Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02020 and RR42_RS02025 are separated by 59 nucleotides RR42_RS02025 and RR42_RS02030 overlap by 4 nucleotides RR42_RS02030 and RR42_RS02035 are separated by 119 nucleotides
RR42_RS02020: RR42_RS02020 - tRNA-Gln, at 450,515 to 450,591
_RS02020
RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529
_RS02025
RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146
_RS02030
RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149
_RS02035
Position (kb) 451
452
453 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 451.820 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.267 kb on + strand at 452.267 kb on + strand at 452.296 kb on - strand at 452.296 kb on - strand at 452.298 kb on - strand at 452.339 kb on - strand at 452.339 kb on - strand at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.685 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.869 kb on - strand, within RR42_RS02035 at 453.018 kb on - strand, within RR42_RS02035 at 453.051 kb on - strand, within RR42_RS02035 at 453.105 kb on - strand, within RR42_RS02035 at 453.117 kb on - strand, within RR42_RS02035 at 453.135 kb on - strand, within RR42_RS02035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 451,820 - RR42_RS02030 0.47 +0.4 452,002 - RR42_RS02030 0.77 +0.8 452,002 - RR42_RS02030 0.77 -1.8 452,267 + -2.4 452,267 + +0.5 452,296 - +0.8 452,296 - +0.3 452,298 - -2.3 452,339 - -0.4 452,339 - -1.4 452,523 - RR42_RS02035 0.14 -1.6 452,523 - RR42_RS02035 0.14 -0.4 452,523 - RR42_RS02035 0.14 -2.6 452,523 - RR42_RS02035 0.14 -2.8 452,622 - RR42_RS02035 0.19 -2.9 452,622 - RR42_RS02035 0.19 -0.6 452,622 - RR42_RS02035 0.19 -1.1 452,622 - RR42_RS02035 0.19 -1.4 452,685 - RR42_RS02035 0.22 -1.0 452,867 - RR42_RS02035 0.32 -1.4 452,867 - RR42_RS02035 0.32 -1.2 452,867 - RR42_RS02035 0.32 -2.4 452,869 - RR42_RS02035 0.32 -4.0 453,018 - RR42_RS02035 0.40 -1.0 453,051 - RR42_RS02035 0.42 -0.8 453,105 - RR42_RS02035 0.45 -1.0 453,117 - RR42_RS02035 0.45 -1.5 453,135 - RR42_RS02035 0.46 -1.8
Or see this region's nucleotide sequence