Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06345 and RR42_RS06350 are separated by 254 nucleotides RR42_RS06350 and RR42_RS06355 overlap by 4 nucleotides
RR42_RS06345: RR42_RS06345 - hypothetical protein, at 1,391,303 to 1,392,001
_RS06345
RR42_RS06350: RR42_RS06350 - cysteinyl-tRNA synthetase, at 1,392,256 to 1,393,644
_RS06350
RR42_RS06355: RR42_RS06355 - Fe-S cluster assembly protein HesB, at 1,393,641 to 1,394,567
_RS06355
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1391.300 kb on + strand at 1391.301 kb on - strand at 1391.301 kb on - strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.305 kb on - strand at 1391.454 kb on + strand, within RR42_RS06345 at 1391.454 kb on + strand, within RR42_RS06345 at 1391.500 kb on - strand, within RR42_RS06345 at 1391.581 kb on - strand, within RR42_RS06345 at 1391.581 kb on - strand, within RR42_RS06345 at 1391.767 kb on - strand, within RR42_RS06345 at 1391.979 kb on - strand at 1392.003 kb on + strand at 1392.003 kb on + strand at 1392.004 kb on - strand at 1392.004 kb on - strand at 1392.020 kb on - strand at 1392.035 kb on - strand at 1392.163 kb on + strand at 1392.163 kb on + strand at 1392.163 kb on + strand at 1392.163 kb on + strand at 1394.028 kb on + strand, within RR42_RS06355 at 1394.028 kb on + strand, within RR42_RS06355 at 1394.233 kb on - strand, within RR42_RS06355 at 1394.282 kb on + strand, within RR42_RS06355 at 1394.282 kb on + strand, within RR42_RS06355 at 1394.282 kb on + strand, within RR42_RS06355 at 1394.282 kb on + strand, within RR42_RS06355 at 1394.283 kb on - strand, within RR42_RS06355 at 1394.442 kb on + strand, within RR42_RS06355 at 1394.526 kb on + strand at 1394.527 kb on - strand at 1394.527 kb on - strand at 1394.527 kb on - strand at 1394.562 kb on + strand at 1394.562 kb on + strand at 1394.562 kb on + strand at 1394.562 kb on + strand at 1394.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,391,300 + -0.2 1,391,301 - -1.1 1,391,301 - -0.0 1,391,304 + -1.7 1,391,304 + +0.2 1,391,304 + -0.2 1,391,304 + +0.9 1,391,305 - -0.2 1,391,454 + RR42_RS06345 0.22 -2.8 1,391,454 + RR42_RS06345 0.22 -3.2 1,391,500 - RR42_RS06345 0.28 -0.9 1,391,581 - RR42_RS06345 0.40 -2.1 1,391,581 - RR42_RS06345 0.40 -4.7 1,391,767 - RR42_RS06345 0.66 -3.4 1,391,979 - -2.5 1,392,003 + -3.3 1,392,003 + -3.5 1,392,004 - +0.2 1,392,004 - -0.4 1,392,020 - -0.6 1,392,035 - -0.2 1,392,163 + +0.8 1,392,163 + -0.4 1,392,163 + -0.5 1,392,163 + +1.9 1,394,028 + RR42_RS06355 0.42 -3.5 1,394,028 + RR42_RS06355 0.42 -0.7 1,394,233 - RR42_RS06355 0.64 -1.2 1,394,282 + RR42_RS06355 0.69 -1.4 1,394,282 + RR42_RS06355 0.69 -2.1 1,394,282 + RR42_RS06355 0.69 -0.7 1,394,282 + RR42_RS06355 0.69 -2.1 1,394,283 - RR42_RS06355 0.69 -1.8 1,394,442 + RR42_RS06355 0.86 -1.2 1,394,526 + -1.6 1,394,527 - +0.7 1,394,527 - +0.2 1,394,527 - -0.2 1,394,562 + -0.2 1,394,562 + +0.5 1,394,562 + -0.6 1,394,562 + +1.0 1,394,565 + -0.7
Or see this region's nucleotide sequence