Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06240 and RR42_RS06245 are separated by 14 nucleotides RR42_RS06245 and RR42_RS06250 are separated by 150 nucleotides RR42_RS06250 and RR42_RS06255 are separated by 45 nucleotides
RR42_RS06240: RR42_RS06240 - tryptophan--tRNA ligase, at 1,369,147 to 1,370,349
_RS06240
RR42_RS06245: RR42_RS06245 - SAM-dependent methyltransferase, at 1,370,364 to 1,370,942
_RS06245
RR42_RS06250: RR42_RS06250 - dihydrodipicolinate synthase, at 1,371,093 to 1,371,977
_RS06250
RR42_RS06255: RR42_RS06255 - lipoprotein, at 1,372,023 to 1,373,234
_RS06255
Position (kb) 1371
1372 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1370.646 kb on + strand, within RR42_RS06245 at 1370.647 kb on - strand, within RR42_RS06245 at 1370.824 kb on - strand, within RR42_RS06245 at 1370.870 kb on + strand, within RR42_RS06245 at 1370.891 kb on + strand at 1370.891 kb on + strand at 1370.892 kb on - strand at 1370.892 kb on - strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.894 kb on - strand at 1370.894 kb on - strand at 1370.894 kb on - strand at 1371.417 kb on + strand, within RR42_RS06250 at 1371.417 kb on + strand, within RR42_RS06250 at 1371.495 kb on + strand, within RR42_RS06250 at 1371.648 kb on + strand, within RR42_RS06250 at 1371.648 kb on + strand, within RR42_RS06250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,370,646 + RR42_RS06245 0.49 +0.1 1,370,647 - RR42_RS06245 0.49 -0.7 1,370,824 - RR42_RS06245 0.79 -2.0 1,370,870 + RR42_RS06245 0.87 -2.8 1,370,891 + +0.0 1,370,891 + -0.0 1,370,892 - -1.0 1,370,892 - -2.4 1,370,893 + -1.6 1,370,893 + +0.2 1,370,893 + -0.3 1,370,893 + +0.1 1,370,893 + +1.7 1,370,894 - -3.6 1,370,894 - -1.5 1,370,894 - -1.6 1,371,417 + RR42_RS06250 0.37 -0.6 1,371,417 + RR42_RS06250 0.37 -2.2 1,371,495 + RR42_RS06250 0.45 -0.0 1,371,648 + RR42_RS06250 0.63 -0.4 1,371,648 + RR42_RS06250 0.63 -2.0
Or see this region's nucleotide sequence