Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05245

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05240 and RR42_RS05245 are separated by 101 nucleotidesRR42_RS05245 and RR42_RS05250 are separated by 276 nucleotidesRR42_RS05250 and RR42_RS05255 are separated by 8 nucleotides RR42_RS05240: RR42_RS05240 - LysR family transcriptional regulator, at 1,157,669 to 1,157,914 _RS05240 RR42_RS05245: RR42_RS05245 - porin, at 1,158,016 to 1,159,101 _RS05245 RR42_RS05250: RR42_RS05250 - OHCU decarboxylase, at 1,159,378 to 1,159,896 _RS05250 RR42_RS05255: RR42_RS05255 - allantoinase, at 1,159,905 to 1,160,855 _RS05255 Position (kb) 1158 1159 1160Strain fitness (log2 ratio) -3 -2 -1 0 1at 1157.211 kb on + strandat 1157.211 kb on + strandat 1157.211 kb on + strandat 1157.211 kb on + strandat 1157.212 kb on - strandat 1157.212 kb on - strandat 1157.212 kb on - strandat 1157.212 kb on - strandat 1157.212 kb on - strandat 1157.212 kb on - strandat 1157.217 kb on + strandat 1157.217 kb on + strandat 1157.218 kb on - strandat 1157.218 kb on - strandat 1157.218 kb on - strandat 1157.331 kb on + strandat 1157.331 kb on + strandat 1157.534 kb on - strandat 1157.534 kb on - strandat 1157.538 kb on - strandat 1157.562 kb on + strandat 1157.562 kb on + strandat 1157.749 kb on + strand, within RR42_RS05240at 1157.749 kb on + strand, within RR42_RS05240at 1157.750 kb on - strand, within RR42_RS05240at 1157.804 kb on + strand, within RR42_RS05240at 1157.804 kb on + strand, within RR42_RS05240at 1157.804 kb on + strand, within RR42_RS05240at 1158.080 kb on + strandat 1158.086 kb on + strandat 1158.095 kb on + strandat 1158.096 kb on - strandat 1158.123 kb on - strandat 1158.186 kb on - strand, within RR42_RS05245at 1158.192 kb on - strand, within RR42_RS05245at 1158.204 kb on + strand, within RR42_RS05245at 1158.296 kb on + strand, within RR42_RS05245at 1158.296 kb on + strand, within RR42_RS05245at 1158.297 kb on - strand, within RR42_RS05245at 1158.335 kb on + strand, within RR42_RS05245at 1158.335 kb on + strand, within RR42_RS05245at 1158.336 kb on - strand, within RR42_RS05245at 1158.336 kb on - strand, within RR42_RS05245at 1158.336 kb on - strand, within RR42_RS05245at 1158.474 kb on - strand, within RR42_RS05245at 1158.474 kb on - strand, within RR42_RS05245at 1158.474 kb on - strand, within RR42_RS05245at 1158.474 kb on - strand, within RR42_RS05245at 1158.534 kb on - strand, within RR42_RS05245at 1158.575 kb on + strand, within RR42_RS05245at 1158.576 kb on - strand, within RR42_RS05245at 1158.650 kb on + strand, within RR42_RS05245at 1158.680 kb on + strand, within RR42_RS05245at 1158.680 kb on + strand, within RR42_RS05245at 1158.680 kb on + strand, within RR42_RS05245at 1158.680 kb on + strand, within RR42_RS05245at 1158.680 kb on + strand, within RR42_RS05245at 1158.681 kb on - strand, within RR42_RS05245at 1158.681 kb on - strand, within RR42_RS05245at 1158.722 kb on + strand, within RR42_RS05245at 1158.722 kb on + strand, within RR42_RS05245at 1158.914 kb on + strand, within RR42_RS05245at 1158.947 kb on + strand, within RR42_RS05245at 1158.947 kb on + strand, within RR42_RS05245at 1158.947 kb on + strand, within RR42_RS05245at 1159.008 kb on - strandat 1159.096 kb on - strandat 1159.141 kb on + strandat 