Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06575 and RR42_RS06580 are separated by 62 nucleotides RR42_RS06580 and RR42_RS06585 are separated by 15 nucleotides RR42_RS06585 and RR42_RS06590 are separated by 21 nucleotides RR42_RS06590 and RR42_RS06595 are separated by 67 nucleotides RR42_RS06595 and RR42_RS06600 are separated by 367 nucleotides
RR42_RS06575: RR42_RS06575 - alpha/beta hydrolase, at 1,436,778 to 1,437,665
_RS06575
RR42_RS06580: RR42_RS06580 - aminoacrylate peracid reductase, at 1,437,728 to 1,438,090
_RS06580
RR42_RS06585: RR42_RS06585 - GTP pyrophosphokinase, at 1,438,106 to 1,440,328
_RS06585
RR42_RS06590: RR42_RS06590 - tRNA-Val, at 1,440,350 to 1,440,426
_RS06590
RR42_RS06595: RR42_RS06595 - tRNA-Val, at 1,440,494 to 1,440,570
_RS06595
RR42_RS06600: RR42_RS06600 - threonyl-tRNA synthetase, at 1,440,938 to 1,442,845
_RS06600
Position (kb)
1438
1439
1440
1441 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.388 kb on - strand, within RR42_RS06575 at 1437.499 kb on + strand, within RR42_RS06575 at 1437.639 kb on - strand at 1437.717 kb on - strand at 1437.780 kb on - strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1438.006 kb on + strand, within RR42_RS06580 at 1438.053 kb on - strand, within RR42_RS06580 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585 at 1438.721 kb on - strand, within RR42_RS06585 at 1438.724 kb on + strand, within RR42_RS06585 at 1438.724 kb on + strand, within RR42_RS06585 at 1438.724 kb on + strand, within RR42_RS06585 at 1438.724 kb on + strand, within RR42_RS06585 at 1438.725 kb on - strand, within RR42_RS06585 at 1438.725 kb on - strand, within RR42_RS06585 at 1438.725 kb on - strand, within RR42_RS06585 at 1438.775 kb on + strand, within RR42_RS06585 at 1438.775 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.858 kb on + strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.859 kb on - strand, within RR42_RS06585 at 1438.863 kb on - strand, within RR42_RS06585 at 1438.863 kb on - strand, within RR42_RS06585 at 1438.863 kb on - strand, within RR42_RS06585 at 1438.863 kb on - strand, within RR42_RS06585 at 1438.913 kb on + strand, within RR42_RS06585 at 1438.913 kb on + strand, within RR42_RS06585 at 1438.914 kb on - strand, within RR42_RS06585 at 1438.962 kb on - strand, within RR42_RS06585 at 1438.987 kb on + strand, within RR42_RS06585 at 1438.988 kb on - strand, within RR42_RS06585 at 1438.988 kb on - strand, within RR42_RS06585 at 1438.988 kb on - strand, within RR42_RS06585 at 1438.988 kb on - strand, within RR42_RS06585 at 1439.021 kb on + strand, within RR42_RS06585 at 1439.021 kb on + strand, within RR42_RS06585 at 1439.022 kb on - strand, within RR42_RS06585 at 1439.022 kb on - strand, within RR42_RS06585 at 1439.162 kb on + strand, within RR42_RS06585 at 1439.189 kb on - strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.339 kb on + strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.340 kb on - strand, within RR42_RS06585 at 1439.405 kb on + strand, within RR42_RS06585 at 1439.408 kb on + strand, within RR42_RS06585 at 1439.408 kb on + strand, within RR42_RS06585 at 1439.408 kb on + strand, within RR42_RS06585 at 1439.409 kb on - strand, within RR42_RS06585 at 1439.417 kb on - strand, within RR42_RS06585 at 1439.417 kb on - strand, within RR42_RS06585 at 1439.417 kb on - strand, within RR42_RS06585 at 1439.565 kb on - strand, within RR42_RS06585 at 1440.070 kb on + strand, within RR42_RS06585 at 1440.349 kb on + strand at 1440.357 kb on + strand at 1440.422 kb on + strand at 1440.439 kb on + strand at 1440.488 kb on + strand at 1440.566 kb on + strand at 1440.576 kb on + strand at 1440.576 kb on + strand at 1440.576 kb on + strand at 1440.582 kb on + strand at 1440.667 kb on + strand at 1440.781 kb on + strand at 1440.