Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05260 and RR42_RS05265 are separated by 150 nucleotides RR42_RS05265 and RR42_RS05270 are separated by 313 nucleotides RR42_RS05270 and RR42_RS05275 are separated by 31 nucleotides
RR42_RS05260: RR42_RS05260 - purine permease, at 1,160,904 to 1,162,310
_RS05260
RR42_RS05265: RR42_RS05265 - GntR family transcriptional regulator, at 1,162,461 to 1,163,204
_RS05265
RR42_RS05270: RR42_RS05270 - 5-hydroxyisourate hydrolase, at 1,163,518 to 1,163,871
_RS05270
RR42_RS05275: RR42_RS05275 - membrane protein, at 1,163,903 to 1,165,108
_RS05275
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1161.694 kb on + strand, within RR42_RS05260 at 1161.695 kb on - strand, within RR42_RS05260 at 1161.760 kb on + strand, within RR42_RS05260 at 1161.974 kb on - strand, within RR42_RS05260 at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.304 kb on + strand at 1162.305 kb on - strand at 1162.305 kb on - strand at 1162.371 kb on - strand at 1162.371 kb on - strand at 1162.371 kb on - strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1163.095 kb on + strand, within RR42_RS05265 at 1163.095 kb on + strand, within RR42_RS05265 at 1163.095 kb on + strand, within RR42_RS05265 at 1163.096 kb on - strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.218 kb on + strand at 1163.219 kb on - strand at 1163.313 kb on + strand at 1163.431 kb on + strand at 1163.432 kb on - strand at 1163.432 kb on - strand at 1163.467 kb on + strand at 1163.468 kb on - strand at 1163.540 kb on + strand at 1163.540 kb on + strand at 1163.698 kb on + strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.815 kb on + strand, within RR42_RS05270 at 1163.857 kb on + strand at 1163.858 kb on - strand at 1163.858 kb on - strand at 1163.869 kb on + strand at 1163.869 kb on + strand at 1163.870 kb on - strand at 1163.912 kb on + strand at 1163.912 kb on + strand at 1163.912 kb on + strand at 1163.913 kb on - strand at 1163.913 kb on - strand at 1163.915 kb on - strand at 1163.915 kb on - strand at 1163.915 kb on - strand at 1163.991 kb on - strand at 1164.092 kb on + strand, within RR42_RS05275 at 1164.107 kb on + strand, within RR42_RS05275 at 1164.153 kb on - strand, within RR42_RS05275 at 1164.164 kb on + strand, within RR42_RS05275 at 1164.164 kb on + strand, within RR42_RS05275 at 1164.165 kb on - strand, within RR42_RS05275 at 1164.165 kb on - strand, within RR42_RS05275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,161,694 + RR42_RS05260 0.56 -0.9 1,161,695 - RR42_RS05260 0.56 -0.6 1,161,760 + RR42_RS05260 0.61 +3.2 1,161,974 - RR42_RS05260 0.76 +0.7 1,162,210 + +1.6 1,162,210 + -0.2 1,162,210 + +0.1 1,162,210 + +0.1 1,162,210 + -1.0 1,162,211 - +0.8 1,162,211 - +1.3 1,162,211 - -0.2 1,162,211 - +0.4 1,162,211 - -0.3 1,162,304 + -0.2 1,162,305 - +1.8 1,162,305 - +0.8 1,162,371 - -1.4 1,162,371 - -1.8 1,162,371 - +0.0 1,162,462 + +1.6 1,162,462 + +1.7 1,162,462 + +0.4 1,162,462 + +0.1 1,162,463 - +0.0 1,162,463 - +1.2 1,162,463 - -1.6 1,162,463 - +0.4 1,163,095 + RR42_RS05265 0.85 -0.1 1,163,095 + RR42_RS05265 0.85 -1.2 1,163,095 + RR42_RS05265 0.85 +0.2 1,163,096 - RR42_RS05265 0.85 -1.1 1,163,107 + RR42_RS05265 0.87 -0.0 1,163,107 + RR42_RS05265 0.87 +0.4 1,163,107 + RR42_RS05265 0.87 -0.7 1,163,108 - RR42_RS05265 0.87 +0.1 1,163,108 - RR42_RS05265 0.87 +0.9 1,163,108 - RR42_RS05265 0.87 -0.4 1,163,108 - RR42_RS05265 0.87 +0.8 1,163,218 + -0.7 1,163,219 - -1.0 1,163,313 + -1.4 1,163,431 + +0.2 1,163,432 - -1.6 1,163,432 - +0.6 1,163,467 + -1.7 1,163,468 - +0.2 1,163,540 + +0.0 1,163,540 + +1.6 1,163,698 + RR42_RS05270 0.51 +0.8 1,163,699 - RR42_RS05270 0.51 -0.0 1,163,699 - RR42_RS05270 0.51 +0.0 1,163,699 - RR42_RS05270 0.51 -2.2 1,163,815 + RR42_RS05270 0.84 -0.1 1,163,857 + -0.4 1,163,858 - -0.6 1,163,858 - -1.5 1,163,869 + -0.1 1,163,869 + -0.6 1,163,870 - -0.2 1,163,912 + +0.0 1,163,912 + -1.6 1,163,912 + +0.5 1,163,913 - +0.5 1,163,913 - -0.1 1,163,915 - -0.1 1,163,915 - -1.3 1,163,915 - +0.4 1,163,991 - +0.6 1,164,092 + RR42_RS05275 0.16 +0.3 1,164,107 + RR42_RS05275 0.17 +0.4 1,164,153 - RR42_RS05275 0.21 +0.8 1,164,164 + RR42_RS05275 0.22 -1.3 1,164,164 + RR42_RS05275 0.22 +1.3 1,164,165 - RR42_RS05275 0.22 +1.2 1,164,165 - RR42_RS05275 0.22 +0.0
Or see this region's nucleotide sequence