Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02510 and RR42_RS02515 are separated by 18 nucleotides RR42_RS02515 and RR42_RS02520 are separated by 144 nucleotides
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
RR42_RS02515: RR42_RS02515 - acetyl-CoA acetyltransferase, at 566,334 to 567,533
_RS02515
RR42_RS02520: RR42_RS02520 - hypothetical protein, at 567,678 to 568,634
_RS02520
Position (kb) 566
567
568 Strain fitness (log2 ratio) -1
0
1
2 at 565.340 kb on - strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.738 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.359 kb on - strand at 566.359 kb on - strand at 567.469 kb on - strand at 567.515 kb on + strand at 567.575 kb on + strand at 567.596 kb on + strand at 567.621 kb on - strand at 567.621 kb on - strand at 567.828 kb on + strand, within RR42_RS02520 at 567.828 kb on + strand, within RR42_RS02520 at 567.978 kb on + strand, within RR42_RS02520 at 567.979 kb on - strand, within RR42_RS02520 at 567.979 kb on - strand, within RR42_RS02520 at 568.202 kb on + strand, within RR42_RS02520 at 568.202 kb on + strand, within RR42_RS02520 at 568.203 kb on - strand, within RR42_RS02520 at 568.230 kb on + strand, within RR42_RS02520 at 568.231 kb on - strand, within RR42_RS02520 at 568.236 kb on + strand, within RR42_RS02520 at 568.236 kb on + strand, within RR42_RS02520 at 568.237 kb on - strand, within RR42_RS02520 at 568.284 kb on + strand, within RR42_RS02520 at 568.326 kb on - strand, within RR42_RS02520 at 568.326 kb on - strand, within RR42_RS02520 at 568.455 kb on + strand, within RR42_RS02520 at 568.455 kb on + strand, within RR42_RS02520 at 568.494 kb on + strand, within RR42_RS02520 at 568.495 kb on - strand, within RR42_RS02520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 565,340 - RR42_RS02510 0.60 +1.8 565,340 - RR42_RS02510 0.60 +0.2 565,635 + RR42_RS02510 0.72 +0.1 565,635 + RR42_RS02510 0.72 -1.0 565,737 + RR42_RS02510 0.76 +0.7 565,737 + RR42_RS02510 0.76 -0.3 565,737 + RR42_RS02510 0.76 -0.0 565,737 + RR42_RS02510 0.76 +0.9 565,738 - RR42_RS02510 0.76 +0.4 565,846 - RR42_RS02510 0.81 +0.6 565,846 - RR42_RS02510 0.81 -0.5 566,358 + +0.4 566,358 + +1.6 566,358 + +0.1 566,358 + +0.8 566,358 + -1.6 566,359 - +0.4 566,359 - +0.7 567,469 - +0.1 567,515 + -0.5 567,575 + +0.2 567,596 + -0.1 567,621 - -0.7 567,621 - +0.3 567,828 + RR42_RS02520 0.16 -0.7 567,828 + RR42_RS02520 0.16 +0.8 567,978 + RR42_RS02520 0.31 +0.2 567,979 - RR42_RS02520 0.31 +0.1 567,979 - RR42_RS02520 0.31 -0.6 568,202 + RR42_RS02520 0.55 +0.6 568,202 + RR42_RS02520 0.55 -1.6 568,203 - RR42_RS02520 0.55 +0.2 568,230 + RR42_RS02520 0.58 +0.8 568,231 - RR42_RS02520 0.58 -1.0 568,236 + RR42_RS02520 0.58 +0.6 568,236 + RR42_RS02520 0.58 +0.1 568,237 - RR42_RS02520 0.58 +0.3 568,284 + RR42_RS02520 0.63 -0.5 568,326 - RR42_RS02520 0.68 -0.1 568,326 - RR42_RS02520 0.68 -0.4 568,455 + RR42_RS02520 0.81 +0.0 568,455 + RR42_RS02520 0.81 -0.7 568,494 + RR42_RS02520 0.85 +0.2 568,495 - RR42_RS02520 0.85 -0.2
Or see this region's nucleotide sequence