Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10015 and RR42_RS10020 are separated by 58 nucleotides RR42_RS10020 and RR42_RS10025 are separated by 93 nucleotides RR42_RS10025 and RR42_RS10030 are separated by 324 nucleotides
RR42_RS10015: RR42_RS10015 - 4-methylmuconolactone transporter, at 2,139,503 to 2,140,810
_RS10015
RR42_RS10020: RR42_RS10020 - 4-methylmuconolactone methylisomerase, at 2,140,869 to 2,141,207
_RS10020
RR42_RS10025: RR42_RS10025 - muconolactone delta-isomerase, at 2,141,301 to 2,141,576
_RS10025
RR42_RS10030: RR42_RS10030 - 3-hydroxyisobutyrate dehydrogenase, at 2,141,901 to 2,142,290
_RS10030
Position (kb)
2140
2141
2142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2140.158 kb on - strand, within RR42_RS10015 at 2140.186 kb on - strand, within RR42_RS10015 at 2140.186 kb on - strand, within RR42_RS10015 at 2140.214 kb on + strand, within RR42_RS10015 at 2140.215 kb on - strand, within RR42_RS10015 at 2140.287 kb on + strand, within RR42_RS10015 at 2140.287 kb on + strand, within RR42_RS10015 at 2140.288 kb on - strand, within RR42_RS10015 at 2140.409 kb on + strand, within RR42_RS10015 at 2140.409 kb on + strand, within RR42_RS10015 at 2140.410 kb on - strand, within RR42_RS10015 at 2140.537 kb on + strand, within RR42_RS10015 at 2140.537 kb on + strand, within RR42_RS10015 at 2140.538 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.662 kb on - strand, within RR42_RS10015 at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.839 kb on + strand at 2140.840 kb on - strand at 2140.884 kb on + strand at 2140.983 kb on + strand, within RR42_RS10020 at 2140.983 kb on + strand, within RR42_RS10020 at 2141.091 kb on + strand, within RR42_RS10020 at 2141.100 kb on + strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.307 kb on + strand at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.449 kb on - strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.668 kb on + strand at 2141.977 kb on + strand, within RR42_RS10030 at 2141.977 kb on + strand, within RR42_RS10030 at 2141.977 kb on + strand, within RR42_RS10030 at 2141.978 kb on - strand, within RR42_RS10030 at 2141.978 kb on - strand, within RR42_RS10030 at 2142.001 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.059 kb on - strand, within RR42_RS10030 at 2142.193 kb on + strand, within RR42_RS10030 at 2142.193 kb on + strand, within RR42_RS10030 at 2142.194 kb on - strand, within RR42_RS10030 at 2142.194 kb on - strand, within RR42_RS10030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,140,158 - RR42_RS10015 0.50 -0.9 2,140,186 - RR42_RS10015 0.52 -0.5 2,140,186 - RR42_RS10015 0.52 -1.3 2,140,214 + RR42_RS10015 0.54 -0.3 2,140,215 - RR42_RS10015 0.54 +0.3 2,140,287 + RR42_RS10015 0.60 -0.6 2,140,287 + RR42_RS10015 0.60 +0.0 2,140,288 - RR42_RS10015 0.60 -2.5 2,140,409 + RR42_RS10015 0.69 -1.0 2,140,409 + RR42_RS10015 0.69 +0.7 2,140,410 - RR42_RS10015 0.69 +0.9 2,140,537 + RR42_RS10015 0.79 -2.9 2,140,537 + RR42_RS10015 0.79 +0.4 2,140,538 - RR42_RS10015 0.79 -1.1 2,140,662 - RR42_RS10015 0.89 +0.1 2,140,662 - RR42_RS10015 0.89 -0.4 2,140,662 - RR42_RS10015 0.89 +0.7 2,140,839 + -1.6 2,140,839 + -0.4 2,140,839 + -1.2 2,140,839 + +0.9 2,140,839 + -0.5 2,140,839 + +0.4 2,140,840 - +0.8 2,140,884 + +0.9 2,140,983 + RR42_RS10020 0.34 -0.3 2,140,983 + RR42_RS10020 0.34 -2.8 2,141,091 + RR42_RS10020 0.65 -1.6 2,141,100 + RR42_RS10020 0.68 -2.1 2,141,101 - RR42_RS10020 0.68 -0.5 2,141,101 - RR42_RS10020 0.68 +1.3 2,141,101 - RR42_RS10020 0.68 +0.4 2,141,307 + -0.6 2,141,428 + RR42_RS10025 0.46 +0.5 2,141,428 + RR42_RS10025 0.46 +1.9 2,141,428 + RR42_RS10025 0.46 -0.9 2,141,428 + RR42_RS10025 0.46 +4.4 2,141,429 - RR42_RS10025 0.46 +1.8 2,141,429 - RR42_RS10025 0.46 +1.7 2,141,449 - RR42_RS10025 0.54 -0.3 2,141,526 + RR42_RS10025 0.82 -0.3 2,141,526 + RR42_RS10025 0.82 -0.1 2,141,527 - RR42_RS10025 0.82 +2.0 2,141,527 - RR42_RS10025 0.82 -0.4 2,141,528 + RR42_RS10025 0.82 +1.7 2,141,528 + RR42_RS10025 0.82 -0.1 2,141,528 + RR42_RS10025 0.82 -0.6 2,141,528 + RR42_RS10025 0.82 -0.6 2,141,528 + RR42_RS10025 0.82 +0.4 2,141,529 - RR42_RS10025 0.83 +0.1 2,141,529 - RR42_RS10025 0.83 +0.8 2,141,529 - RR42_RS10025 0.83 +1.4 2,141,529 - RR42_RS10025 0.83 +0.5 2,141,529 - RR42_RS10025 0.83 +0.4 2,141,529 - RR42_RS10025 0.83 -0.3 2,141,668 + +1.3 2,141,977 + RR42_RS10030 0.19 +0.5 2,141,977 + RR42_RS10030 0.19 -0.9 2,141,977 + RR42_RS10030 0.19 -0.0 2,141,978 - RR42_RS10030 0.20 -0.6 2,141,978 - RR42_RS10030 0.20 +0.7 2,142,001 + RR42_RS10030 0.26 -1.9 2,142,058 + RR42_RS10030 0.40 -0.1 2,142,058 + RR42_RS10030 0.40 +0.7 2,142,058 + RR42_RS10030 0.40 -0.3 2,142,058 + RR42_RS10030 0.40 -0.1 2,142,058 + RR42_RS10030 0.40 -1.2 2,142,059 - RR42_RS10030 0.41 -0.4 2,142,193 + RR42_RS10030 0.75 -0.8 2,142,193 + RR42_RS10030 0.75 -1.0 2,142,194 - RR42_RS10030 0.75 +0.4 2,142,194 - RR42_RS10030 0.75 +0.4
Or see this region's nucleotide sequence