Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01330 and RR42_RS01335 are separated by 222 nucleotides RR42_RS01335 and RR42_RS01340 are separated by 39 nucleotides
RR42_RS01330: RR42_RS01330 - Fis family transcriptional regulator, at 301,044 to 302,765
_RS01330
RR42_RS01335: RR42_RS01335 - methylmalonyl-CoA carboxyltransferase, at 302,988 to 304,535
_RS01335
RR42_RS01340: RR42_RS01340 - hypothetical protein, at 304,575 to 305,597
_RS01340
Position (kb) 302
303
304
305 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 302.304 kb on + strand, within RR42_RS01330 at 302.740 kb on - strand at 302.740 kb on - strand at 302.767 kb on - strand at 302.873 kb on + strand at 302.873 kb on + strand at 302.874 kb on - strand at 302.938 kb on + strand at 302.939 kb on - strand at 302.951 kb on - strand at 302.963 kb on + strand at 303.172 kb on - strand, within RR42_RS01335 at 303.420 kb on + strand, within RR42_RS01335 at 303.547 kb on - strand, within RR42_RS01335 at 303.619 kb on + strand, within RR42_RS01335 at 303.620 kb on - strand, within RR42_RS01335 at 303.656 kb on + strand, within RR42_RS01335 at 303.733 kb on - strand, within RR42_RS01335 at 303.844 kb on - strand, within RR42_RS01335 at 303.844 kb on - strand, within RR42_RS01335 at 304.161 kb on + strand, within RR42_RS01335 at 304.235 kb on + strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.318 kb on - strand, within RR42_RS01335 at 304.568 kb on + strand at 304.568 kb on + strand at 304.569 kb on - strand at 304.569 kb on - strand at 304.569 kb on - strand at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.121 kb on - strand, within RR42_RS01340 at 305.168 kb on + strand, within RR42_RS01340 at 305.175 kb on + strand, within RR42_RS01340 at 305.175 kb on + strand, within RR42_RS01340 at 305.176 kb on - strand, within RR42_RS01340 at 305.361 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.389 kb on - strand, within RR42_RS01340 at 305.389 kb on - strand, within RR42_RS01340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 302,304 + RR42_RS01330 0.73 +0.3 302,740 - +1.7 302,740 - -0.4 302,767 - -0.9 302,873 + +1.8 302,873 + +1.8 302,874 - +0.4 302,938 + -0.5 302,939 - -0.1 302,951 - +0.4 302,963 + +2.9 303,172 - RR42_RS01335 0.12 +3.0 303,420 + RR42_RS01335 0.28 -0.8 303,547 - RR42_RS01335 0.36 -1.0 303,619 + RR42_RS01335 0.41 -0.4 303,620 - RR42_RS01335 0.41 -1.9 303,656 + RR42_RS01335 0.43 -0.1 303,733 - RR42_RS01335 0.48 +0.8 303,844 - RR42_RS01335 0.55 -0.2 303,844 - RR42_RS01335 0.55 -0.2 304,161 + RR42_RS01335 0.76 +1.3 304,235 + RR42_RS01335 0.81 -0.1 304,236 - RR42_RS01335 0.81 +1.3 304,236 - RR42_RS01335 0.81 -0.3 304,236 - RR42_RS01335 0.81 -0.1 304,317 + RR42_RS01335 0.86 +0.6 304,317 + RR42_RS01335 0.86 +1.1 304,317 + RR42_RS01335 0.86 +0.4 304,318 - RR42_RS01335 0.86 -0.3 304,568 + +0.6 304,568 + -2.5 304,569 - +0.2 304,569 - +0.7 304,569 - +1.3 305,120 + RR42_RS01340 0.53 -1.6 305,120 + RR42_RS01340 0.53 +0.1 305,120 + RR42_RS01340 0.53 +1.6 305,120 + RR42_RS01340 0.53 -0.7 305,120 + RR42_RS01340 0.53 +0.3 305,121 - RR42_RS01340 0.53 +1.1 305,168 + RR42_RS01340 0.58 +1.5 305,175 + RR42_RS01340 0.59 +0.5 305,175 + RR42_RS01340 0.59 -1.1 305,176 - RR42_RS01340 0.59 +0.5 305,361 + RR42_RS01340 0.77 +0.2 305,388 + RR42_RS01340 0.79 -0.2 305,388 + RR42_RS01340 0.79 +0.3 305,388 + RR42_RS01340 0.79 -1.8 305,389 - RR42_RS01340 0.80 +0.3 305,389 - RR42_RS01340 0.80 +2.0
Or see this region's nucleotide sequence