Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02190 and RR42_RS02195 are separated by 2 nucleotides RR42_RS02195 and RR42_RS02200 are separated by 41 nucleotides RR42_RS02200 and RR42_RS02205 are separated by 65 nucleotides RR42_RS02205 and RR42_RS02210 overlap by 4 nucleotides RR42_RS02210 and RR42_RS02215 overlap by 4 nucleotides
RR42_RS02190: RR42_RS02190 - fumarate hydrolyase, at 487,601 to 488,215
_RS02190
RR42_RS02195: RR42_RS02195 - fumarate hydratase, at 488,218 to 489,093
_RS02195
RR42_RS02200: RR42_RS02200 - LacI family transcriptional regulator, at 489,135 to 490,130
_RS02200
RR42_RS02205: RR42_RS02205 - succinate dehydrogenase, at 490,196 to 490,549
_RS02205
RR42_RS02210: RR42_RS02210 - succinate dehydrogenase, at 490,546 to 490,899
_RS02210
RR42_RS02215: RR42_RS02215 - fumarate reductase, at 490,896 to 491,609
_RS02215
Position (kb) 489
490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 488.206 kb on - strand at 488.206 kb on - strand at 488.206 kb on - strand at 488.314 kb on - strand, within RR42_RS02195 at 488.332 kb on + strand, within RR42_RS02195 at 488.335 kb on - strand, within RR42_RS02195 at 488.818 kb on + strand, within RR42_RS02195 at 488.819 kb on - strand, within RR42_RS02195 at 488.876 kb on + strand, within RR42_RS02195 at 488.877 kb on - strand, within RR42_RS02195 at 489.047 kb on + strand at 489.048 kb on - strand at 489.207 kb on + strand at 489.208 kb on - strand at 489.560 kb on - strand, within RR42_RS02200 at 489.560 kb on - strand, within RR42_RS02200 at 489.759 kb on - strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 490.197 kb on + strand at 490.197 kb on + strand at 490.510 kb on - strand, within RR42_RS02205 at 490.566 kb on - strand at 490.856 kb on + strand, within RR42_RS02210 at 490.857 kb on - strand, within RR42_RS02210 at 490.900 kb on - strand at 490.900 kb on - strand at 490.900 kb on - strand at 491.003 kb on + strand, within RR42_RS02215 at 491.004 kb on - strand, within RR42_RS02215
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 488,206 - +0.7 488,206 - +0.9 488,206 - +0.2 488,314 - RR42_RS02195 0.11 +0.1 488,332 + RR42_RS02195 0.13 -0.7 488,335 - RR42_RS02195 0.13 +0.7 488,818 + RR42_RS02195 0.68 +0.1 488,819 - RR42_RS02195 0.69 +0.2 488,876 + RR42_RS02195 0.75 -0.7 488,877 - RR42_RS02195 0.75 +0.1 489,047 + -0.4 489,048 - +2.9 489,207 + +1.4 489,208 - +0.9 489,560 - RR42_RS02200 0.43 -0.9 489,560 - RR42_RS02200 0.43 -0.3 489,759 - RR42_RS02200 0.63 +1.8 489,845 + RR42_RS02200 0.71 +0.8 489,845 + RR42_RS02200 0.71 -1.8 489,845 + RR42_RS02200 0.71 +1.2 489,846 - RR42_RS02200 0.71 -3.2 489,846 - RR42_RS02200 0.71 -0.4 489,848 - RR42_RS02200 0.72 -1.1 489,848 - RR42_RS02200 0.72 +2.2 490,197 + +0.5 490,197 + -0.1 490,510 - RR42_RS02205 0.89 +0.0 490,566 - -0.5 490,856 + RR42_RS02210 0.88 +2.4 490,857 - RR42_RS02210 0.88 +1.2 490,900 - +0.2 490,900 - +0.3 490,900 - -0.1 491,003 + RR42_RS02215 0.15 -0.6 491,004 - RR42_RS02215 0.15 +0.8
Or see this region's nucleotide sequence