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,437,222 + RR42_RS06575 0.50 +1.1 1,437,222 + RR42_RS06575 0.50 +0.3 1,437,223 - RR42_RS06575 0.50 -1.7 1,437,223 - RR42_RS06575 0.50 -3.3 1,437,388 - RR42_RS06575 0.69 -3.0 1,437,499 + RR42_RS06575 0.81 +0.1 1,437,639 - -3.9 1,437,717 - -4.0 1,437,780 - RR42_RS06580 0.14 -1.7 1,437,803 + RR42_RS06580 0.21 -1.4 1,437,803 + RR42_RS06580 0.21 -2.3 1,437,804 - RR42_RS06580 0.21 -3.4 1,437,804 - RR42_RS06580 0.21 -2.8 1,438,006 + RR42_RS06580 0.77 -2.5 1,438,053 - RR42_RS06580 0.90 -1.5 1,438,411 + RR42_RS06585 0.14 -2.4 1,438,411 + RR42_RS06585 0.14 -5.3 1,438,637 + RR42_RS06585 0.24 -2.1 1,438,637 + RR42_RS06585 0.24 -3.1 1,438,721 - RR42_RS06585 0.28 -0.6 1,438,724 + RR42_RS06585 0.28 -3.4 1,438,724 + RR42_RS06585 0.28 -3.2 1,438,724 + RR42_RS06585 0.28 -1.4 1,438,724 + RR42_RS06585 0.28 -2.1 1,438,725 - RR42_RS06585 0.28 -1.7 1,438,725 - RR42_RS06585 0.28 -1.1 1,438,725 - RR42_RS06585 0.28 -3.0 1,438,775 + RR42_RS06585 0.30 -2.8 1,438,775 + RR42_RS06585 0.30 -3.8 1,438,858 + RR42_RS06585 0.34 -3.1 1,438,858 + RR42_RS06585 0.34 -3.9 1,438,858 + RR42_RS06585 0.34 -3.4 1,438,858 + RR42_RS06585 0.34 -1.9 1,438,858 + RR42_RS06585 0.34 -3.0 1,438,858 + RR42_RS06585 0.34 -3.0 1,438,858 + RR42_RS06585 0.34 -2.9 1,438,858 + RR42_RS06585 0.34 -0.4 1,438,858 + RR42_RS06585 0.34 -3.8 1,438,859 - RR42_RS06585 0.34 -1.6 1,438,859 - RR42_RS06585 0.34 -2.8 1,438,859 - RR42_RS06585 0.34 -1.7 1,438,859 - RR42_RS06585 0.34 -1.7 1,438,859 - RR42_RS06585 0.34 -1.2 1,438,859 - RR42_RS06585 0.34 -2.1 1,438,859 - RR42_RS06585 0.34 -1.4 1,438,859 - RR42_RS06585 0.34 -0.1 1,438,859 - RR42_RS06585 0.34 -2.7 1,438,863 - RR42_RS06585 0.34 -3.3 1,438,863 - RR42_RS06585 0.34 -4.5 1,438,863 - RR42_RS06585 0.34 -4.3 1,438,863 - RR42_RS06585 0.34 -3.6 1,438,913 + RR42_RS06585 0.36 -2.6 1,438,913 + RR42_RS06585 0.36 -3.6 1,438,914 - RR42_RS06585 0.36 -1.9 1,438,962 - RR42_RS06585 0.39 -0.1 1,438,987 + RR42_RS06585 0.40 -2.3 1,438,988 - RR42_RS06585 0.40 -3.9 1,438,988 - RR42_RS06585 0.40 -2.6 1,438,988 - RR42_RS06585 0.40 -2.2 1,438,988 - RR42_RS06585 0.40 -2.6 1,439,021 + RR42_RS06585 0.41 -3.4 1,439,021 + RR42_RS06585 0.41 -4.4 1,439,022 - RR42_RS06585 0.41 -3.5 1,439,022 - RR42_RS06585 0.41 -4.4 1,439,162 + RR42_RS06585 0.48 -3.0 1,439,189 - RR42_RS06585 0.49 -2.4 1,439,339 + RR42_RS06585 0.55 -5.5 1,439,339 + RR42_RS06585 0.55 -4.4 1,439,339 + RR42_RS06585 0.55 -1.4 1,439,339 + RR42_RS06585 0.55 -2.1 1,439,339 + RR42_RS06585 0.55 -3.0 1,439,339 + RR42_RS06585 0.55 -1.1 1,439,339 + RR42_RS06585 0.55 -3.1 1,439,340 - RR42_RS06585 0.56 -2.5 1,439,340 - RR42_RS06585 0.56 -2.4 1,439,340 - RR42_RS06585 0.56 -3.2 1,439,340 - RR42_RS06585 0.56 -3.3 1,439,340 - RR42_RS06585 0.56 -1.9 1,439,340 - RR42_RS06585 0.56 -5.2 1,439,340 - RR42_RS06585 0.56 -1.4 1,439,405 + RR42_RS06585 0.58 -1.8 1,439,408 + RR42_RS06585 0.59 -1.6 1,439,408 + RR42_RS06585 0.59 -1.9 1,439,408 + RR42_RS06585 0.59 -3.6 1,439,409 - RR42_RS06585 0.59 -3.6 1,439,417 - RR42_RS06585 0.59 -2.6 1,439,417 - RR42_RS06585 0.59 -3.1 1,439,417 - RR42_RS06585 0.59 -3.6 1,439,565 - RR42_RS06585 0.66 -0.9 1,440,070 + RR42_RS06585 0.88 -3.1 1,440,349 + -2.4 1,440,357 + -0.3 1,440,422 + -1.0 1,440,439 + +0.7 1,440,488 + -2.3 1,440,566 + +0.5 1,440,576 + -0.4 1,440,576 + -0.3 1,440,576 + -0.3 1,440,582 + +0.4 1,440,667 + +0.9 1,440,781 + +0.3 1,440,869 + -0.5
Or see this region's nucleotide sequence