Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02485 and RR42_RS02490 overlap by 16 nucleotides RR42_RS02490 and RR42_RS02495 are separated by 141 nucleotides RR42_RS02495 and RR42_RS02500 are separated by 369 nucleotides
RR42_RS02485: RR42_RS02485 - short-chain dehydrogenase, at 558,270 to 558,869
_RS02485
RR42_RS02490: RR42_RS02490 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 558,854 to 559,411
_RS02490
RR42_RS02495: RR42_RS02495 - cardiolipin synthase; exported protein, at 559,553 to 560,872
_RS02495
RR42_RS02500: RR42_RS02500 - TetR family transcriptional regulator, at 561,242 to 561,832
_RS02500
Position (kb) 559
560
561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.737 kb on - strand, within RR42_RS02485 at 558.760 kb on - strand, within RR42_RS02485 at 558.855 kb on + strand at 558.976 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.102 kb on - strand, within RR42_RS02490 at 559.449 kb on - strand at 559.449 kb on - strand at 559.790 kb on + strand, within RR42_RS02495 at 559.790 kb on + strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.797 kb on - strand, within RR42_RS02495 at 559.825 kb on - strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 560.190 kb on + strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.532 kb on - strand, within RR42_RS02495 at 560.690 kb on + strand, within RR42_RS02495 at 560.819 kb on + strand at 560.819 kb on + strand at 560.819 kb on + strand at 560.820 kb on - strand at 560.820 kb on - strand at 560.820 kb on - strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.822 kb on - strand at 560.822 kb on - strand at 560.906 kb on + strand at 560.907 kb on - strand at 560.907 kb on - strand at 561.065 kb on - strand at 561.129 kb on + strand at 561.129 kb on + strand at 561.130 kb on - strand at 561.142 kb on + strand at 561.142 kb on + strand at 561.552 kb on - strand, within RR42_RS02500 at 561.552 kb on - strand, within RR42_RS02500 at 561.735 kb on + strand, within RR42_RS02500 at 561.805 kb on + strand at 561.821 kb on + strand at 561.826 kb on + strand at 561.826 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 558,736 + RR42_RS02485 0.78 +1.1 558,736 + RR42_RS02485 0.78 -0.1 558,736 + RR42_RS02485 0.78 -1.7 558,737 - RR42_RS02485 0.78 -1.1 558,760 - RR42_RS02485 0.82 -0.1 558,855 + +0.4 558,976 + RR42_RS02490 0.22 -0.0 559,101 + RR42_RS02490 0.44 -0.9 559,101 + RR42_RS02490 0.44 -1.6 559,102 - RR42_RS02490 0.44 -3.2 559,449 - +0.3 559,449 - -0.1 559,790 + RR42_RS02495 0.18 +0.2 559,790 + RR42_RS02495 0.18 +0.1 559,791 - RR42_RS02495 0.18 +0.1 559,791 - RR42_RS02495 0.18 -2.4 559,791 - RR42_RS02495 0.18 +0.1 559,797 - RR42_RS02495 0.18 +3.5 559,825 - RR42_RS02495 0.21 +0.8 559,953 + RR42_RS02495 0.30 +0.2 559,953 + RR42_RS02495 0.30 +1.2 560,190 + RR42_RS02495 0.48 -0.6 560,406 - RR42_RS02495 0.65 -0.0 560,406 - RR42_RS02495 0.65 +1.2 560,406 - RR42_RS02495 0.65 +0.3 560,513 + RR42_RS02495 0.73 -1.4 560,513 + RR42_RS02495 0.73 -0.4 560,513 + RR42_RS02495 0.73 -0.0 560,532 - RR42_RS02495 0.74 -0.6 560,690 + RR42_RS02495 0.86 -2.6 560,819 + -0.2 560,819 + +0.4 560,819 + +1.1 560,820 - +0.9 560,820 - +0.5 560,820 - +2.1 560,821 + -0.6 560,821 + +1.2 560,821 + -1.1 560,821 + +0.9 560,821 + -0.2 560,821 + -0.1 560,822 - -0.5 560,822 - +1.2 560,906 + -0.7 560,907 - -1.1 560,907 - +1.2 561,065 - +0.8 561,129 + +0.6 561,129 + +0.5 561,130 - -0.3 561,142 + -0.7 561,142 + -0.0 561,552 - RR42_RS02500 0.52 -1.1 561,552 - RR42_RS02500 0.52 -1.4 561,735 + RR42_RS02500 0.83 -3.2 561,805 + +0.6 561,821 + -0.7 561,826 + -0.1 561,826 + -0.5
Or see this region's nucleotide sequence