Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02295 and RR42_RS02300 are separated by 123 nucleotides RR42_RS02300 and RR42_RS02305 are separated by 284 nucleotides
RR42_RS02295: RR42_RS02295 - secretion protein HlyD, at 514,054 to 515,007
_RS02295
RR42_RS02300: RR42_RS02300 - hypothetical protein, at 515,131 to 515,802
_RS02300
RR42_RS02305: RR42_RS02305 - porin, at 516,087 to 517,421
_RS02305
Position (kb)
515
516 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 514.163 kb on + strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.388 kb on + strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.429 kb on + strand, within RR42_RS02295 at 514.622 kb on + strand, within RR42_RS02295 at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.920 kb on - strand at 514.960 kb on + strand at 514.960 kb on + strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.241 kb on + strand, within RR42_RS02300 at 515.606 kb on + strand, within RR42_RS02300 at 515.607 kb on - strand, within RR42_RS02300 at 515.607 kb on - strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.610 kb on - strand, within RR42_RS02300 at 515.610 kb on - strand, within RR42_RS02300 at 515.824 kb on + strand at 515.843 kb on - strand at 515.897 kb on - strand at 515.922 kb on + strand at 515.923 kb on - strand at 515.923 kb on - strand at 515.933 kb on + strand at 515.933 kb on + strand at 515.933 kb on + strand at 515.934 kb on - strand at 515.934 kb on - strand at 515.945 kb on + strand at 515.946 kb on - strand at 515.946 kb on - strand at 516.216 kb on + strand at 516.216 kb on + strand at 516.216 kb on + strand at 516.216 kb on + strand at 516.216 kb on + strand at 516.217 kb on - strand at 516.217 kb on - strand at 516.217 kb on - strand at 516.217 kb on - strand at 516.218 kb on + strand at 516.218 kb on + strand at 516.218 kb on + strand at 516.219 kb on - strand at 516.219 kb on - strand at 516.219 kb on - strand at 516.294 kb on + strand, within RR42_RS02305 at 516.295 kb on - strand, within RR42_RS02305 at 516.295 kb on - strand, within RR42_RS02305 at 516.295 kb on - strand, within RR42_RS02305 at 516.436 kb on + strand, within RR42_RS02305 at 516.618 kb on + strand, within RR42_RS02305 at 516.618 kb on + strand, within RR42_RS02305 at 516.702 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 514,163 + RR42_RS02295 0.11 +1.4 514,164 - RR42_RS02295 0.12 +0.5 514,164 - RR42_RS02295 0.12 -1.6 514,202 + RR42_RS02295 0.16 -0.2 514,202 + RR42_RS02295 0.16 -0.1 514,202 + RR42_RS02295 0.16 +0.4 514,202 + RR42_RS02295 0.16 +0.6 514,202 + RR42_RS02295 0.16 +0.0 514,203 - RR42_RS02295 0.16 +1.0 514,203 - RR42_RS02295 0.16 -0.3 514,203 - RR42_RS02295 0.16 +2.1 514,203 - RR42_RS02295 0.16 -0.8 514,203 - RR42_RS02295 0.16 +0.2 514,298 + RR42_RS02295 0.26 -0.7 514,298 + RR42_RS02295 0.26 -0.8 514,298 + RR42_RS02295 0.26 -0.2 514,299 - RR42_RS02295 0.26 -2.0 514,299 - RR42_RS02295 0.26 -1.0 514,299 - RR42_RS02295 0.26 +0.8 514,388 + RR42_RS02295 0.35 -2.4 514,389 - RR42_RS02295 0.35 -2.2 514,389 - RR42_RS02295 0.35 +0.2 514,429 + RR42_RS02295 0.39 -1.6 514,622 + RR42_RS02295 0.60 +0.3 514,919 + -0.8 514,919 + -0.4 514,919 + +0.2 514,919 + +1.7 514,920 - +1.0 514,960 + -0.3 514,960 + +0.7 514,961 - +1.2 514,961 - +1.8 514,961 - -0.3 514,961 - +0.2 515,232 + RR42_RS02300 0.15 -0.7 515,232 + RR42_RS02300 0.15 +1.0 515,232 + RR42_RS02300 0.15 -0.2 515,232 + RR42_RS02300 0.15 +2.6 515,233 - RR42_RS02300 0.15 -0.2 515,233 - RR42_RS02300 0.15 +0.3 515,233 - RR42_RS02300 0.15 -0.1 515,233 - RR42_RS02300 0.15 -1.6 515,241 + RR42_RS02300 0.16 -0.6 515,606 + RR42_RS02300 0.71 +1.0 515,607 - RR42_RS02300 0.71 +0.4 515,607 - RR42_RS02300 0.71 +0.5 515,609 + RR42_RS02300 0.71 +1.0 515,609 + RR42_RS02300 0.71 +1.2 515,609 + RR42_RS02300 0.71 +0.3 515,610 - RR42_RS02300 0.71 +0.7 515,610 - RR42_RS02300 0.71 -1.1 515,824 + +0.9 515,843 - +0.0 515,897 - +0.6 515,922 + -0.4 515,923 - -0.0 515,923 - +0.7 515,933 + -1.4 515,933 + +2.8 515,933 + +1.3 515,934 - +0.9 515,934 - +0.6 515,945 + -0.6 515,946 - -0.0 515,946 - -0.0 516,216 + -1.4 516,216 + -0.4 516,216 + +0.3 516,216 + -1.0 516,216 + -0.3 516,217 - +0.1 516,217 - +0.2 516,217 - -0.2 516,217 - -0.6 516,218 + +0.2 516,218 + +0.4 516,218 + +0.2 516,219 - +0.4 516,219 - +1.2 516,219 - +1.8 516,294 + RR42_RS02305 0.16 -2.2 516,295 - RR42_RS02305 0.16 -1.4 516,295 - RR42_RS02305 0.16 -0.7 516,295 - RR42_RS02305 0.16 -1.2 516,436 + RR42_RS02305 0.26 +1.0 516,618 + RR42_RS02305 0.40 +0.5 516,618 + RR42_RS02305 0.40 +0.6 516,702 + RR42_RS02305 0.46 -0.2 516,726 + RR42_RS02305 0.48 -0.5 516,726 + RR42_RS02305 0.48 +0.7 516,726 + RR42_RS02305 0.48 -0.5
Or see this region's nucleotide sequence