Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01260 and RR42_RS01265 are separated by 385 nucleotides RR42_RS01265 and RR42_RS01270 are separated by 332 nucleotides
RR42_RS01260: RR42_RS01260 - lauroyl acyltransferase, at 284,503 to 285,441
_RS01260
RR42_RS01265: RR42_RS01265 - S-adenosylmethionine synthetase, at 285,827 to 286,990
_RS01265
RR42_RS01270: RR42_RS01270 - 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) hydroxylase, at 287,323 to 288,198
_RS01270
Position (kb) 285
286
287 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 285.057 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.234 kb on - strand, within RR42_RS01260 at 285.481 kb on - strand at 285.481 kb on - strand at 285.481 kb on - strand at 285.537 kb on - strand at 285.537 kb on - strand at 285.551 kb on - strand at 285.689 kb on - strand at 285.760 kb on + strand at 285.766 kb on + strand at 285.787 kb on + strand at 285.787 kb on + strand at 286.988 kb on + strand at 286.988 kb on + strand at 287.132 kb on + strand at 287.341 kb on + strand at 287.341 kb on + strand at 287.343 kb on + strand at 287.343 kb on + strand at 287.344 kb on - strand at 287.344 kb on - strand at 287.572 kb on + strand, within RR42_RS01270 at 287.572 kb on + strand, within RR42_RS01270 at 287.572 kb on + strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.600 kb on - strand, within RR42_RS01270 at 287.600 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.914 kb on - strand, within RR42_RS01270 at 287.914 kb on - strand, within RR42_RS01270 at 287.938 kb on + strand, within RR42_RS01270 at 287.938 kb on + strand, within RR42_RS01270 at 287.939 kb on - strand, within RR42_RS01270 at 287.989 kb on + strand, within RR42_RS01270 at 287.990 kb on - strand, within RR42_RS01270 at 287.990 kb on - strand, within RR42_RS01270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 285,057 - RR42_RS01260 0.59 -2.4 285,198 - RR42_RS01260 0.74 -5.2 285,198 - RR42_RS01260 0.74 -2.7 285,198 - RR42_RS01260 0.74 -3.9 285,234 - RR42_RS01260 0.78 -3.8 285,481 - -0.7 285,481 - +0.2 285,481 - -1.1 285,537 - -0.7 285,537 - -0.7 285,551 - +0.1 285,689 - -1.7 285,760 + -3.1 285,766 + -0.9 285,787 + -2.7 285,787 + -1.6 286,988 + -0.7 286,988 + -0.1 287,132 + +0.2 287,341 + +0.3 287,341 + -2.0 287,343 + -1.0 287,343 + -2.4 287,344 - -4.4 287,344 - -1.6 287,572 + RR42_RS01270 0.28 -2.2 287,572 + RR42_RS01270 0.28 -2.5 287,572 + RR42_RS01270 0.28 -1.0 287,573 - RR42_RS01270 0.29 -0.8 287,573 - RR42_RS01270 0.29 -2.4 287,573 - RR42_RS01270 0.29 -1.1 287,600 - RR42_RS01270 0.32 -3.1 287,600 - RR42_RS01270 0.32 -2.3 287,647 - RR42_RS01270 0.37 -1.9 287,647 - RR42_RS01270 0.37 +0.3 287,647 - RR42_RS01270 0.37 -1.2 287,913 + RR42_RS01270 0.67 +1.3 287,913 + RR42_RS01270 0.67 -1.9 287,913 + RR42_RS01270 0.67 -3.6 287,913 + RR42_RS01270 0.67 +2.3 287,914 - RR42_RS01270 0.67 -2.4 287,914 - RR42_RS01270 0.67 -3.0 287,938 + RR42_RS01270 0.70 -1.9 287,938 + RR42_RS01270 0.70 -2.4 287,939 - RR42_RS01270 0.70 -3.0 287,989 + RR42_RS01270 0.76 -1.8 287,990 - RR42_RS01270 0.76 -1.3 287,990 - RR42_RS01270 0.76 -2.5
Or see this region's nucleotide sequence