Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02040 and RR42_RS02045 are separated by 283 nucleotides RR42_RS02045 and RR42_RS02050 are separated by 99 nucleotides
RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171
_RS02040
RR42_RS02045: RR42_RS02045 - GTPase, at 455,455 to 457,398
_RS02045
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
Position (kb)
455
456
457
458 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 454.464 kb on + strand, within RR42_RS02040 at 454.464 kb on + strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 455.071 kb on - strand, within RR42_RS02040 at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.214 kb on - strand at 455.232 kb on + strand at 455.232 kb on + strand at 455.233 kb on - strand at 455.327 kb on - strand at 455.327 kb on - strand at 455.351 kb on + strand at 455.352 kb on - strand at 455.485 kb on + strand at 455.485 kb on + strand at 455.486 kb on - strand at 455.508 kb on - strand at 455.508 kb on - strand at 455.557 kb on + strand at 455.590 kb on - strand at 455.727 kb on + strand at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.753 kb on - strand, within RR42_RS02045 at 455.853 kb on + strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 456.376 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 457.059 kb on + strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.107 kb on + strand, within RR42_RS02045 at 457.295 kb on + strand at 457.295 kb on + strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.298 kb on + strand at 457.298 kb on + strand at 457.299 kb on - strand at 457.492 kb on + strand at 457.642 kb on + strand, within RR42_RS02050 at 457.702 kb on + strand, within RR42_RS02050 at 457.703 kb on - strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.798 kb on + strand, within RR42_RS02050 at 457.799 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 454,464 + RR42_RS02040 0.20 +1.3 454,464 + RR42_RS02040 0.20 -0.8 454,465 - RR42_RS02040 0.20 +0.6 454,465 - RR42_RS02040 0.20 -0.5 454,792 - RR42_RS02040 0.57 +3.2 454,792 - RR42_RS02040 0.57 +0.8 454,868 + RR42_RS02040 0.66 +0.6 454,868 + RR42_RS02040 0.66 -0.9 454,868 + RR42_RS02040 0.66 -1.1 454,868 + RR42_RS02040 0.66 -2.0 454,868 + RR42_RS02040 0.66 +1.1 454,869 - RR42_RS02040 0.66 +0.5 454,869 - RR42_RS02040 0.66 -0.7 454,869 - RR42_RS02040 0.66 +0.3 454,869 - RR42_RS02040 0.66 +3.4 454,869 - RR42_RS02040 0.66 +0.9 454,869 - RR42_RS02040 0.66 +0.5 454,869 - RR42_RS02040 0.66 +1.4 454,869 - RR42_RS02040 0.66 +0.6 454,872 + RR42_RS02040 0.66 -0.6 454,872 + RR42_RS02040 0.66 -1.1 454,872 + RR42_RS02040 0.66 +0.4 454,872 + RR42_RS02040 0.66 -0.1 454,872 + RR42_RS02040 0.66 +0.5 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 -1.9 454,872 + RR42_RS02040 0.66 +1.4 454,872 + RR42_RS02040 0.66 -0.4 454,872 + RR42_RS02040 0.66 +0.6 454,872 + RR42_RS02040 0.66 -1.1 454,872 + RR42_RS02040 0.66 -3.0 454,873 - RR42_RS02040 0.66 -1.9 454,873 - RR42_RS02040 0.66 +1.0 454,873 - RR42_RS02040 0.66 -0.6 454,873 - RR42_RS02040 0.66 -0.8 454,873 - RR42_RS02040 0.66 +0.1 454,873 - RR42_RS02040 0.66 +1.0 454,873 - RR42_RS02040 0.66 -0.6 454,873 - RR42_RS02040 0.66 +1.6 454,873 - RR42_RS02040 0.66 -1.9 455,071 - RR42_RS02040 0.89 +1.0 455,148 + +0.1 455,148 + +0.1 455,148 + +1.1 455,148 + +0.7 455,214 - -1.6 455,232 + +0.4 455,232 + +0.7 455,233 - +0.7 455,327 - -2.9 455,327 - +0.4 455,351 + +0.2 455,352 - -3.4 455,485 + +0.0 455,485 + +1.2 455,486 - -1.2 455,508 - -3.2 455,508 - -1.6 455,557 + +1.2 455,590 - -1.8 455,727 + -0.4 455,727 + RR42_RS02045 0.14 -4.9 455,727 + RR42_RS02045 0.14 -2.2 455,727 + RR42_RS02045 0.14 -2.7 455,727 + RR42_RS02045 0.14 -4.8 455,728 - RR42_RS02045 0.14 -2.1 455,728 - RR42_RS02045 0.14 -1.7 455,728 - RR42_RS02045 0.14 -1.1 455,728 - RR42_RS02045 0.14 -4.6 455,728 - RR42_RS02045 0.14 -3.2 455,753 - RR42_RS02045 0.15 -3.6 455,853 + RR42_RS02045 0.20 -2.2 455,854 - RR42_RS02045 0.21 -3.5 455,854 - RR42_RS02045 0.21 -3.9 455,959 + RR42_RS02045 0.26 -1.0 455,959 + RR42_RS02045 0.26 -1.5 455,960 - RR42_RS02045 0.26 -3.0 455,960 - RR42_RS02045 0.26 -2.0 456,376 + RR42_RS02045 0.47 -2.7 456,928 + RR42_RS02045 0.76 -2.2 456,928 + RR42_RS02045 0.76 -0.3 456,929 - RR42_RS02045 0.76 -0.6 456,929 - RR42_RS02045 0.76 -5.1 456,929 - RR42_RS02045 0.76 -3.2 456,957 + RR42_RS02045 0.77 -3.1 456,957 + RR42_RS02045 0.77 -3.1 456,957 + RR42_RS02045 0.77 -0.8 456,957 + RR42_RS02045 0.77 -2.9 456,958 - RR42_RS02045 0.77 -0.1 456,958 - RR42_RS02045 0.77 -3.1 456,958 - RR42_RS02045 0.77 -2.4 456,958 - RR42_RS02045 0.77 -3.1 456,958 - RR42_RS02045 0.77 -1.4 456,994 + RR42_RS02045 0.79 -0.8 456,994 + RR42_RS02045 0.79 -2.5 456,994 + RR42_RS02045 0.79 -4.4 456,994 + RR42_RS02045 0.79 -1.9 456,995 - RR42_RS02045 0.79 -2.8 456,995 - RR42_RS02045 0.79 -0.8 457,059 + RR42_RS02045 0.83 -3.1 457,060 - RR42_RS02045 0.83 -2.5 457,060 - RR42_RS02045 0.83 +0.2 457,107 + RR42_RS02045 0.85 -1.2 457,295 + -2.6 457,295 + -3.0 457,296 - -3.6 457,296 - -4.0 457,296 - -3.5 457,298 + -3.0 457,298 + -1.9 457,299 - -2.8 457,492 + +0.3 457,642 + RR42_RS02050 0.12 +0.6 457,702 + RR42_RS02050 0.17 +0.0 457,703 - RR42_RS02050 0.18 -1.2 457,795 + RR42_RS02050 0.25 -0.5 457,795 + RR42_RS02050 0.25 -0.5 457,798 + RR42_RS02050 0.26 +0.1 457,799 - RR42_RS02050 0.26 +0.8 458,108 - RR42_RS02050 0.52 -0.3 458,108 - RR42_RS02050 0.52 -0.8 458,108 - RR42_RS02050 0.52 -0.5
Or see this region's nucleotide sequence