Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04055 and RR42_RS04060 are separated by 593 nucleotides RR42_RS04060 and RR42_RS04065 are separated by 148 nucleotides RR42_RS04065 and RR42_RS04070 overlap by 4 nucleotides
RR42_RS04055: RR42_RS04055 - hypothetical protein, at 898,284 to 898,745
_RS04055
RR42_RS04060: RR42_RS04060 - hypothetical protein, at 899,339 to 899,554
_RS04060
RR42_RS04065: RR42_RS04065 - LysR family transcriptional regulator, at 899,703 to 900,566
_RS04065
RR42_RS04070: RR42_RS04070 - quinone oxidoreductase, at 900,563 to 901,567
_RS04070
Position (kb) 899
900
901 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 898.778 kb on + strand at 898.779 kb on - strand at 898.779 kb on - strand at 898.820 kb on + strand at 898.821 kb on - strand at 898.821 kb on - strand at 898.859 kb on + strand at 898.875 kb on + strand at 899.071 kb on + strand at 899.072 kb on - strand at 899.212 kb on + strand at 899.212 kb on + strand at 899.212 kb on + strand at 899.212 kb on + strand at 899.213 kb on - strand at 899.213 kb on - strand at 899.213 kb on - strand at 899.242 kb on + strand at 899.242 kb on + strand at 899.450 kb on + strand, within RR42_RS04060 at 899.451 kb on - strand, within RR42_RS04060 at 899.457 kb on - strand, within RR42_RS04060 at 899.519 kb on + strand, within RR42_RS04060 at 899.673 kb on + strand at 899.682 kb on + strand at 899.717 kb on + strand at 899.718 kb on - strand at 899.913 kb on - strand, within RR42_RS04065 at 900.179 kb on + strand, within RR42_RS04065 at 900.180 kb on - strand, within RR42_RS04065 at 900.186 kb on + strand, within RR42_RS04065 at 900.187 kb on + strand, within RR42_RS04065 at 900.480 kb on + strand at 900.564 kb on + strand at 900.780 kb on + strand, within RR42_RS04070 at 900.780 kb on + strand, within RR42_RS04070 at 900.780 kb on + strand, within RR42_RS04070 at 900.780 kb on + strand, within RR42_RS04070 at 900.856 kb on - strand, within RR42_RS04070 at 900.856 kb on - strand, within RR42_RS04070 at 900.909 kb on + strand, within RR42_RS04070 at 900.910 kb on - strand, within RR42_RS04070 at 900.939 kb on + strand, within RR42_RS04070 at 900.939 kb on + strand, within RR42_RS04070 at 900.939 kb on + strand, within RR42_RS04070 at 900.939 kb on + strand, within RR42_RS04070 at 900.940 kb on - strand, within RR42_RS04070 at 900.940 kb on - strand, within RR42_RS04070 at 900.940 kb on - strand, within RR42_RS04070 at 900.940 kb on - strand, within RR42_RS04070 at 900.951 kb on + strand, within RR42_RS04070 at 900.952 kb on - strand, within RR42_RS04070 at 900.952 kb on - strand, within RR42_RS04070 at 900.996 kb on + strand, within RR42_RS04070 at 901.172 kb on + strand, within RR42_RS04070 at 901.172 kb on + strand, within RR42_RS04070 at 901.173 kb on - strand, within RR42_RS04070 at 901.173 kb on - strand, within RR42_RS04070 at 901.299 kb on + strand, within RR42_RS04070 at 901.300 kb on - strand, within RR42_RS04070 at 901.300 kb on - strand, within RR42_RS04070 at 901.300 kb on - strand, within RR42_RS04070 at 901.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 898,778 + +1.1 898,779 - +1.3 898,779 - +1.1 898,820 + +0.5 898,821 - +0.2 898,821 - +0.8 898,859 + +0.1 898,875 + +1.3 899,071 + -0.3 899,072 - -1.7 899,212 + +0.7 899,212 + +0.7 899,212 + -0.5 899,212 + +0.4 899,213 - +1.5 899,213 - +0.8 899,213 - +0.9 899,242 + +1.4 899,242 + -1.3 899,450 + RR42_RS04060 0.51 -1.6 899,451 - RR42_RS04060 0.52 -0.4 899,457 - RR42_RS04060 0.55 -0.2 899,519 + RR42_RS04060 0.83 -0.3 899,673 + +0.6 899,682 + -0.7 899,717 + -0.5 899,718 - +0.5 899,913 - RR42_RS04065 0.24 -0.9 900,179 + RR42_RS04065 0.55 -0.7 900,180 - RR42_RS04065 0.55 -0.6 900,186 + RR42_RS04065 0.56 -0.8 900,187 + RR42_RS04065 0.56 +0.1 900,480 + -0.0 900,564 + +0.1 900,780 + RR42_RS04070 0.22 +0.5 900,780 + RR42_RS04070 0.22 -1.1 900,780 + RR42_RS04070 0.22 +0.6 900,780 + RR42_RS04070 0.22 +0.3 900,856 - RR42_RS04070 0.29 +1.2 900,856 - RR42_RS04070 0.29 -0.9 900,909 + RR42_RS04070 0.34 -0.4 900,910 - RR42_RS04070 0.35 +0.8 900,939 + RR42_RS04070 0.37 -1.2 900,939 + RR42_RS04070 0.37 +0.1 900,939 + RR42_RS04070 0.37 +0.0 900,939 + RR42_RS04070 0.37 +0.1 900,940 - RR42_RS04070 0.38 +0.5 900,940 - RR42_RS04070 0.38 -0.5 900,940 - RR42_RS04070 0.38 -1.5 900,940 - RR42_RS04070 0.38 +0.3 900,951 + RR42_RS04070 0.39 +0.2 900,952 - RR42_RS04070 0.39 +0.3 900,952 - RR42_RS04070 0.39 +0.1 900,996 + RR42_RS04070 0.43 -0.1 901,172 + RR42_RS04070 0.61 -2.4 901,172 + RR42_RS04070 0.61 +0.4 901,173 - RR42_RS04070 0.61 -3.2 901,173 - RR42_RS04070 0.61 +2.1 901,299 + RR42_RS04070 0.73 +0.3 901,300 - RR42_RS04070 0.73 +0.4 901,300 - RR42_RS04070 0.73 -0.5 901,300 - RR42_RS04070 0.73 -1.0 901,470 - +0.3
Or see this region's nucleotide sequence