Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02425 and RR42_RS02430 are separated by 448 nucleotides RR42_RS02430 and RR42_RS02435 are separated by 199 nucleotides RR42_RS02435 and RR42_RS02440 are separated by 14 nucleotides
RR42_RS02425: RR42_RS02425 - hypothetical protein, at 545,742 to 546,350
_RS02425
RR42_RS02430: RR42_RS02430 - sodium:solute symporter, at 546,799 to 548,235
_RS02430
RR42_RS02435: RR42_RS02435 - FmdB family transcriptional regulator, at 548,435 to 548,794
_RS02435
RR42_RS02440: RR42_RS02440 - membrane protein, at 548,809 to 549,507
_RS02440
Position (kb) 546
547
548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 545.967 kb on - strand, within RR42_RS02425 at 546.038 kb on + strand, within RR42_RS02425 at 546.038 kb on + strand, within RR42_RS02425 at 546.039 kb on - strand, within RR42_RS02425 at 546.433 kb on + strand at 546.434 kb on - strand at 546.471 kb on + strand at 546.471 kb on + strand at 546.471 kb on + strand at 546.472 kb on - strand at 546.472 kb on - strand at 546.481 kb on - strand at 546.643 kb on - strand at 546.645 kb on - strand at 546.678 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.918 kb on + strand at 546.918 kb on + strand at 547.079 kb on - strand, within RR42_RS02430 at 547.108 kb on + strand, within RR42_RS02430 at 547.111 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.606 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.837 kb on - strand, within RR42_RS02430 at 547.849 kb on + strand, within RR42_RS02430 at 547.978 kb on + strand, within RR42_RS02430 at 547.979 kb on - strand, within RR42_RS02430 at 547.980 kb on + strand, within RR42_RS02430 at 547.981 kb on + strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 548.434 kb on + strand at 548.444 kb on + strand at 549.093 kb on + strand, within RR42_RS02440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 545,967 - RR42_RS02425 0.37 +0.4 546,038 + RR42_RS02425 0.49 +0.9 546,038 + RR42_RS02425 0.49 +0.1 546,039 - RR42_RS02425 0.49 -0.4 546,433 + +0.5 546,434 - +0.2 546,471 + -0.0 546,471 + -1.0 546,471 + -0.0 546,472 - +1.0 546,472 - -0.7 546,481 - -0.4 546,643 - +1.2 546,645 - +0.2 546,678 + +2.8 546,916 + -0.8 546,916 + -2.2 546,916 + -2.8 546,916 + -0.8 546,916 + -2.6 546,916 + -0.9 546,917 - -0.6 546,917 - -0.5 546,917 - -2.2 546,917 - -1.3 546,917 - -2.2 546,918 + +0.6 546,918 + -1.5 547,079 - RR42_RS02430 0.19 -0.1 547,108 + RR42_RS02430 0.22 -1.3 547,111 + RR42_RS02430 0.22 -0.9 547,366 + RR42_RS02430 0.39 -0.8 547,366 + RR42_RS02430 0.39 -0.2 547,366 + RR42_RS02430 0.39 -1.4 547,366 + RR42_RS02430 0.39 +0.2 547,367 - RR42_RS02430 0.40 -3.0 547,367 - RR42_RS02430 0.40 -0.6 547,367 - RR42_RS02430 0.40 -1.0 547,367 - RR42_RS02430 0.40 -1.1 547,606 + RR42_RS02430 0.56 -0.8 547,681 + RR42_RS02430 0.61 -1.1 547,681 + RR42_RS02430 0.61 -2.3 547,681 + RR42_RS02430 0.61 -1.5 547,681 + RR42_RS02430 0.61 -1.9 547,682 - RR42_RS02430 0.61 -1.1 547,682 - RR42_RS02430 0.61 -0.7 547,682 - RR42_RS02430 0.61 -0.0 547,837 - RR42_RS02430 0.72 -0.9 547,849 + RR42_RS02430 0.73 +0.4 547,978 + RR42_RS02430 0.82 -1.7 547,979 - RR42_RS02430 0.82 -3.0 547,980 + RR42_RS02430 0.82 -1.6 547,981 + RR42_RS02430 0.82 -2.3 547,981 - RR42_RS02430 0.82 +0.3 547,981 - RR42_RS02430 0.82 -2.9 547,981 - RR42_RS02430 0.82 -1.5 548,434 + -0.5 548,444 + +1.9 549,093 + RR42_RS02440 0.41 +0.1
Or see this region's nucleotide sequence