Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02700 and RR42_RS02705 are separated by 127 nucleotides RR42_RS02705 and RR42_RS02710 are separated by 159 nucleotides RR42_RS02710 and RR42_RS02715 are separated by 132 nucleotides
RR42_RS02700: RR42_RS02700 - ATP-dependent DNA helicase RuvB, at 601,811 to 602,866
_RS02700
RR42_RS02705: RR42_RS02705 - Holliday junction DNA helicase RuvA, at 602,994 to 603,575
_RS02705
RR42_RS02710: RR42_RS02710 - glycerophosphodiester phosphodiesterase, at 603,735 to 604,868
_RS02710
RR42_RS02715: RR42_RS02715 - Holliday junction DNA helicase, at 605,001 to 605,546
_RS02715
Position (kb) 603
604
605 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 602.869 kb on + strand at 602.870 kb on - strand at 602.898 kb on - strand at 603.470 kb on - strand, within RR42_RS02705 at 603.581 kb on - strand at 603.581 kb on - strand at 603.581 kb on - strand at 603.581 kb on - strand at 603.581 kb on - strand at 603.581 kb on - strand at 603.581 kb on - strand at 603.679 kb on + strand at 603.679 kb on + strand at 603.680 kb on - strand at 603.680 kb on - strand at 603.680 kb on - strand at 603.697 kb on - strand at 603.749 kb on + strand at 603.914 kb on + strand, within RR42_RS02710 at 603.914 kb on + strand, within RR42_RS02710 at 603.914 kb on + strand, within RR42_RS02710 at 603.914 kb on + strand, within RR42_RS02710 at 603.915 kb on - strand, within RR42_RS02710 at 603.916 kb on + strand, within RR42_RS02710 at 603.916 kb on + strand, within RR42_RS02710 at 603.916 kb on + strand, within RR42_RS02710 at 603.916 kb on + strand, within RR42_RS02710 at 603.917 kb on - strand, within RR42_RS02710 at 604.036 kb on + strand, within RR42_RS02710 at 604.045 kb on - strand, within RR42_RS02710 at 604.081 kb on + strand, within RR42_RS02710 at 604.246 kb on + strand, within RR42_RS02710 at 604.247 kb on - strand, within RR42_RS02710 at 604.247 kb on - strand, within RR42_RS02710 at 604.247 kb on - strand, within RR42_RS02710 at 604.247 kb on - strand, within RR42_RS02710 at 604.309 kb on + strand, within RR42_RS02710 at 604.309 kb on + strand, within RR42_RS02710 at 604.423 kb on + strand, within RR42_RS02710 at 604.424 kb on - strand, within RR42_RS02710 at 604.424 kb on - strand, within RR42_RS02710 at 604.424 kb on - strand, within RR42_RS02710 at 604.623 kb on + strand, within RR42_RS02710 at 604.623 kb on + strand, within RR42_RS02710 at 604.624 kb on - strand, within RR42_RS02710 at 604.955 kb on + strand at 604.955 kb on + strand at 604.955 kb on + strand at 605.587 kb on + strand at 605.587 kb on + strand at 605.587 kb on + strand at 605.587 kb on + strand at 605.588 kb on - strand at 605.588 kb on - strand at 605.588 kb on - strand at 605.602 kb on - strand at 605.608 kb on + strand at 605.609 kb on - strand at 605.614 kb on + strand at 605.615 kb on - strand at 605.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 602,869 + -2.9 602,870 - -1.8 602,898 - +0.7 603,470 - RR42_RS02705 0.82 -3.5 603,581 - +0.1 603,581 - -0.3 603,581 - +0.2 603,581 - +1.0 603,581 - +1.6 603,581 - -0.1 603,581 - +2.8 603,679 + -1.1 603,679 + +0.3 603,680 - +0.9 603,680 - -0.8 603,680 - -0.4 603,697 - +0.1 603,749 + +0.4 603,914 + RR42_RS02710 0.16 +0.4 603,914 + RR42_RS02710 0.16 -1.0 603,914 + RR42_RS02710 0.16 +0.9 603,914 + RR42_RS02710 0.16 +0.4 603,915 - RR42_RS02710 0.16 +0.9 603,916 + RR42_RS02710 0.16 +0.8 603,916 + RR42_RS02710 0.16 +0.9 603,916 + RR42_RS02710 0.16 -0.3 603,916 + RR42_RS02710 0.16 +0.4 603,917 - RR42_RS02710 0.16 -1.6 604,036 + RR42_RS02710 0.27 -0.2 604,045 - RR42_RS02710 0.27 +1.2 604,081 + RR42_RS02710 0.31 +0.1 604,246 + RR42_RS02710 0.45 +1.0 604,247 - RR42_RS02710 0.45 +0.5 604,247 - RR42_RS02710 0.45 +0.5 604,247 - RR42_RS02710 0.45 +1.6 604,247 - RR42_RS02710 0.45 -0.0 604,309 + RR42_RS02710 0.51 +1.2 604,309 + RR42_RS02710 0.51 +0.7 604,423 + RR42_RS02710 0.61 -1.7 604,424 - RR42_RS02710 0.61 +0.6 604,424 - RR42_RS02710 0.61 +0.3 604,424 - RR42_RS02710 0.61 +0.4 604,623 + RR42_RS02710 0.78 +0.3 604,623 + RR42_RS02710 0.78 +0.9 604,624 - RR42_RS02710 0.78 -0.1 604,955 + +0.3 604,955 + +0.5 604,955 + -0.6 605,587 + -0.7 605,587 + -0.5 605,587 + +0.1 605,587 + -1.1 605,588 - -0.3 605,588 - +0.9 605,588 - -2.8 605,602 - +1.3 605,608 + -0.9 605,609 - -0.9 605,614 + -1.3 605,615 - +0.5 605,615 - -0.5
Or see this region's nucleotide sequence