Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04970 and RR42_RS04975 are separated by 88 nucleotides RR42_RS04975 and RR42_RS04980 are separated by 206 nucleotides RR42_RS04980 and RR42_RS04985 are separated by 172 nucleotides
RR42_RS04970: RR42_RS04970 - aspartyl beta-hydroxylase, at 1,098,115 to 1,099,017
_RS04970
RR42_RS04975: RR42_RS04975 - histidine kinase, at 1,099,106 to 1,100,581
_RS04975
RR42_RS04980: RR42_RS04980 - hypothetical protein, at 1,100,788 to 1,101,189
_RS04980
RR42_RS04985: RR42_RS04985 - 2-aminoadipate aminotransferase, at 1,101,362 to 1,102,804
_RS04985
Position (kb)
1099
1100
1101 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1098.160 kb on + strand at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.254 kb on + strand, within RR42_RS04970 at 1098.255 kb on - strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.428 kb on - strand, within RR42_RS04970 at 1098.445 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on - strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.716 kb on - strand, within RR42_RS04970 at 1098.901 kb on - strand, within RR42_RS04970 at 1098.959 kb on - strand at 1098.961 kb on + strand at 1098.961 kb on + strand at 1098.962 kb on - strand at 1098.962 kb on - strand at 1098.962 kb on - strand at 1099.031 kb on + strand at 1099.032 kb on - strand at 1099.032 kb on - strand at 1099.032 kb on - strand at 1099.146 kb on + strand at 1099.147 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.264 kb on - strand, within RR42_RS04975 at 1099.312 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.344 kb on - strand, within RR42_RS04975 at 1099.344 kb on - strand, within RR42_RS04975 at 1099.552 kb on - strand, within RR42_RS04975 at 1099.555 kb on - strand, within RR42_RS04975 at 1099.567 kb on - strand, within RR42_RS04975 at 1099.567 kb on - strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.609 kb on - strand, within RR42_RS04975 at 1099.609 kb on - strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.811 kb on - strand, within RR42_RS04975 at 1099.811 kb on - strand, within RR42_RS04975 at 1100.067 kb on + strand, within RR42_RS04975 at 1100.068 kb on - strand, within RR42_RS04975 at 1100.068 kb on - strand, within RR42_RS04975 at 1100.214 kb on + strand, within RR42_RS04975 at 1100.214 kb on + strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.290 kb on + strand, within RR42_RS04975 at 1100.291 kb on - strand, within RR42_RS04975 at 1100.318 kb on - strand, within RR42_RS04975 at 1100.544 kb on + strand at 1100.544 kb on + strand at 1100.544 kb on + strand at 1100.545 kb on - strand at 1100.716 kb on + strand at 1100.717 kb on - strand at 1100.717 kb on - strand at 1100.721 kb on + strand at 1100.740 kb on - strand at 1100.740 kb on - strand at 1100.745 kb on + strand at 1100.746 kb on - strand at 1101.000 kb on + strand, within RR42_RS04980 at 1101.001 kb on - strand, within RR42_RS04980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,098,160 + -2.0 1,098,250 + RR42_RS04970 0.15 -4.6 1,098,250 + RR42_RS04970 0.15 -3.4 1,098,250 + RR42_RS04970 0.15 -2.9 