Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02785 and RR42_RS02790 are separated by 158 nucleotides RR42_RS02790 and RR42_RS02795 are separated by 766 nucleotides
RR42_RS02785: RR42_RS02785 - dimethyladenosine transferase, at 620,493 to 621,314
_RS02785
RR42_RS02790: RR42_RS02790 - tryptophanyl-tRNA synthetase, at 621,473 to 622,495
_RS02790
RR42_RS02795: RR42_RS02795 - multidrug DMT transporter permease, at 623,262 to 624,170
_RS02795
Position (kb) 621
622
623 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 620.488 kb on + strand at 620.488 kb on + strand at 620.489 kb on - strand at 620.489 kb on - strand at 620.492 kb on + strand at 620.492 kb on + strand at 620.506 kb on + strand at 620.985 kb on + strand, within RR42_RS02785 at 620.985 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.186 kb on - strand, within RR42_RS02785 at 621.274 kb on - strand at 621.308 kb on + strand at 621.309 kb on - strand at 621.458 kb on - strand at 621.554 kb on + strand at 621.555 kb on - strand at 621.555 kb on - strand at 621.605 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.222 kb on + strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.227 kb on - strand, within RR42_RS02790 at 622.450 kb on + strand at 622.451 kb on - strand at 622.487 kb on - strand at 622.487 kb on - strand at 622.550 kb on - strand at 622.962 kb on - strand at 623.198 kb on + strand at 623.416 kb on + strand, within RR42_RS02795 at 623.417 kb on - strand, within RR42_RS02795 at 623.428 kb on - strand, within RR42_RS02795 at 623.446 kb on + strand, within RR42_RS02795 at 623.446 kb on + strand, within RR42_RS02795 at 623.447 kb on - strand, within RR42_RS02795 at 623.477 kb on - strand, within RR42_RS02795 at 623.477 kb on - strand, within RR42_RS02795 at 623.477 kb on - strand, within RR42_RS02795
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 620,488 + +1.1 620,488 + -1.7 620,489 - -2.0 620,489 - -1.1 620,492 + +0.7 620,492 + -0.5 620,506 + -2.5 620,985 + RR42_RS02785 0.60 -2.5 620,985 + RR42_RS02785 0.60 -2.9 621,185 + RR42_RS02785 0.84 -0.4 621,185 + RR42_RS02785 0.84 -0.8 621,186 - RR42_RS02785 0.84 -1.4 621,274 - -1.8 621,308 + +0.9 621,309 - -2.0 621,458 - -1.0 621,554 + -1.0 621,555 - +0.5 621,555 - -1.6 621,605 + RR42_RS02790 0.13 -0.4 621,779 + RR42_RS02790 0.30 +0.6 621,779 + RR42_RS02790 0.30 +1.2 621,779 + RR42_RS02790 0.30 -0.9 621,779 + RR42_RS02790 0.30 +0.2 622,160 + RR42_RS02790 0.67 -0.6 622,160 + RR42_RS02790 0.67 -0.2 622,222 + RR42_RS02790 0.73 -1.0 622,223 - RR42_RS02790 0.73 +0.5 622,223 - RR42_RS02790 0.73 -0.7 622,223 - RR42_RS02790 0.73 -0.4 622,227 - RR42_RS02790 0.74 -0.6 622,450 + -2.4 622,451 - -0.6 622,487 - -1.4 622,487 - -0.9 622,550 - -1.1 622,962 - +0.9 623,198 + +0.9 623,416 + RR42_RS02795 0.17 -0.3 623,417 - RR42_RS02795 0.17 -0.4 623,428 - RR42_RS02795 0.18 -0.0 623,446 + RR42_RS02795 0.20 -0.1 623,446 + RR42_RS02795 0.20 -0.1 623,447 - RR42_RS02795 0.20 +1.9 623,477 - RR42_RS02795 0.24 +0.7 623,477 - RR42_RS02795 0.24 -2.4 623,477 - RR42_RS02795 0.24 -2.6
Or see this region's nucleotide sequence