Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS02035

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS02025 and RR42_RS02030 overlap by 4 nucleotidesRR42_RS02030 and RR42_RS02035 are separated by 119 nucleotidesRR42_RS02035 and RR42_RS02040 are separated by 140 nucleotides RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529 _RS02025 RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146 _RS02030 RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149 _RS02035 RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171 _RS02040 Position (kb) 452 453 454 455Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 451.820 kb on - strand, within RR42_RS02030at 452.002 kb on - strand, within RR42_RS02030at 452.002 kb on - strand, within RR42_RS02030at 452.267 kb on + strandat 452.267 kb on + strandat 452.296 kb on - strandat 452.296 kb on - strandat 452.298 kb on - strandat 452.339 kb on - strandat 452.339 kb on - strandat 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.685 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.869 kb on - strand, within RR42_RS02035at 453.018 kb on - strand, within RR42_RS02035at 453.051 kb on - strand, within RR42_RS02035at 453.105 kb on - strand, within RR42_RS02035at 453.117 kb on - strand, within RR42_RS02035at 453.135 kb on - strand, within RR42_RS02035at 453.255 kb on - strand, within RR42_RS02035at 453.891 kb on - strand, within RR42_RS02035at 454.190 kb on - strandat 454.190 kb on - strandat 454.200 kb on + strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.211 kb on + strandat 454.211 kb on + strandat 454.212 kb on - strandat 454.212 kb on - strandat 454.464 kb on + strand, within RR42_RS02040at 454.464 kb on + strand, within RR42_RS02040at 454.465 kb on - strand, within RR42_RS02040at 454.465 kb on - strand, within RR42_RS02040at 454.792 kb on - strand, within RR42_RS02040at 454.792 kb on - strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 455.071 kb on - strand, within RR42_RS02040at 455.148 kb on + strandat 455.148 kb on + strandat 455.148 kb on + strandat 455.148 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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451,820 - RR42_RS02030 0.47 +0.4
452,002 - RR42_RS02030 0.77 +0.8
452,002 - RR42_RS02030 0.77 -1.8
452,267 + -2.4
452,267 + +0.5
452,296 - +0.8
452,296 - +0.3
452,298 - -2.3
452,339 - -0.4
452,339 - -1.4
452,523 - RR42_RS02035 0.14 -1.6
452,523 - RR42_RS02035 0.14 -0.4
452,523 - RR42_RS02035 0.14 -2.6
452,523 - RR42_RS02035 0.14 -2.8
452,622 - RR42_RS02035 0.19 -2.9
452,622 - RR42_RS02035 0.19 -0.6
452,622 - RR42_RS02035 0.19 -1.1
452,622 - RR42_RS02035 0.19 -1.4
452,685 - RR42_RS02035 0.22 -1.0
452,867 - RR42_RS02035 0.32 -1.4
452,867 - RR42_RS02035 0.32 -1.2
452,867 - RR42_RS02035 0.32 -2.4
452,869 - RR42_RS02035 0.32 -4.0
453,018 - RR42_RS02035 0.40 -1.0
453,051 - RR42_RS02035 0.42 -0.8
453,105 - RR42_RS02035 0.45 -1.0
453,117 - RR42_RS02035 0.45 -1.5
453,135 - RR42_RS02035 0.46 -1.8
453,255 - RR42_RS02035 0.52 -0.6
453,891 - RR42_RS02035 0.86 -0.8
454,190 - -0.8
454,190 - +0.2
454,200 + +0.1
454,201 - +0.5
454,201 - +0.1
454,201 - +1.7
454,201 - -0.1
454,211 + -0.7
454,211 + -0.6
454,212 - -0.8
454,212 - +1.6
454,464 + RR42_RS02040 0.20 +1.3
454,464 + RR42_RS02040 0.20 -0.8
454,465 - RR42_RS02040 0.20 +0.6
454,465 - RR42_RS02040 0.20 -0.5
454,792 - RR42_RS02040 0.57 +3.2
454,792 - RR42_RS02040 0.57 +0.8
454,868 + RR42_RS02040 0.66 +0.6
454,868 + RR42_RS02040 0.66 -0.9
454,868 + RR42_RS02040 0.66 -1.1
454,868 + RR42_RS02040 0.66 -2.0
454,868 + RR42_RS02040 0.66 +1.1
454,869 - RR42_RS02040 0.66 +0.5
454,869 - RR42_RS02040 0.66 -0.7
454,869 - RR42_RS02040 0.66 +0.3
454,869 - RR42_RS02040 0.66 +3.4
454,869 - RR42_RS02040 0.66 +0.9
454,869 - RR42_RS02040 0.66 +0.5
454,869 - RR42_RS02040 0.66 +1.4
454,869 - RR42_RS02040 0.66 +0.6
454,872 + RR42_RS02040 0.66 -0.6
454,872 + RR42_RS02040 0.66 -1.1
454,872 + RR42_RS02040 0.66 +0.4
454,872 + RR42_RS02040 0.66 -0.1
454,872 + RR42_RS02040 0.66 +0.5
454,872 + RR42_RS02040 0.66 -0.5
454,872 + RR42_RS02040 0.66 -1.9
454,872 + RR42_RS02040 0.66 +1.4
454,872 + RR42_RS02040 0.66 -0.4
454,872 + RR42_RS02040 0.66 +0.6
454,872 + RR42_RS02040 0.66 -1.1
454,872 + RR42_RS02040 0.66 -3.0
454,873 - RR42_RS02040 0.66 -1.9
454,873 - RR42_RS02040 0.66 +1.0
454,873 - RR42_RS02040 0.66 -0.6
454,873 - RR42_RS02040 0.66 -0.8
454,873 - RR42_RS02040 0.66 +0.1
454,873 - RR42_RS02040 0.66 +1.0
454,873 - RR42_RS02040 0.66 -0.6
454,873 - RR42_RS02040 0.66 +1.6
454,873 - RR42_RS02040 0.66 -1.9
455,071 - RR42_RS02040 0.89 +1.0
455,148 + +0.1
455,148 + +0.1
455,148 + +1.1
455,148 + +0.7

Or see this region's nucleotide sequence