1,098,250 + RR42_RS04970 0.15 -2.1 1,098,250 + RR42_RS04970 0.15 -2.6 1,098,251 - RR42_RS04970 0.15 -3.9 1,098,251 - RR42_RS04970 0.15 -3.3 1,098,251 - RR42_RS04970 0.15 -3.8 1,098,251 - RR42_RS04970 0.15 -2.0 1,098,251 - RR42_RS04970 0.15 -4.5 1,098,254 + RR42_RS04970 0.15 -1.0 1,098,255 - RR42_RS04970 0.16 -2.7 1,098,280 + RR42_RS04970 0.18 -3.9 1,098,280 + RR42_RS04970 0.18 -3.0 1,098,280 + RR42_RS04970 0.18 -3.5 1,098,281 - RR42_RS04970 0.18 -0.5 1,098,281 - RR42_RS04970 0.18 -1.3 1,098,281 - RR42_RS04970 0.18 -4.6 1,098,281 - RR42_RS04970 0.18 -3.4 1,098,428 - RR42_RS04970 0.35 -3.0 1,098,445 + RR42_RS04970 0.37 -1.9 1,098,675 + RR42_RS04970 0.62 -2.3 1,098,675 + RR42_RS04970 0.62 -4.0 1,098,675 + RR42_RS04970 0.62 -1.6 1,098,676 - RR42_RS04970 0.62 -2.1 1,098,676 - RR42_RS04970 0.62 -3.3 1,098,676 - RR42_RS04970 0.62 -1.3 1,098,701 + RR42_RS04970 0.65 -3.9 1,098,701 + RR42_RS04970 0.65 -5.0 1,098,701 - RR42_RS04970 0.65 -3.0 1,098,715 + RR42_RS04970 0.66 -2.9 1,098,715 + RR42_RS04970 0.66 -2.0 1,098,716 - RR42_RS04970 0.67 -2.2 1,098,901 - RR42_RS04970 0.87 -0.8 1,098,959 - -2.3 1,098,961 + -5.7 1,098,961 + -3.9 1,098,962 - -3.3 1,098,962 - -5.0 1,098,962 - -4.5 1,099,031 + +0.6 1,099,032 - -1.1 1,099,032 - -0.7 1,099,032 - +0.5 1,099,146 + +0.7 1,099,147 - -2.3 1,099,168 - +1.3 1,099,168 - +0.0 1,099,168 - +0.4 1,099,168 - -0.8 1,099,264 - RR42_RS04975 0.11 -2.2 1,099,312 + RR42_RS04975 0.14 -0.7 1,099,343 + RR42_RS04975 0.16 -0.6 1,099,343 + RR42_RS04975 0.16 +0.0 1,099,343 + RR42_RS04975 0.16 -1.5 1,099,344 - RR42_RS04975 0.16 -0.3 1,099,344 - RR42_RS04975 0.16 -0.0 1,099,552 - RR42_RS04975 0.30 +0.2 1,099,555 - RR42_RS04975 0.30 -1.3 1,099,567 - RR42_RS04975 0.31 -0.2 1,099,567 - RR42_RS04975 0.31 +0.0 1,099,608 + RR42_RS04975 0.34 +0.4 1,099,608 + RR42_RS04975 0.34 +1.0 1,099,608 + RR42_RS04975 0.34 +0.2 1,099,609 - RR42_RS04975 0.34 +0.3 1,099,609 - RR42_RS04975 0.34 +0.6 1,099,706 + RR42_RS04975 0.41 -1.8 1,099,706 + RR42_RS04975 0.41 +0.6 1,099,706 + RR42_RS04975 0.41 +1.2 1,099,706 + RR42_RS04975 0.41 -1.3 1,099,707 - RR42_RS04975 0.41 +0.4 1,099,707 - RR42_RS04975 0.41 +1.0 1,099,707 - RR42_RS04975 0.41 -1.0 1,099,707 - RR42_RS04975 0.41 -0.2 1,099,707 - RR42_RS04975 0.41 +1.0 1,099,707 - RR42_RS04975 0.41 +0.5 1,099,811 - RR42_RS04975 0.48 +0.0 1,099,811 - RR42_RS04975 0.48 -1.5 1,100,067 + RR42_RS04975 0.65 +0.6 1,100,068 - RR42_RS04975 0.65 +0.1 1,100,068 - RR42_RS04975 0.65 -1.6 1,100,214 + RR42_RS04975 0.75 +1.3 1,100,214 + RR42_RS04975 0.75 +1.7 1,100,215 - RR42_RS04975 0.75 -1.0 1,100,215 - RR42_RS04975 0.75 -1.3 1,100,215 - RR42_RS04975 0.75 +0.4 1,100,215 - RR42_RS04975 0.75 +0.8 1,100,215 - RR42_RS04975 0.75 +1.8 1,100,290 + RR42_RS04975 0.80 -0.6 1,100,291 - RR42_RS04975 0.80 +0.1 1,100,318 - RR42_RS04975 0.82 -0.3 1,100,544 + -0.2 1,100,544 + +0.3 1,100,544 + +1.0 1,100,545 - +0.3 1,100,716 + +0.2 1,100,717 - -1.1 1,100,717 - +0.0 1,100,721 + -1.6 1,100,740 - -0.2 1,100,740 - -0.1 1,100,745 + -0.0 1,100,746 - +1.8 1,101,000 + RR42_RS04980 0.53 -0.8 1,101,001 - RR42_RS04980 0.53 +0.2
Or see this region's nucleotide sequence