Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02520 and RR42_RS02525 are separated by 54 nucleotides RR42_RS02525 and RR42_RS02530 are separated by 26 nucleotides RR42_RS02530 and RR42_RS02535 are separated by 177 nucleotides
RR42_RS02520: RR42_RS02520 - hypothetical protein, at 567,678 to 568,634
_RS02520
RR42_RS02525: RR42_RS02525 - enoyl-CoA hydratase, at 568,689 to 569,465
_RS02525
RR42_RS02530: RR42_RS02530 - acyl-CoA hydrolase, at 569,492 to 569,914
_RS02530
RR42_RS02535: RR42_RS02535 - metal ABC transporter permease, at 570,092 to 571,987
_RS02535
Position (kb) 569
570 Strain fitness (log2 ratio) -2
-1
0
1 at 568.494 kb on + strand, within RR42_RS02520 at 568.495 kb on - strand, within RR42_RS02520 at 568.602 kb on + strand at 568.603 kb on - strand at 568.603 kb on - strand at 568.777 kb on - strand, within RR42_RS02525 at 568.777 kb on - strand, within RR42_RS02525 at 568.936 kb on + strand, within RR42_RS02525 at 568.936 kb on + strand, within RR42_RS02525 at 568.937 kb on - strand, within RR42_RS02525 at 569.046 kb on + strand, within RR42_RS02525 at 569.046 kb on + strand, within RR42_RS02525 at 569.047 kb on - strand, within RR42_RS02525 at 569.047 kb on - strand, within RR42_RS02525 at 569.047 kb on - strand, within RR42_RS02525 at 569.276 kb on - strand, within RR42_RS02525 at 569.464 kb on - strand at 569.464 kb on - strand at 569.464 kb on - strand at 569.542 kb on + strand, within RR42_RS02530 at 569.542 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.560 kb on - strand, within RR42_RS02530 at 569.619 kb on + strand, within RR42_RS02530 at 569.620 kb on - strand, within RR42_RS02530 at 569.633 kb on + strand, within RR42_RS02530 at 570.042 kb on + strand at 570.046 kb on + strand at 570.046 kb on + strand at 570.071 kb on + strand at 570.091 kb on + strand at 570.101 kb on + strand at 570.102 kb on - strand at 570.106 kb on + strand at 570.106 kb on + strand at 570.107 kb on - strand at 570.107 kb on - strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.216 kb on - strand at 570.216 kb on - strand at 570.593 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.659 kb on - strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.700 kb on + strand, within RR42_RS02535 at 570.701 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 568,494 + RR42_RS02520 0.85 +0.2 568,495 - RR42_RS02520 0.85 -0.2 568,602 + -1.1 568,603 - +0.0 568,603 - -0.3 568,777 - RR42_RS02525 0.11 -2.0 568,777 - RR42_RS02525 0.11 -1.0 568,936 + RR42_RS02525 0.32 +0.4 568,936 + RR42_RS02525 0.32 +1.0 568,937 - RR42_RS02525 0.32 -0.5 569,046 + RR42_RS02525 0.46 +0.6 569,046 + RR42_RS02525 0.46 +0.1 569,047 - RR42_RS02525 0.46 +0.2 569,047 - RR42_RS02525 0.46 -2.1 569,047 - RR42_RS02525 0.46 -1.3 569,276 - RR42_RS02525 0.76 +0.4 569,464 - -0.1 569,464 - +0.3 569,464 - +0.3 569,542 + RR42_RS02530 0.12 +0.6 569,542 + RR42_RS02530 0.12 -0.3 569,559 + RR42_RS02530 0.16 +1.4 569,559 + RR42_RS02530 0.16 -0.4 569,559 + RR42_RS02530 0.16 +1.3 569,559 + RR42_RS02530 0.16 +0.1 569,560 - RR42_RS02530 0.16 +0.1 569,619 + RR42_RS02530 0.30 -0.6 569,620 - RR42_RS02530 0.30 -0.2 569,633 + RR42_RS02530 0.33 +1.2 570,042 + +0.5 570,046 + -0.5 570,046 + -1.7 570,071 + -0.1 570,091 + -0.2 570,101 + -0.0 570,102 - +0.4 570,106 + -0.0 570,106 + -1.5 570,107 - +0.2 570,107 - +0.4 570,203 + +1.1 570,203 + -0.0 570,203 + -0.4 570,216 - +0.8 570,216 - -0.3 570,593 + RR42_RS02535 0.26 -1.1 570,653 + RR42_RS02535 0.30 +1.0 570,653 + RR42_RS02535 0.30 -0.9 570,653 + RR42_RS02535 0.30 -0.1 570,659 - RR42_RS02535 0.30 -1.8 570,698 + RR42_RS02535 0.32 -0.0 570,698 + RR42_RS02535 0.32 -0.1 570,698 + RR42_RS02535 0.32 -0.4 570,699 - RR42_RS02535 0.32 +0.3 570,699 - RR42_RS02535 0.32 +0.8 570,699 - RR42_RS02535 0.32 -2.2 570,699 - RR42_RS02535 0.32 +0.1 570,699 - RR42_RS02535 0.32 +0.1 570,699 - RR42_RS02535 0.32 -0.6 570,700 + RR42_RS02535 0.32 +0.9 570,701 - RR42_RS02535 0.32 -0.8 570,840 - RR42_RS02535 0.39 +0.8 570,840 - RR42_RS02535 0.39 -0.5
Or see this region's nucleotide sequence