Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01525 and RR42_RS01530 are separated by 53 nucleotides RR42_RS01530 and RR42_RS01535 are separated by 4 nucleotides RR42_RS01535 and RR42_RS01540 are separated by 13 nucleotides
RR42_RS01525: RR42_RS01525 - LacI family transcriptional regulator, at 343,426 to 344,427
_RS01525
RR42_RS01530: RR42_RS01530 - isopropylmalate isomerase, at 344,481 to 345,899
_RS01530
RR42_RS01535: RR42_RS01535 - 3-isopropylmalate dehydratase, at 345,904 to 346,551
_RS01535
RR42_RS01540: RR42_RS01540 - alpha/beta hydrolase, at 346,565 to 347,455
_RS01540
Position (kb) 344
345
346 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.191 kb on + strand, within RR42_RS01525 at 344.463 kb on + strand at 344.463 kb on + strand at 344.463 kb on + strand at 344.464 kb on - strand at 344.464 kb on - strand at 344.492 kb on + strand at 344.493 kb on - strand at 344.496 kb on + strand at 344.496 kb on + strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.587 kb on - strand at 344.886 kb on + strand, within RR42_RS01530 at 344.964 kb on + strand, within RR42_RS01530 at 345.045 kb on - strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.211 kb on - strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.461 kb on + strand, within RR42_RS01530 at 345.462 kb on - strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.771 kb on - strand at 345.988 kb on - strand, within RR42_RS01535 at 345.988 kb on - strand, within RR42_RS01535 at 346.044 kb on + strand, within RR42_RS01535 at 346.187 kb on - strand, within RR42_RS01535 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.794 kb on - strand, within RR42_RS01540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 344,005 + RR42_RS01525 0.58 +0.1 344,005 + RR42_RS01525 0.58 -1.3 344,005 + RR42_RS01525 0.58 +0.4 344,005 + RR42_RS01525 0.58 -0.7 344,005 + RR42_RS01525 0.58 +0.6 344,005 + RR42_RS01525 0.58 +0.2 344,006 - RR42_RS01525 0.58 -0.6 344,006 - RR42_RS01525 0.58 +2.9 344,006 - RR42_RS01525 0.58 +1.9 344,006 - RR42_RS01525 0.58 -0.4 344,006 - RR42_RS01525 0.58 +0.1 344,006 - RR42_RS01525 0.58 +0.4 344,006 - RR42_RS01525 0.58 -2.9 344,191 + RR42_RS01525 0.76 -2.0 344,463 + +0.8 344,463 + -0.3 344,463 + -0.5 344,464 - +1.7 344,464 - +0.5 344,492 + +0.5 344,493 - -0.5 344,496 + -1.0 344,496 + -0.3 344,497 - +0.0 344,497 - +1.6 344,497 - +0.4 344,586 + +0.1 344,586 + +0.5 344,586 + -2.0 344,587 - +0.2 344,886 + RR42_RS01530 0.29 +0.4 344,964 + RR42_RS01530 0.34 +0.5 345,045 - RR42_RS01530 0.40 +0.3 345,210 + RR42_RS01530 0.51 +0.3 345,210 + RR42_RS01530 0.51 -0.3 345,211 - RR42_RS01530 0.51 +0.2 345,459 + RR42_RS01530 0.69 -0.4 345,459 + RR42_RS01530 0.69 -0.1 345,461 + RR42_RS01530 0.69 -0.0 345,462 - RR42_RS01530 0.69 +0.4 345,736 + RR42_RS01530 0.88 +0.5 345,736 + RR42_RS01530 0.88 +0.4 345,737 - RR42_RS01530 0.89 +0.4 345,737 - RR42_RS01530 0.89 +1.2 345,771 - +0.3 345,988 - RR42_RS01535 0.13 +1.2 345,988 - RR42_RS01535 0.13 +2.3 346,044 + RR42_RS01535 0.22 -0.1 346,187 - RR42_RS01535 0.44 +0.1 346,691 - RR42_RS01540 0.14 -1.0 346,691 - RR42_RS01540 0.14 +0.7 346,691 - RR42_RS01540 0.14 -0.1 346,793 + RR42_RS01540 0.26 +0.3 346,793 + RR42_RS01540 0.26 +0.3 346,793 + RR42_RS01540 0.26 +1.7 346,794 - RR42_RS01540 0.26 +1.2
Or see this region's nucleotide sequence