Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05950 and RR42_RS05955 are separated by 202 nucleotides RR42_RS05955 and RR42_RS05960 are separated by 186 nucleotides RR42_RS05960 and RR42_RS05965 are separated by 84 nucleotides RR42_RS05965 and RR42_RS05970 are separated by 72 nucleotides
RR42_RS05950: RR42_RS05950 - phospholipase A, at 1,309,239 to 1,310,471
_RS05950
RR42_RS05955: RR42_RS05955 - hypothetical protein, at 1,310,674 to 1,311,105
_RS05955
RR42_RS05960: RR42_RS05960 - multidrug DMT transporter permease, at 1,311,292 to 1,312,194
_RS05960
RR42_RS05965: RR42_RS05965 - malonic semialdehyde reductase, at 1,312,279 to 1,312,866
_RS05965
RR42_RS05970: RR42_RS05970 - endonuclease III, at 1,312,939 to 1,313,583
_RS05970
Position (kb)
1311
1312
1313 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1310.579 kb on - strand at 1310.598 kb on + strand at 1310.598 kb on + strand at 1310.598 kb on + strand at 1310.598 kb on + strand at 1310.599 kb on - strand at 1310.599 kb on - strand at 1310.599 kb on - strand at 1310.609 kb on + strand at 1310.609 kb on + strand at 1310.609 kb on + strand at 1310.610 kb on - strand at 1310.642 kb on + strand at 1310.695 kb on + strand at 1310.708 kb on + strand at 1310.933 kb on + strand, within RR42_RS05955 at 1311.120 kb on - strand at 1311.192 kb on + strand at 1311.192 kb on + strand at 1311.195 kb on + strand at 1311.195 kb on + strand at 1311.196 kb on - strand at 1311.196 kb on - strand at 1311.385 kb on + strand, within RR42_RS05960 at 1311.407 kb on - strand, within RR42_RS05960 at 1311.532 kb on + strand, within RR42_RS05960 at 1311.532 kb on + strand, within RR42_RS05960 at 1311.533 kb on - strand, within RR42_RS05960 at 1311.533 kb on - strand, within RR42_RS05960 at 1311.604 kb on + strand, within RR42_RS05960 at 1311.604 kb on + strand, within RR42_RS05960 at 1311.605 kb on - strand, within RR42_RS05960 at 1311.605 kb on - strand, within RR42_RS05960 at 1311.605 kb on - strand, within RR42_RS05960 at 1311.775 kb on + strand, within RR42_RS05960 at 1311.776 kb on - strand, within RR42_RS05960 at 1311.784 kb on + strand, within RR42_RS05960 at 1311.785 kb on - strand, within RR42_RS05960 at 1311.785 kb on - strand, within RR42_RS05960 at 1311.868 kb on + strand, within RR42_RS05960 at 1311.869 kb on - strand, within RR42_RS05960 at 1311.869 kb on - strand, within RR42_RS05960 at 1311.962 kb on + strand, within RR42_RS05960 at 1312.236 kb on + strand at 1312.237 kb on - strand at 1312.237 kb on - strand at 1312.237 kb on - strand at 1312.240 kb on + strand at 1312.240 kb on + strand at 1312.240 kb on + strand at 1312.241 kb on - strand at 1312.241 kb on - strand at 1312.241 kb on - strand at 1312.329 kb on + strand at 1312.329 kb on + strand at 1312.330 kb on - strand at 1312.667 kb on - strand, within RR42_RS05965 at 1312.889 kb on - strand at 1312.889 kb on - strand at 1312.889 kb on - strand at 1312.970 kb on + strand at 1313.030 kb on + strand, within RR42_RS05970 at 1313.030 kb on + strand, within RR42_RS05970 at 1313.031 kb on - strand, within RR42_RS05970 at 1313.163 kb on + strand, within RR42_RS05970 at 1313.163 kb on + strand at 1313.163 kb on + strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970 at 1313.164 kb on - strand, within RR42_RS05970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,310,579 - +0.4 1,310,598 + +0.8 1,310,598 + +1.5 1,310,598 + -0.7 1,310,598 + -1.5 1,310,599 - -0.2 1,310,599 - +0.7 1,310,599 - +1.7 1,310,609 + -0.3 1,310,609 + +0.6 1,310,609 + +0.2 1,310,610 - -2.2 1,310,642 + -1.0 1,310,695 + +0.7 1,310,708 + -0.9 1,310,933 + RR42_RS05955 0.60 +0.2 1,311,120 - -0.2 1,311,192 + +0.0 1,311,192 + -3.0 1,311,195 + +1.4 1,311,195 + -1.1 1,311,196 - +1.2 1,311,196 - +0.4 1,311,385 + RR42_RS05960 0.10 +0.2 1,311,407 - RR42_RS05960 0.13 -0.0 1,311,532 + RR42_RS05960 0.27 +0.0 1,311,532 + RR42_RS05960 0.27 +1.6 1,311,533 - RR42_RS05960 0.27 -1.5 1,311,533 - RR42_RS05960 0.27 +0.7 1,311,604 + RR42_RS05960 0.35 -0.7 1,311,604 + RR42_RS05960 0.35 -1.3 1,311,605 - RR42_RS05960 0.35 +1.0 1,311,605 - RR42_RS05960 0.35 -0.3 1,311,605 - RR42_RS05960 0.35 +0.2 1,311,775 + RR42_RS05960 0.53 -1.8 1,311,776 - RR42_RS05960 0.54 -0.6 1,311,784 + RR42_RS05960 0.54 +1.0 1,311,785 - RR42_RS05960 0.55 +0.3 1,311,785 - RR42_RS05960 0.55 +1.0 1,311,868 + RR42_RS05960 0.64 +0.6 1,311,869 - RR42_RS05960 0.64 -0.2 1,311,869 - RR42_RS05960 0.64 -0.2 1,311,962 + RR42_RS05960 0.74 -1.2 1,312,236 + +0.4 1,312,237 - -0.1 1,312,237 - +0.3 1,312,237 - +1.1 1,312,240 + +0.2 1,312,240 + -0.8 1,312,240 + +0.6 1,312,241 - +0.1 1,312,241 - +3.6 1,312,241 - -0.6 1,312,329 + +1.0 1,312,329 + +1.0 1,312,330 - -0.6 1,312,667 - RR42_RS05965 0.66 +1.4 1,312,889 - +0.1 1,312,889 - +1.6 1,312,889 - +2.3 1,312,970 + -1.0 1,313,030 + RR42_RS05970 0.14 -1.6 1,313,030 + RR42_RS05970 0.14 +0.3 1,313,031 - RR42_RS05970 0.14 -0.0 1,313,163 + RR42_RS05970 0.35 -0.4 1,313,163 + +1.0 1,313,163 + RR42_RS05970 0.35 +0.5 1,313,164 - RR42_RS05970 0.35 +0.8 1,313,164 - RR42_RS05970 0.35 -0.3 1,313,164 - RR42_RS05970 0.35 +0.6
Or see this region's nucleotide sequence