Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10180 and RR42_RS10185 are separated by 82 nucleotides RR42_RS10185 and RR42_RS10190 are separated by 278 nucleotides RR42_RS10190 and RR42_RS10195 are separated by 2 nucleotides
RR42_RS10180: RR42_RS10180 - hypothetical protein, at 2,178,945 to 2,180,213
_RS10180
RR42_RS10185: RR42_RS10185 - hypothetical protein, at 2,180,296 to 2,181,618
_RS10185
RR42_RS10190: RR42_RS10190 - LuxR family transcriptional regulator, at 2,181,897 to 2,182,541
_RS10190
RR42_RS10195: RR42_RS10195 - hypothetical protein, at 2,182,544 to 2,183,359
_RS10195
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2179.622 kb on - strand, within RR42_RS10180 at 2179.654 kb on + strand, within RR42_RS10180 at 2179.655 kb on - strand, within RR42_RS10180 at 2179.758 kb on + strand, within RR42_RS10180 at 2179.900 kb on - strand, within RR42_RS10180 at 2179.900 kb on - strand, within RR42_RS10180 at 2179.975 kb on + strand, within RR42_RS10180 at 2179.975 kb on + strand, within RR42_RS10180 at 2179.976 kb on - strand, within RR42_RS10180 at 2179.993 kb on + strand, within RR42_RS10180 at 2180.024 kb on - strand, within RR42_RS10180 at 2180.046 kb on - strand, within RR42_RS10180 at 2180.046 kb on - strand, within RR42_RS10180 at 2180.063 kb on - strand, within RR42_RS10180 at 2180.063 kb on - strand, within RR42_RS10180 at 2180.182 kb on + strand at 2180.182 kb on + strand at 2180.183 kb on - strand at 2180.317 kb on + strand at 2180.318 kb on - strand at 2180.318 kb on - strand at 2180.411 kb on + strand at 2180.501 kb on + strand, within RR42_RS10185 at 2180.502 kb on - strand, within RR42_RS10185 at 2180.627 kb on + strand, within RR42_RS10185 at 2180.628 kb on - strand, within RR42_RS10185 at 2180.694 kb on + strand, within RR42_RS10185 at 2180.841 kb on - strand, within RR42_RS10185 at 2180.883 kb on + strand, within RR42_RS10185 at 2180.921 kb on + strand, within RR42_RS10185 at 2180.921 kb on + strand, within RR42_RS10185 at 2180.921 kb on + strand, within RR42_RS10185 at 2180.948 kb on + strand, within RR42_RS10185 at 2180.948 kb on + strand, within RR42_RS10185 at 2180.972 kb on - strand, within RR42_RS10185 at 2180.972 kb on - strand, within RR42_RS10185 at 2181.043 kb on + strand, within RR42_RS10185 at 2181.062 kb on + strand, within RR42_RS10185 at 2181.062 kb on + strand, within RR42_RS10185 at 2181.132 kb on + strand, within RR42_RS10185 at 2181.133 kb on - strand, within RR42_RS10185 at 2181.133 kb on - strand, within RR42_RS10185 at 2181.133 kb on - strand, within RR42_RS10185 at 2181.133 kb on - strand, within RR42_RS10185 at 2181.146 kb on + strand, within RR42_RS10185 at 2181.171 kb on - strand, within RR42_RS10185 at 2181.212 kb on + strand, within RR42_RS10185 at 2181.212 kb on + strand, within RR42_RS10185 at 2181.222 kb on - strand, within RR42_RS10185 at 2181.222 kb on - strand, within RR42_RS10185 at 2181.222 kb on - strand, within RR42_RS10185 at 2181.222 kb on - strand, within RR42_RS10185 at 2181.276 kb on - strand, within RR42_RS10185 at 2181.276 kb on - strand, within RR42_RS10185 at 2181.276 kb on - strand, within RR42_RS10185 at 2181.276 kb on - strand, within RR42_RS10185 at 2181.276 kb on - strand, within RR42_RS10185 at 2181.284 kb on + strand, within RR42_RS10185 at 2181.284 kb on + strand, within RR42_RS10185 at 2181.293 kb on + strand, within RR42_RS10185 at 2181.294 kb on - strand, within RR42_RS10185 at 2181.452 kb on + strand, within RR42_RS10185 at 2181.491 kb on + strand at 2181.492 kb on - strand at 2181.492 kb on - strand at 2181.497 kb on + strand at 2181.498 kb on - strand at 2181.508 kb on - strand at 2181.922 kb on + strand