Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09045 and RR42_RS09050 are separated by 72 nucleotides RR42_RS09050 and RR42_RS09055 overlap by 14 nucleotides RR42_RS09055 and RR42_RS09060 are separated by 12 nucleotides
RR42_RS09045: RR42_RS09045 - peptidylprolyl isomerase, at 1,901,200 to 1,903,149
_RS09045
RR42_RS09050: RR42_RS09050 - arylesterase, at 1,903,222 to 1,903,917
_RS09050
RR42_RS09055: RR42_RS09055 - ABC transporter, at 1,903,904 to 1,904,602
_RS09055
RR42_RS09060: RR42_RS09060 - glucose-6-phosphate isomerase, at 1,904,615 to 1,906,249
_RS09060
Position (kb) 1903
1904 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1902.245 kb on - strand, within RR42_RS09045 at 1902.289 kb on + strand, within RR42_RS09045 at 1902.403 kb on + strand, within RR42_RS09045 at 1902.432 kb on + strand, within RR42_RS09045 at 1902.433 kb on - strand, within RR42_RS09045 at 1902.439 kb on + strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.724 kb on + strand, within RR42_RS09045 at 1902.724 kb on + strand, within RR42_RS09045 at 1902.725 kb on - strand, within RR42_RS09045 at 1902.725 kb on - strand, within RR42_RS09045 at 1902.726 kb on + strand, within RR42_RS09045 at 1902.727 kb on - strand, within RR42_RS09045 at 1902.727 kb on - strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.911 kb on - strand, within RR42_RS09045 at 1902.940 kb on + strand, within RR42_RS09045 at 1902.949 kb on + strand, within RR42_RS09045 at 1902.950 kb on - strand, within RR42_RS09045 at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.223 kb on + strand at 1903.223 kb on + strand at 1903.224 kb on - strand at 1903.224 kb on - strand at 1903.231 kb on - strand at 1903.416 kb on - strand, within RR42_RS09050 at 1903.452 kb on - strand, within RR42_RS09050 at 1903.683 kb on + strand, within RR42_RS09050 at 1903.683 kb on + strand, within RR42_RS09050 at 1903.684 kb on - strand, within RR42_RS09050 at 1903.684 kb on - strand, within RR42_RS09050 at 1904.325 kb on - strand, within RR42_RS09055 at 1904.603 kb on + strand at 1904.704 kb on + strand at 1904.705 kb on - strand at 1904.811 kb on + strand, within RR42_RS09060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,902,245 - RR42_RS09045 0.54 +0.9 1,902,289 + RR42_RS09045 0.56 +0.2 1,902,403 + RR42_RS09045 0.62 +0.6 1,902,432 + RR42_RS09045 0.63 +0.8 1,902,433 - RR42_RS09045 0.63 +1.0 1,902,439 + RR42_RS09045 0.64 -1.5 1,902,440 - RR42_RS09045 0.64 +0.0 1,902,440 - RR42_RS09045 0.64 -1.3 1,902,440 - RR42_RS09045 0.64 +1.4 1,902,724 + RR42_RS09045 0.78 -0.2 1,902,724 + RR42_RS09045 0.78 +1.9 1,902,725 - RR42_RS09045 0.78 -0.0 1,902,725 - RR42_RS09045 0.78 +1.0 1,902,726 + RR42_RS09045 0.78 +2.8 1,902,727 - RR42_RS09045 0.78 +2.8 1,902,727 - RR42_RS09045 0.78 +1.4 1,902,910 + RR42_RS09045 0.88 -0.8 1,902,910 + RR42_RS09045 0.88 -0.0 1,902,910 + RR42_RS09045 0.88 +0.9 1,902,910 + RR42_RS09045 0.88 +0.4 1,902,910 + RR42_RS09045 0.88 +0.6 1,902,911 - RR42_RS09045 0.88 +0.1 1,902,940 + RR42_RS09045 0.89 +1.2 1,902,949 + RR42_RS09045 0.90 +1.3 1,902,950 - RR42_RS09045 0.90 +0.3 1,903,058 - +1.1 1,903,058 - +0.0 1,903,058 - +0.8 1,903,058 - +1.2 1,903,058 - +0.8 1,903,058 - +1.8 1,903,223 + -1.2 1,903,223 + -0.8 1,903,224 - -0.2 1,903,224 - +0.6 1,903,231 - +0.7 1,903,416 - RR42_RS09050 0.28 -0.1 1,903,452 - RR42_RS09050 0.33 -0.1 1,903,683 + RR42_RS09050 0.66 +0.4 1,903,683 + RR42_RS09050 0.66 -0.0 1,903,684 - RR42_RS09050 0.66 -0.8 1,903,684 - RR42_RS09050 0.66 -1.3 1,904,325 - RR42_RS09055 0.60 +0.2 1,904,603 + -0.6 1,904,704 + -2.2 1,904,705 - +0.3 1,904,811 + RR42_RS09060 0.12 +0.5
Or see this region's nucleotide sequence