Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02500 and RR42_RS02505 are separated by 73 nucleotides RR42_RS02505 and RR42_RS02510 are separated by 195 nucleotides
RR42_RS02500: RR42_RS02500 - TetR family transcriptional regulator, at 561,242 to 561,832
_RS02500
RR42_RS02505: RR42_RS02505 - acyl-CoA dehydrogenase, at 561,906 to 563,693
_RS02505
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 560.906 kb on + strand at 560.907 kb on - strand at 560.907 kb on - strand at 561.065 kb on - strand at 561.129 kb on + strand at 561.129 kb on + strand at 561.130 kb on - strand at 561.142 kb on + strand at 561.142 kb on + strand at 561.552 kb on - strand, within RR42_RS02500 at 561.552 kb on - strand, within RR42_RS02500 at 561.735 kb on + strand, within RR42_RS02500 at 561.805 kb on + strand at 561.821 kb on + strand at 561.826 kb on + strand at 561.826 kb on + strand at 561.915 kb on + strand at 561.915 kb on + strand at 561.915 kb on + strand at 562.103 kb on + strand, within RR42_RS02505 at 562.108 kb on - strand, within RR42_RS02505 at 562.108 kb on - strand, within RR42_RS02505 at 562.108 kb on - strand, within RR42_RS02505 at 562.155 kb on + strand, within RR42_RS02505 at 562.155 kb on + strand, within RR42_RS02505 at 562.165 kb on - strand, within RR42_RS02505 at 562.165 kb on - strand, within RR42_RS02505 at 562.206 kb on + strand, within RR42_RS02505 at 562.226 kb on + strand, within RR42_RS02505 at 562.248 kb on + strand, within RR42_RS02505 at 562.248 kb on + strand, within RR42_RS02505 at 562.249 kb on - strand, within RR42_RS02505 at 562.249 kb on - strand, within RR42_RS02505 at 562.249 kb on - strand, within RR42_RS02505 at 562.286 kb on - strand, within RR42_RS02505 at 562.288 kb on - strand, within RR42_RS02505 at 562.312 kb on - strand, within RR42_RS02505 at 562.356 kb on + strand, within RR42_RS02505 at 562.356 kb on + strand, within RR42_RS02505 at 562.357 kb on - strand, within RR42_RS02505 at 562.357 kb on - strand, within RR42_RS02505 at 562.357 kb on - strand, within RR42_RS02505 at 562.474 kb on - strand, within RR42_RS02505 at 563.196 kb on + strand, within RR42_RS02505 at 563.196 kb on + strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.508 kb on + strand, within RR42_RS02505 at 563.528 kb on + strand at 563.568 kb on + strand at 563.569 kb on - strand at 563.569 kb on - strand at 563.598 kb on + strand at 563.598 kb on + strand at 563.712 kb on + strand at 563.724 kb on - strand at 563.740 kb on - strand at 563.740 kb on - strand at 563.772 kb on - strand at 563.803 kb on + strand at 563.804 kb on - strand at 563.878 kb on + strand at 563.878 kb on + strand at 564.117 kb on + strand at 564.202 kb on - strand, within RR42_RS02510 at 564.252 kb on - strand, within RR42_RS02510 at 564.345 kb on + strand, within RR42_RS02510 at 564.346 kb on - strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 560,906 + -0.7 560,907 - -1.1 560,907 - +1.2 561,065 - +0.8 561,129 + +0.6 561,129 + +0.5 561,130 - -0.3 561,142 + -0.7 561,142 + -0.0 561,552 - RR42_RS02500 0.52 -1.1 561,552 - RR42_RS02500 0.52 -1.4 561,735 + RR42_RS02500 0.83 -3.2 561,805 + +0.6 561,821 + -0.7 561,826 + -0.1 561,826 + -0.5 561,915 + -0.4 561,915 + +0.2 561,915 + +0.2 562,103 + RR42_RS02505 0.11 -0.3 562,108 - RR42_RS02505 0.11 -1.4 562,108 - RR42_RS02505 0.11 -0.1 562,108 - RR42_RS02505 0.11 -0.1 562,155 + RR42_RS02505 0.14 -2.6 562,155 + RR42_RS02505 0.14 -1.4 562,165 - RR42_RS02505 0.14 +2.7 562,165 - RR42_RS02505 0.14 +0.4 562,206 + RR42_RS02505 0.17 -0.5 562,226 + RR42_RS02505 0.18 +1.6 562,248 + RR42_RS02505 0.19 -0.4 562,248 + RR42_RS02505 0.19 +0.8 562,249 - RR42_RS02505 0.19 -0.0 562,249 - RR42_RS02505 0.19 +0.4 562,249 - RR42_RS02505 0.19 -0.0 562,286 - RR42_RS02505 0.21 +0.2 562,288 - RR42_RS02505 0.21 +1.2 562,312 - RR42_RS02505 0.23 -0.3 562,356 + RR42_RS02505 0.25 -1.2 562,356 + RR42_RS02505 0.25 -2.0 562,357 - RR42_RS02505 0.25 +1.0 562,357 - RR42_RS02505 0.25 -0.5 562,357 - RR42_RS02505 0.25 +0.1 562,474 - RR42_RS02505 0.32 +0.4 563,196 + RR42_RS02505 0.72 -1.1 563,196 + RR42_RS02505 0.72 +0.6 563,197 - RR42_RS02505 0.72 -0.7 563,197 - RR42_RS02505 0.72 -0.8 563,226 + RR42_RS02505 0.74 +0.7 563,226 + RR42_RS02505 0.74 -0.3 563,227 - RR42_RS02505 0.74 +1.0 563,227 - RR42_RS02505 0.74 -0.5 563,475 + RR42_RS02505 0.88 +0.4 563,475 + RR42_RS02505 0.88 +0.2 563,476 - RR42_RS02505 0.88 -0.2 563,476 - RR42_RS02505 0.88 -1.7 563,476 - RR42_RS02505 0.88 +0.3 563,508 + RR42_RS02505 0.90 +1.1 563,528 + -0.3 563,568 + +0.4 563,569 - -0.3 563,569 - -0.5 563,598 + +0.7 563,598 + -0.1 563,712 + -0.4 563,724 - +1.3 563,740 - +0.5 563,740 - -1.1 563,772 - +0.2 563,803 + -0.0 563,804 - -0.4 563,878 + +0.5 563,878 + -2.1 564,117 + +1.6 564,202 - RR42_RS02510 0.13 -0.6 564,252 - RR42_RS02510 0.15 -1.5 564,345 + RR42_RS02510 0.19 -1.6 564,346 - RR42_RS02510 0.19 +0.8 564,528 + RR42_RS02510 0.26 +1.2 564,528 + RR42_RS02510 0.26 +0.6 564,690 + RR42_RS02510 0.33 +1.2 564,690 + RR42_RS02510 0.33 -0.3 564,690 + RR42_RS02510 0.33 -0.5
Or see this region's nucleotide sequence