1159.163 kb on - strandat 1159.165 kb on + strandat 1159.165 kb on + strandat 1159.299 kb on + strandat 1159.321 kb on + strandat 1159.390 kb on + strandat 1159.390 kb on + strandat 1159.391 kb on - strandat 1159.391 kb on - strandat 1159.391 kb on - strandat 1159.391 kb on - strandat 1159.391 kb on - strandat 1159.554 kb on - strand, within RR42_RS05250at 1159.820 kb on + strand, within RR42_RS05250at 1159.820 kb on + strand, within RR42_RS05250at 1159.820 kb on + strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.821 kb on - strand, within RR42_RS05250at 1159.883 kb on + strandat 1159.883 kb on + strandat 1159.884 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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1,157,211 + +1.4
1,157,211 + +0.8
1,157,211 + -0.0
1,157,211 + -0.3
1,157,212 - +0.6
1,157,212 - +1.2
1,157,212 - -0.0
1,157,212 - -1.3
1,157,212 - +0.0
1,157,212 - +0.4
1,157,217 + +0.3
1,157,217 + +0.3
1,157,218 - -0.7
1,157,218 - -0.8
1,157,218 - +1.2
1,157,331 + +1.2
1,157,331 + -1.9
1,157,534 - +0.4
1,157,534 - +0.1
1,157,538 - -2.0
1,157,562 + +0.0
1,157,562 + +1.3
1,157,749 + RR42_RS05240 0.33 +0.5
1,157,749 + RR42_RS05240 0.33 -0.4
1,157,750 - RR42_RS05240 0.33 -0.5
1,157,804 + RR42_RS05240 0.55 +1.1
1,157,804 + RR42_RS05240 0.55 +0.1
1,157,804 + RR42_RS05240 0.55 -0.3
1,158,080 + -0.6
1,158,086 + -1.0
1,158,095 + +0.0
1,158,096 - -0.5
1,158,123 - +0.4
1,158,186 - RR42_RS05245 0.16 +0.2
1,158,192 - RR42_RS05245 0.16 -1.0
1,158,204 + RR42_RS05245 0.17 +0.8
1,158,296 + RR42_RS05245 0.26 +0.0
1,158,296 + RR42_RS05245 0.26 +0.9
1,158,297 - RR42_RS05245 0.26 -1.7
1,158,335 + RR42_RS05245 0.29 -0.9
1,158,335 + RR42_RS05245 0.29 +1.3
1,158,336 - RR42_RS05245 0.29 +1.3
1,158,336 - RR42_RS05245 0.29 +0.2
1,158,336 - RR42_RS05245 0.29 -0.1
1,158,474 - RR42_RS05245 0.42 -2.5
1,158,474 - RR42_RS05245 0.42 -2.8
1,158,474 - RR42_RS05245 0.42 -2.7
1,158,474 - RR42_RS05245 0.42 -0.2
1,158,534 - RR42_RS05245 0.48 +0.6
1,158,575 + RR42_RS05245 0.51 -1.8
1,158,576 - RR42_RS05245 0.52 -2.8
1,158,650 + RR42_RS05245 0.58 -0.7
1,158,680 + RR42_RS05245 0.61 +0.7
1,158,680 + RR42_RS05245 0.61 +0.7
1,158,680 + RR42_RS05245 0.61 -0.0
1,158,680 + RR42_RS05245 0.61 -0.6
1,158,680 + RR42_RS05245 0.61 -0.6
1,158,681 - RR42_RS05245 0.61 -0.1
1,158,681 - RR42_RS05245 0.61 -0.0
1,158,722 + RR42_RS05245 0.65 -0.2
1,158,722 + RR42_RS05245 0.65 +0.3
1,158,914 + RR42_RS05245 0.83 -1.3
1,158,947 + RR42_RS05245 0.86 +1.3
1,158,947 + RR42_RS05245 0.86 -0.4
1,158,947 + RR42_RS05245 0.86 -0.4
1,159,008 - +0.2
1,159,096 - -0.1
1,159,141 + +0.2
1,159,163 - -0.2
1,159,165 + -0.8
1,159,165 + +0.0
1,159,299 + +0.5
1,159,321 + -0.5
1,159,390 + +0.3
1,159,390 + +0.5
1,159,391 - +0.1
1,159,391 - +0.1
1,159,391 - +0.0
1,159,391 - +0.3
1,159,391 - +1.1
1,159,554 - RR42_RS05250 0.34 +1.2
1,159,820 + RR42_RS05250 0.85 +0.5
1,159,820 + RR42_RS05250 0.85 +1.5
1,159,820 + RR42_RS05250 0.85 +0.5
1,159,821 - RR42_RS05250 0.85 -0.8
1,159,821 - RR42_RS05250 0.85 +0.3
1,159,821 - RR42_RS05250 0.85 +0.8
1,159,821 - RR42_RS05250 0.85 +0.7
1,159,821 - RR42_RS05250 0.85 -0.1
1,159,821 - RR42_RS05250 0.85 +0.7
1,159,821 - RR42_RS05250 0.85 +0.3
1,159,883 + +0.0
1,159,883 + +0.8
1,159,884 - +0.5

Or see this region's nucleotide sequence