Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02505 and RR42_RS02510 are separated by 195 nucleotides RR42_RS02510 and RR42_RS02515 are separated by 18 nucleotides
RR42_RS02505: RR42_RS02505 - acyl-CoA dehydrogenase, at 561,906 to 563,693
_RS02505
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
RR42_RS02515: RR42_RS02515 - acetyl-CoA acetyltransferase, at 566,334 to 567,533
_RS02515
Position (kb) 563
564
565
566
567 Strain fitness (log2 ratio) -2
-1
0
1
2 at 563.196 kb on + strand, within RR42_RS02505 at 563.196 kb on + strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.508 kb on + strand, within RR42_RS02505 at 563.528 kb on + strand at 563.568 kb on + strand at 563.569 kb on - strand at 563.569 kb on - strand at 563.598 kb on + strand at 563.598 kb on + strand at 563.712 kb on + strand at 563.724 kb on - strand at 563.740 kb on - strand at 563.740 kb on - strand at 563.772 kb on - strand at 563.803 kb on + strand at 563.804 kb on - strand at 563.878 kb on + strand at 563.878 kb on + strand at 564.117 kb on + strand at 564.202 kb on - strand, within RR42_RS02510 at 564.252 kb on - strand, within RR42_RS02510 at 564.345 kb on + strand, within RR42_RS02510 at 564.346 kb on - strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.813 kb on + strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.966 kb on + strand, within RR42_RS02510 at 565.248 kb on + strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.738 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.359 kb on - strand at 566.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 563,196 + RR42_RS02505 0.72 -1.1 563,196 + RR42_RS02505 0.72 +0.6 563,197 - RR42_RS02505 0.72 -0.7 563,197 - RR42_RS02505 0.72 -0.8 563,226 + RR42_RS02505 0.74 +0.7 563,226 + RR42_RS02505 0.74 -0.3 563,227 - RR42_RS02505 0.74 +1.0 563,227 - RR42_RS02505 0.74 -0.5 563,475 + RR42_RS02505 0.88 +0.4 563,475 + RR42_RS02505 0.88 +0.2 563,476 - RR42_RS02505 0.88 -0.2 563,476 - RR42_RS02505 0.88 -1.7 563,476 - RR42_RS02505 0.88 +0.3 563,508 + RR42_RS02505 0.90 +1.1 563,528 + -0.3 563,568 + +0.4 563,569 - -0.3 563,569 - -0.5 563,598 + +0.7 563,598 + -0.1 563,712 + -0.4 563,724 - +1.3 563,740 - +0.5 563,740 - -1.1 563,772 - +0.2 563,803 + -0.0 563,804 - -0.4 563,878 + +0.5 563,878 + -2.1 564,117 + +1.6 564,202 - RR42_RS02510 0.13 -0.6 564,252 - RR42_RS02510 0.15 -1.5 564,345 + RR42_RS02510 0.19 -1.6 564,346 - RR42_RS02510 0.19 +0.8 564,528 + RR42_RS02510 0.26 +1.2 564,528 + RR42_RS02510 0.26 +0.6 564,690 + RR42_RS02510 0.33 +1.2 564,690 + RR42_RS02510 0.33 -0.3 564,690 + RR42_RS02510 0.33 -0.5 564,762 + RR42_RS02510 0.36 -1.0 564,762 + RR42_RS02510 0.36 -0.1 564,762 + RR42_RS02510 0.36 -1.2 564,813 + RR42_RS02510 0.38 +0.6 564,955 - RR42_RS02510 0.44 -0.3 564,955 - RR42_RS02510 0.44 -1.0 564,966 + RR42_RS02510 0.44 -0.9 565,248 + RR42_RS02510 0.56 -0.2 565,340 - RR42_RS02510 0.60 +1.8 565,340 - RR42_RS02510 0.60 +0.2 565,635 + RR42_RS02510 0.72 +0.1 565,635 + RR42_RS02510 0.72 -1.0 565,737 + RR42_RS02510 0.76 +0.7 565,737 + RR42_RS02510 0.76 -0.3 565,737 + RR42_RS02510 0.76 -0.0 565,737 + RR42_RS02510 0.76 +0.9 565,738 - RR42_RS02510 0.76 +0.4 565,846 - RR42_RS02510 0.81 +0.6 565,846 - RR42_RS02510 0.81 -0.5 566,358 + +0.4 566,358 + +1.6 566,358 + +0.1 566,358 + +0.8 566,358 + -1.6 566,359 - +0.4 566,359 - +0.7
Or see this region's nucleotide sequence