Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04425 and RR42_RS04430 are separated by 770 nucleotides RR42_RS04430 and RR42_RS04435 are separated by 2 nucleotides RR42_RS04435 and RR42_RS04440 are separated by 111 nucleotides
RR42_RS04425: RR42_RS04425 - hypothetical protein, at 988,014 to 988,877
_RS04425
RR42_RS04430: RR42_RS04430 - phosphoglycolate phosphatase, at 989,648 to 990,319
_RS04430
RR42_RS04435: RR42_RS04435 - 3-demethylubiquinone-9 3-methyltransferase, at 990,322 to 991,071
_RS04435
RR42_RS04440: RR42_RS04440 - membrane protein, at 991,183 to 991,842
_RS04440
Position (kb)
989
990
991 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 988.688 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.881 kb on + strand at 988.881 kb on + strand at 988.882 kb on - strand at 988.951 kb on + strand at 988.963 kb on - strand at 988.963 kb on - strand at 988.963 kb on - strand at 989.078 kb on - strand at 989.078 kb on - strand at 989.126 kb on - strand at 989.126 kb on - strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.150 kb on - strand at 989.270 kb on + strand at 989.271 kb on - strand at 989.313 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.370 kb on - strand at 989.370 kb on - strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.374 kb on - strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.378 kb on - strand at 989.424 kb on + strand at 989.424 kb on + strand at 989.425 kb on - strand at 989.488 kb on + strand at 989.501 kb on + strand at 989.501 kb on + strand at 989.501 kb on + strand at 989.553 kb on - strand at 989.568 kb on - strand at 989.599 kb on - strand at 989.621 kb on + strand at 989.621 kb on + strand at 989.621 kb on + strand at 989.706 kb on + strand at 989.755 kb on - strand, within RR42_RS04430 at 989.807 kb on + strand, within RR42_RS04430 at 989.807 kb on + strand, within RR42_RS04430 at 989.808 kb on - strand, within RR42_RS04430 at 989.808 kb on - strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.824 kb on - strand, within RR42_RS04430 at 989.996 kb on - strand, within RR42_RS04430 at 989.996 kb on - strand, within RR42_RS04430 at 990.061 kb on + strand, within RR42_RS04430 at 990.061 kb on + strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.084 kb on + strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.088 kb on - strand, within RR42_RS04430 at 990.088 kb on - strand, within RR42_RS04430 at 990.309 kb on + strand at 990.310 kb on - strand at 990.310 kb on - strand at 991.128 kb on + strand at 991.129 kb on - strand at 991.184 kb on + strand at 991.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 988,688 + RR42_RS04425 0.78 -1.2 988,690 + RR42_RS04425 0.78 +2.3 988,690 + RR42_RS04425 0.78 +0.5 988,690 + RR42_RS04425 0.78 +0.3 988,690 + RR42_RS04425 0.78 +0.3 988,690 + RR42_RS04425 0.78 -0.2 988,691 - RR42_RS04425 0.78 +0.4 988,691 - RR42_RS04425 0.78 +0.4 988,691 - RR42_RS04425 0.78 -0.7 988,691 - RR42_RS04425 0.78 +0.1 988,691 - RR42_RS04425 0.78 -0.5 988,691 - RR42_RS04425 0.78 -1.0 988,691 - RR42_RS04425 0.78 +0.0 988,691 - RR42_RS04425 0.78 -1.4 988,881 + -0.5 988,881 + +0.6 988,882 - -2.8 988,951 + -0.9 988,963 - +0.7 988,963 - -0.1 988,963 - +1.8 989,078 - -1.4 989,078 - -2.3 989,126 - -0.0 989,126 - -0.2 989,149 + -1.7 989,149 + -1.2 989,149 + -0.7 989,149 + +0.1 989,150 - -0.9 989,270 + +0.5 989,271 - +0.1 989,313 + +0.2 989,369 + -0.3 989,369 + +0.7 989,369 + +0.7 989,369 + -0.8 989,370 - -1.3 989,370 - +0.1 989,373 + -0.7 989,373 + +1.1 989,373 + +0.1 989,373 + -1.3 989,373 + -0.2 989,374 - -0.7 989,377 + +0.5 989,377 + +0.1 989,377 + +1.1 989,377 + +1.0 989,377 + -0.0 989,378 - +0.0 989,424 + +0.7 989,424 + +0.1 989,425 - -0.9 989,488 + -1.5 989,501 + -1.2 989,501 + +0.4 989,501 + +2.1 989,553 - +1.1 989,568 - -1.3 989,599 - +1.1 989,621 + -1.0 989,621 + +0.9 989,621 + +0.9 989,706 + +0.2 989,755 - RR42_RS04430 0.16 +0.8 989,807 + RR42_RS04430 0.24 +0.4 989,807 + RR42_RS04430 0.24 -0.2 989,808 - RR42_RS04430 0.24 +1.1 989,808 - RR42_RS04430 0.24 +0.5 989,823 + RR42_RS04430 0.26 +0.3 989,823 + RR42_RS04430 0.26 +1.3 989,823 + RR42_RS04430 0.26 +1.7 989,823 + RR42_RS04430 0.26 +1.8 989,823 + RR42_RS04430 0.26 +0.7 989,824 - RR42_RS04430 0.26 +0.7 989,996 - RR42_RS04430 0.52 +1.1 989,996 - RR42_RS04430 0.52 +1.4 990,061 + RR42_RS04430 0.61 +0.4 990,061 + RR42_RS04430 0.61 +1.3 990,062 - RR42_RS04430 0.62 +1.1 990,062 - RR42_RS04430 0.62 +1.4 990,062 - RR42_RS04430 0.62 +1.0 990,062 - RR42_RS04430 0.62 +0.4 990,062 - RR42_RS04430 0.62 +0.7 990,084 + RR42_RS04430 0.65 +1.0 990,085 - RR42_RS04430 0.65 +0.7 990,085 - RR42_RS04430 0.65 +1.3 990,085 - RR42_RS04430 0.65 +0.7 990,085 - RR42_RS04430 0.65 +0.3 990,085 - RR42_RS04430 0.65 +0.4 990,085 - RR42_RS04430 0.65 +0.9 990,088 - RR42_RS04430 0.65 +0.6 990,088 - RR42_RS04430 0.65 +0.4 990,309 + +1.4 990,310 - -0.4 990,310 - +0.5 991,128 + +0.8 991,129 - +0.4 991,184 + +0.1 991,185 - -1.9
Or see this region's nucleotide sequence