Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04315 and RR42_RS04320 are separated by 170 nucleotides RR42_RS04320 and RR42_RS04325 are separated by 133 nucleotides RR42_RS04325 and RR42_RS04330 are separated by 23 nucleotides
RR42_RS04315: RR42_RS04315 - thioredoxin reductase, at 957,970 to 958,917
_RS04315
RR42_RS04320: RR42_RS04320 - cell division protein FtsK, at 959,088 to 961,412
_RS04320
RR42_RS04325: RR42_RS04325 - membrane protein, at 961,546 to 962,184
_RS04325
RR42_RS04330: RR42_RS04330 - lipoprotein, at 962,208 to 962,612
_RS04330
Position (kb) 959
960
961
962 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 958.978 kb on + strand at 958.978 kb on + strand at 958.978 kb on + strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 959.041 kb on + strand at 959.042 kb on - strand at 959.042 kb on - strand at 960.196 kb on - strand, within RR42_RS04320 at 960.196 kb on - strand, within RR42_RS04320 at 960.426 kb on + strand, within RR42_RS04320 at 960.426 kb on + strand, within RR42_RS04320 at 960.427 kb on - strand, within RR42_RS04320 at 960.492 kb on + strand, within RR42_RS04320 at 960.492 kb on + strand, within RR42_RS04320 at 960.630 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.110 kb on + strand, within RR42_RS04320 at 961.111 kb on - strand, within RR42_RS04320 at 961.410 kb on + strand at 961.411 kb on - strand at 961.411 kb on - strand at 961.424 kb on + strand at 961.424 kb on + strand at 961.435 kb on + strand at 961.828 kb on + strand, within RR42_RS04325 at 961.828 kb on + strand, within RR42_RS04325 at 961.828 kb on + strand, within RR42_RS04325 at 961.834 kb on + strand, within RR42_RS04325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 958,978 + -0.2 958,978 + -0.3 958,978 + -0.4 958,979 - -0.3 958,979 - +1.0 958,979 - +2.2 958,979 - -0.2 958,979 - -0.9 958,979 - -0.3 959,041 + +0.7 959,042 - -0.1 959,042 - -0.4 960,196 - RR42_RS04320 0.48 -2.4 960,196 - RR42_RS04320 0.48 -2.8 960,426 + RR42_RS04320 0.58 -1.6 960,426 + RR42_RS04320 0.58 -0.4 960,427 - RR42_RS04320 0.58 -1.3 960,492 + RR42_RS04320 0.60 +0.4 960,492 + RR42_RS04320 0.60 -1.3 960,630 + RR42_RS04320 0.66 -1.7 961,002 + RR42_RS04320 0.82 +0.3 961,002 + RR42_RS04320 0.82 +0.6 961,002 + RR42_RS04320 0.82 -1.9 961,110 + RR42_RS04320 0.87 +0.6 961,111 - RR42_RS04320 0.87 -2.7 961,410 + -1.6 961,411 - -2.0 961,411 - -1.9 961,424 + -1.1 961,424 + +1.6 961,435 + -0.1 961,828 + RR42_RS04325 0.44 -2.6 961,828 + RR42_RS04325 0.44 -3.9 961,828 + RR42_RS04325 0.44 -2.4 961,834 + RR42_RS04325 0.45 -3.2
Or see this region's nucleotide sequence