at 2181.923 kb on - strand at 2181.983 kb on + strand, within RR42_RS10190 at 2181.983 kb on + strand, within RR42_RS10190 at 2181.984 kb on - strand, within RR42_RS10190 at 2181.987 kb on + strand, within RR42_RS10190 at 2181.988 kb on - strand, within RR42_RS10190 at 2182.149 kb on + strand, within RR42_RS10190 at 2182.165 kb on + strand, within RR42_RS10190 at 2182.166 kb on - strand, within RR42_RS10190 at 2182.166 kb on - strand, within RR42_RS10190 at 2182.166 kb on - strand, within RR42_RS10190 at 2182.166 kb on - strand, within RR42_RS10190 at 2182.213 kb on + strand, within RR42_RS10190 at 2182.235 kb on - strand, within RR42_RS10190 at 2182.359 kb on + strand, within RR42_RS10190 at 2182.556 kb on + strand at 2182.556 kb on + strand at 2182.557 kb on - strand at 2182.557 kb on - strand at 2182.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,179,622 - RR42_RS10180 0.53 -0.6 2,179,654 + RR42_RS10180 0.56 +0.7 2,179,655 - RR42_RS10180 0.56 -0.4 2,179,758 + RR42_RS10180 0.64 +0.1 2,179,900 - RR42_RS10180 0.75 +0.6 2,179,900 - RR42_RS10180 0.75 +1.6 2,179,975 + RR42_RS10180 0.81 -3.1 2,179,975 + RR42_RS10180 0.81 -0.8 2,179,976 - RR42_RS10180 0.81 +3.3 2,179,993 + RR42_RS10180 0.83 +0.3 2,180,024 - RR42_RS10180 0.85 -1.6 2,180,046 - RR42_RS10180 0.87 +0.6 2,180,046 - RR42_RS10180 0.87 -0.1 2,180,063 - RR42_RS10180 0.88 -2.1 2,180,063 - RR42_RS10180 0.88 +0.3 2,180,182 + +0.0 2,180,182 + -1.3 2,180,183 - -1.1 2,180,317 + +0.8 2,180,318 - -1.9 2,180,318 - +1.2 2,180,411 + -0.4 2,180,501 + RR42_RS10185 0.15 +0.3 2,180,502 - RR42_RS10185 0.16 +0.2 2,180,627 + RR42_RS10185 0.25 +0.7 2,180,628 - RR42_RS10185 0.25 -0.2 2,180,694 + RR42_RS10185 0.30 +0.2 2,180,841 - RR42_RS10185 0.41 -0.1 2,180,883 + RR42_RS10185 0.44 -0.9 2,180,921 + RR42_RS10185 0.47 -0.0 2,180,921 + RR42_RS10185 0.47 -0.1 2,180,921 + RR42_RS10185 0.47 -0.9 2,180,948 + RR42_RS10185 0.49 -0.6 2,180,948 + RR42_RS10185 0.49 +1.2 2,180,972 - RR42_RS10185 0.51 +0.7 2,180,972 - RR42_RS10185 0.51 +0.4 2,181,043 + RR42_RS10185 0.56 -0.4 2,181,062 + RR42_RS10185 0.58 -0.6 2,181,062 + RR42_RS10185 0.58 +0.2 2,181,132 + RR42_RS10185 0.63 +0.5 2,181,133 - RR42_RS10185 0.63 +0.8 2,181,133 - RR42_RS10185 0.63 -0.2 2,181,133 - RR42_RS10185 0.63 +0.4 2,181,133 - RR42_RS10185 0.63 +2.0 2,181,146 + RR42_RS10185 0.64 -0.9 2,181,171 - RR42_RS10185 0.66 +1.9 2,181,212 + RR42_RS10185 0.69 +0.3 2,181,212 + RR42_RS10185 0.69 -0.1 2,181,222 - RR42_RS10185 0.70 +0.6 2,181,222 - RR42_RS10185 0.70 -0.7 2,181,222 - RR42_RS10185 0.70 -0.2 2,181,222 - RR42_RS10185 0.70 +0.4 2,181,276 - RR42_RS10185 0.74 +0.4 2,181,276 - RR42_RS10185 0.74 +0.2 2,181,276 - RR42_RS10185 0.74 -0.8 2,181,276 - RR42_RS10185 0.74 -1.0 2,181,276 - RR42_RS10185 0.74 +0.9 2,181,284 + RR42_RS10185 0.75 -1.1 2,181,284 + RR42_RS10185 0.75 +0.6 2,181,293 + RR42_RS10185 0.75 -0.7 2,181,294 - RR42_RS10185 0.75 -0.6 2,181,452 + RR42_RS10185 0.87 -0.3 2,181,491 + +1.2 2,181,492 - +0.7 2,181,492 - -1.5 2,181,497 + -0.0 2,181,498 - +1.0 2,181,508 - -0.7 2,181,922 + -0.9 2,181,923 - +0.9 2,181,983 + RR42_RS10190 0.13 -0.8 2,181,983 + RR42_RS10190 0.13 -0.4 2,181,984 - RR42_RS10190 0.13 -0.0 2,181,987 + RR42_RS10190 0.14 -0.3 2,181,988 - RR42_RS10190 0.14 -0.3 2,182,149 + RR42_RS10190 0.39 +0.1 2,182,165 + RR42_RS10190 0.42 +0.5 2,182,166 - RR42_RS10190 0.42 -0.2 2,182,166 - RR42_RS10190 0.42 -1.9 2,182,166 - RR42_RS10190 0.42 -1.3 2,182,166 - RR42_RS10190 0.42 +0.6 2,182,213 + RR42_RS10190 0.49 -0.5 2,182,235 - RR42_RS10190 0.52 -2.1 2,182,359 + RR42_RS10190 0.72 +0.5 2,182,556 + +0.5 2,182,556 + -0.2 2,182,557 - -1.0 2,182,557 - -0.0 2,182,557 - +0.9
Or see this region's nucleotide sequence